|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
|
|
If you have enough headspace in the top of the container, you can colonize a plate just fine. Cultures on agar require very little GE.
I don't use the containers pasty does but I keep all my petris in ziplock bags, 2-3 per bag, they have no issues
|
elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
|
|
Quote:
FrankHorrigan said: If you have enough headspace in the top of the container, you can colonize a plate just fine. Cultures on agar require very little GE.
I don't use the containers pasty does but I keep all my petris in ziplock bags, 2-3 per bag, they have no issues 
I know you can't PC something that's completely sealed without getting the vacuum. I guess what I was trying to ask was 'how much GE is really necessary for a culture?' Surely a quarter inch hole with micropore tape is a little over kill? Not saying GE is bad for cultures. But how much is really necessary when you're just talking about a little bit of myc on a tiny plate?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
|
|
I found that the quarter inch was what worked for me to keep the container from being crushed in. It does not really matter if they get a bit squished anyway, just add a little extra agar to ensure complete coverage and use a smaller hole if you want to. But I would not recommend no hole. No hole is no bueno.
|
elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
|
|
Quote:
Pastywhyte said: No hole is no bueno.
Sorry that sounds so dirty.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
I made some PF cakes in the mini rounds that you do your agar in without a GE hole, what a fucking mess when I opened up the PC
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
|
|
Quote:
bodhisatta said: I made some PF cakes in the mini rounds that you do your agar in without a GE hole, what a fucking mess when I opened up the PC
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 5 hours
|
|
Quote:
FrankHorrigan said: I don't use the containers pasty does but I keep all my petris in ziplock bags, 2-3 per bag, they have no issues 
Ziplock bags are not air tight.
At least they never are for me.
I always squeeze the air out before sealing, but the air always finds its way back in.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
|
|
You must have some crappy bags
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,325
Loc: Dept of know what I'm say...
Last seen: 1 day, 11 hours
|
|
I've stored uninoculated petris in ziplock bags for a couple months with no problems. Buy the ziplock brand. The off brand are shitty
I actually put all my petris in ziplock bags. I don't use parafilm. Never had a problem
|
FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
|
|
Tell ya what pussy, I'll vac-seal a bag around a dish next time I make transfers.
Obviously I won't suck all the air out, but it will definitely be an air-tight seal.
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 5 hours
|
|
I think I'll do the same on my next batch of transfers, just to see ya know.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
|
|
Let's do it.
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,325
Loc: Dept of know what I'm say...
Last seen: 1 day, 11 hours
|
|
Yall are weird
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 5 hours
|
|
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,325
Loc: Dept of know what I'm say...
Last seen: 1 day, 11 hours
|
|
exactly
|
DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
|
|
So I follow doc T's agar tek. Would I benefit adding some honey to it? http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981
I usually do the small batch enough for 15 jars worth. If so how much honey should I add to that mix? I Wana try yours pasty but I don't have potato flakes atm.
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
|
Re: Pasty Agar Tek [Re: DeadPhan]
#19721156 - 03/19/14 09:12 PM (9 years, 10 months ago) |
|
|
If your using mea I wouldn't bother with honey. Really the only reason I used PDA for this tek is that its pretty simple to make and the ingredients are easy to find. I like to rotate my media and use PDA, MEA, cornstarch agar, soak water from wheat rye or oats, and I did dog food once. Next one I wanna try is rice krispies agar
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
pasty how much agar do u use if u are making a sleeve (25) of regular petris? for every 500ml of water i mean.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
|
|
Quote:
blindingleaf said: pasty how much agar do u use if u are making a sleeve (25) of regular petris? for every 500ml of water i mean.
Are you using MEA powder or making the medium from scratch? If MEA powder then 20 grams per 500ml is just fine. Never mixed it all from scratch myself though. I did 8 plates tonight with 7 grams of MEA powder and 180 ML of water and it was just fine. You can fudge it a little.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
|
Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
|
|
I use 10 grams of extra light malt extract and 10 grams telephone brand powdered agar in 500 ml aka a 16.9 oz water bottles worth of water.
|
|