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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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LOL then i fucked up. i used like 6 CC's in 6 rounds.... f'd up.
I guess we'll try it and see what happens..
fyi--- I'm doing flame sterilization in OPEN AIR.... I squirted out the dead stuff and then added the rest into the rounds. I assume the hot needle will kill anyhting that touches it anyways. (why does too much matter??)
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insanemike

Registered: 02/23/14
Posts: 4,272
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Quote:
new2doinstuff said: No. I was told with the screw tops just to leave them loose. The reason you do that with the mini rounds is pressure.
Maybe you're using the word loose loosely but it shouldn't be loose. It just needs to be loosened enough for gas exchange and no more.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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Quote:
insanemike said:
Quote:
new2doinstuff said: No. I was told with the screw tops just to leave them loose. The reason you do that with the mini rounds is pressure.
Maybe you're using the word loose loosely but it shouldn't be loose. It just needs to be loosened enough for gas exchange and no more.
yeah. i could lift it by the lids, just a TINY bit wobbly
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insanemike

Registered: 02/23/14
Posts: 4,272
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No more than a 1/4 turn at the very most.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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yeah it wasnt much more than that, if that
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insanemike

Registered: 02/23/14
Posts: 4,272
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Shouldn't even be that loose. It only needs the GE during PC run. After that it can be tightened right up as there is enough air in the container to support a small colony.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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yeah that's exactly what i did, pasty. i turned them immediately
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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I guess since i used so much, i'll just use a quarter of the agar plate to innoc a jar, maybe a third... if they're gonna be that strong, i guess we'll see how it goes. fuggit
If i need to redo it, no big deal.
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insanemike

Registered: 02/23/14
Posts: 4,272
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You're definitely right about that pasty. I used to not even use a ge hole or filter on the mini rounds and they would colonize just fine.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Remember more genetics is not better. Less is more. With that much I would do at least one transfer to narrow the genetics and also ensure a clean culture. The more solution you use the greater chance of a contam slipping in.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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Hmmmm..... Maybe i;ll do the mini rounds anyways. I used these ones because i bought them on accident, and figured id do it anyways..
I dont know how to transfer.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Look up Munchauzen's videos.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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what do i do with the needle and tip after i use them
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
new2doinstuff said: what do i do with the needle and tip after i use them

Cap and put away..?
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-
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ShroominMe
Stranger
Registered: 05/03/15
Posts: 525
Last seen: 8 years, 3 months
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Quote:
new2doinstuff said: well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-
Might want to get the cats out of your room when you're doing mycology stuffs
Just put in a clean plastic bag and store in your fridge. Cap is nice but not essential.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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Done. Now, i cant find these Munchauzen videos. I'm gonna have to transfer these. each one is a CC or so...
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Just save and PC later when you wanna reuse it.
Quote:
ShroominMe said:
Quote:
new2doinstuff said: well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-
Might want to get the cats out of your room when you're doing mycology stuffs
Just put in a clean plastic bag and store in your fridge. Cap is nice but not essential.
Poke holes in the bag so the cat can breath 
The videos are in your threads list. You've commented in the thread before. http://www.shroomery.org/forums/showflat.php/Number/21032261/fpart/1/vc/1
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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new2doinstuff
Traveller


Registered: 06/15/14
Posts: 264
Last seen: 5 years, 9 months
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ohhh neat! i had no idea haha thanks man.
lol@cats in a bag
i just watched those.
he barely transfers anything... so as a question if i did 1cc per jar, how many transfers do i need PER JAR once is starts to grow? 0.0
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