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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #21794981 - 06/11/15 09:03 PM (8 years, 7 months ago)

LOL then i fucked up. i used like 6 CC's in 6 rounds.... f'd up.


I guess we'll try it and see what happens..

fyi--- I'm doing flame sterilization in OPEN AIR....
I squirted out the dead stuff and then added the rest into the rounds. I assume the hot needle will kill anyhting that touches it anyways.
(why does too much matter??)


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: new2doinstuff]
    #21794983 - 06/11/15 09:03 PM (8 years, 7 months ago)

Quote:

new2doinstuff said:
No. I was told with the screw tops just to leave them loose. The reason you do that with the mini rounds is pressure.




Maybe you're using the word loose loosely but it shouldn't be loose. It just needs to be loosened enough for gas exchange and no more.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: insanemike]
    #21794989 - 06/11/15 09:04 PM (8 years, 7 months ago)

Quote:

insanemike said:
Quote:

new2doinstuff said:
No. I was told with the screw tops just to leave them loose. The reason you do that with the mini rounds is pressure.




Maybe you're using the word loose loosely but it shouldn't be loose. It just needs to be loosened enough for gas exchange and no more.




yeah. i could lift it by the lids, just a TINY bit wobbly


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: new2doinstuff]
    #21794996 - 06/11/15 09:08 PM (8 years, 7 months ago)

No more than a 1/4 turn at the very most.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795001 - 06/11/15 09:09 PM (8 years, 7 months ago)

yeah it wasnt much more than that, if that


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795004 - 06/11/15 09:10 PM (8 years, 7 months ago)

:thumbup:


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: insanemike]
    #21795005 - 06/11/15 09:11 PM (8 years, 7 months ago)

Shouldn't even be that loose. It only needs the GE during PC run. After that it can be tightened right up as there is enough air in the container to support a small colony.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #21795008 - 06/11/15 09:12 PM (8 years, 7 months ago)

yeah that's exactly what i did, pasty. i turned them immediately


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795013 - 06/11/15 09:13 PM (8 years, 7 months ago)

I guess since i used so much, i'll just use a quarter of the agar plate to innoc a jar, maybe a third... if they're gonna be that strong, i guess we'll see how it goes. fuggit

If i need to redo it, no big deal.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: Pastywhyte]
    #21795018 - 06/11/15 09:15 PM (8 years, 7 months ago)

You're definitely right about that pasty. I used to not even use a ge hole or filter on the mini rounds and they would colonize just fine.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795024 - 06/11/15 09:17 PM (8 years, 7 months ago)

Remember more genetics is not better. Less is more. With that much I would do at least one transfer to narrow the genetics and also ensure a clean culture.  The more solution you use the greater chance of a contam slipping in.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #21795037 - 06/11/15 09:20 PM (8 years, 7 months ago)

Hmmmm..... Maybe i;ll do the mini rounds anyways. I used these ones because i bought them on accident, and figured id do it anyways..

I dont know how to transfer.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795044 - 06/11/15 09:21 PM (8 years, 7 months ago)

Look up Munchauzen's videos.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: insanemike]
    #21795070 - 06/11/15 09:26 PM (8 years, 7 months ago)

what do i do with the needle and tip after i use them


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795114 - 06/11/15 09:37 PM (8 years, 7 months ago)

Quote:

new2doinstuff said:
what do i do with the needle and tip after i use them




:confused:

Cap and put away..?


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795139 - 06/11/15 09:45 PM (8 years, 7 months ago)

well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-


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OfflineShroominMe
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795155 - 06/11/15 09:50 PM (8 years, 7 months ago)

Quote:

new2doinstuff said:
well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-



Might want to get the cats out of your room when you're doing mycology stuffs :uhoh:

Just put in a clean plastic bag and store in your fridge.  Cap is nice but not essential.


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795210 - 06/11/15 10:05 PM (8 years, 7 months ago)

Done. Now, i cant find these Munchauzen videos. I'm gonna have to transfer these. each one is a CC or so...


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InvisibleGrey
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Re: Pasty Agar Tek [Re: ShroominMe]
    #21795237 - 06/11/15 10:11 PM (8 years, 7 months ago)

Just save and PC later when you wanna reuse it.

Quote:

ShroominMe said:
Quote:

new2doinstuff said:
well after looking for the cap, im pretty sure my cats contammed the fuck out of it... they knocked it off the table when i walked away and it went under the stove -_-



Might want to get the cats out of your room when you're doing mycology stuffs :uhoh:

Just put in a clean plastic bag and store in your fridge.  Cap is nice but not essential.




Poke holes in the bag so the cat can breath :thumbup:

The videos are in your threads list. You've commented in the thread before. http://www.shroomery.org/forums/showflat.php/Number/21032261/fpart/1/vc/1


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Offlinenew2doinstuff
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Re: Pasty Agar Tek [Re: new2doinstuff]
    #21795269 - 06/11/15 10:19 PM (8 years, 7 months ago)

ohhh neat! i had no idea haha thanks man.


lol@cats in a bag


i just watched those.

he barely transfers anything... so as a question if i did 1cc per jar, how many transfers do i need PER JAR once is starts to grow? 0.0


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