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Invisibleabductee
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Re: Pasty Agar Tek [Re: elasticaltiger]
    #21750122 - 06/01/15 07:48 PM (8 years, 7 months ago)

here's a syringe shot

top of the sheet that came with it.Now I did not fallow any of these instructions.. I read it but fallowed instructions from youtube and here.



so this looks like bacteria? it seems to come from a point and spread out, its nice and white, but doesnt have any of those branches going out.




Edited by abductee (06/01/15 08:20 PM)


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Invisibleabductee
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Re: Pasty Agar Tek [Re: abductee]
    #21750145 - 06/01/15 07:53 PM (8 years, 7 months ago)

I'm very intrigued about the science behind it, and the practices and procedures. I might be trying to go to fast to see some results, and I kinda knew in the back of my head this wasnt stored properly. I tried another method to obtain spores. I should jump on that tonight while i'm doing some shopping on ebay. lol however, I wont be looking for spores on ebay.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: abductee]
    #21750204 - 06/01/15 08:07 PM (8 years, 7 months ago)

Your first plate from spores may not show any sectoring at all. Just take from the outer edges and transfer to another plate and take samples of the most agressive sectors from that plate and transfer them. From there you could inoculate grain or try to further isolate.


Edited by insanemike (06/01/15 08:16 PM)


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Invisibleabductee
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Re: Pasty Agar Tek [Re: insanemike]
    #21750250 - 06/01/15 08:18 PM (8 years, 7 months ago)

sounds good insanemike. tomorrow I'll be working with it and i'll get a better look when i open them up. to transfer and inoculate.. it wont hurt to try and see what happens. :smile:


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Invisibleelasticaltiger
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Re: Pasty Agar Tek [Re: abductee]
    #21750532 - 06/01/15 09:24 PM (8 years, 7 months ago)

How old is that plate abductee?  The age can give you a good idea if it's mycelium or not.  A fully colonized plate like that should take at least 8 - 10 days from spores. If it's faster than that it would definitely be suspect.


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Invisibleabductee
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Re: Pasty Agar Tek [Re: elasticaltiger]
    #21750563 - 06/01/15 09:31 PM (8 years, 7 months ago)

7 days.., I think i'll get a better look tomorrow in the sab at the other plates, its possible there could be some mycelium growing, i'll go over some more pics of mycelium on agar and get a better idea of what im looking for.. Sometimes I'll see a pic and it looks good and then I read a comment saying its mold or web.. I'll just have to learn the points to properly identify the right growth during first plates from ms.


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InvisibleGrey
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Re: Pasty Agar Tek [Re: abductee]
    #21750585 - 06/01/15 09:38 PM (8 years, 7 months ago)

Here's some reference photos to gander at.




This one had a nasty little contam at 1 o' clock. (Galindoi mycelium)



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Offlinespacemanspiff
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Re: Pastywhyte's Easy Agar Tek [Re: pablokabute]
    #21750876 - 06/01/15 10:47 PM (8 years, 7 months ago)

thanks pasty!


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Invisibleabductee
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Re: Pastywhyte's Easy Agar Tek [Re: spacemanspiff]
    #21751598 - 06/02/15 06:56 AM (8 years, 7 months ago)

Awesome pics grey!


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OfflineTuff_Guy2
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Re: Pastywhyte's Easy Agar Tek [Re: abductee]
    #21757091 - 06/03/15 11:32 AM (8 years, 7 months ago)

Serious question guys...

I forgot to add honey to the mix and already filled up my 8 mini tupperwares. Should I re-do everything and add honey this time or will I be fine?


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Tuff_Guy2]
    #21757259 - 06/03/15 12:32 PM (8 years, 7 months ago)

It will be fine.


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OfflineTuff_Guy2
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #21757319 - 06/03/15 12:56 PM (8 years, 7 months ago)

Quote:

Pastywhyte said:
It will be fine.




Ok cool, cuz PC about to be done in 3mins haha. Nice tek though man! Hope this works out for me, I've followed it almost to a T (the honey part :frown:)


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Invisibleabductee
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Re: Pastywhyte's Easy Agar Tek [Re: Tuff_Guy2]
    #21757721 - 06/03/15 03:04 PM (8 years, 7 months ago)

I mentioned I was going to post some better pics of my ms on agar..
"Viewer Discretion is advised"
  Its not pretty.lol







It looks disgusting, needless to say I discarded them.


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Invisiblemush madness
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Re: Pastywhyte's Easy Agar Tek [Re: abductee]
    #21757734 - 06/03/15 03:06 PM (8 years, 7 months ago)

Quote:

abductee said:
I mentioned I was going to post some better pics of my ms on agar..
"Viewer Discretion is advised"
  Its not pretty.lol







It looks disgusting, needless to say I discarded them.





Those look like they come from some guy named Ralph


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Invisibleabductee
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Re: Pastywhyte's Easy Agar Tek [Re: mush madness]
    #21757740 - 06/03/15 03:08 PM (8 years, 7 months ago)

Lol Possibly, a Canadian head shop vendor..
waiting on stuff from Ralph or some word back via email.. i've basically counted it as a loss.. Going to try sporeworks.


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Invisiblemush madness
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Re: Pastywhyte's Easy Agar Tek [Re: abductee]
    #21757757 - 06/03/15 03:13 PM (8 years, 7 months ago)

Quote:

abductee said:
Lol Possibly, a Canadian head shop vendor..
waiting on stuff from Ralph or some word back via email.. i've basically counted it as a loss.. Going to try sporeworks.




I would, only place of the 3 i ordered from that doesn't need lots of agar work. Actually out of the 6 that I got from works none showed bacteria


Edited by mush madness (06/03/15 03:15 PM)


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Invisibleabductee
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Re: Pastywhyte's Easy Agar Tek [Re: mush madness]
    #21757788 - 06/03/15 03:22 PM (8 years, 7 months ago)

They were the first place I tried placing an order with but when I went to check out the price was in U.s dollars instead of Canadian as was advertised..... I'll try again this evening. I found some glass petri dishes on ebay 21 bucks canadian for five, free shipping. :laugh:


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Invisiblemush madness
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Re: Pastywhyte's Easy Agar Tek [Re: abductee]
    #21757851 - 06/03/15 03:37 PM (8 years, 7 months ago)

Quote:

abductee said:
They were the first place I tried placing an order with but when I went to check out the price was in U.s dollars instead of Canadian as was advertised..... I'll try again this evening. I found some glass petri dishes on ebay 21 bucks canadian for five, free shipping. :laugh:




I ordered them too, I still use the plastic ones over them. The lids dont fit well, great for prints but I like a tight fitting lid and they had about 10 to 12 mm play


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OfflineCenterfinger
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Re: Pastywhyte's Easy Agar Tek [Re: mush madness]
    #21757900 - 06/03/15 03:54 PM (8 years, 7 months ago)

I have been reading bits and pieces of this thread all day and I'm going to rock some agar tonight.  I've got a few prints laying around as well as some dried mic from a completely sealed agar dish from two years ago that I want to resurrect.

I probably should have just went with the glad mini containers in the OP, but I saw that Ball has 4oz spice shaker jars available to opted for those.  Sucks that I had to buy more lids though because I don't think the shaker lids would work well with my intended purpose.

Thanks for the tek as I haven't done any agar in over 2 years and this seems easy enough.


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Invisibleabductee
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Re: Pastywhyte's Easy Agar Tek [Re: Centerfinger]
    #21758024 - 06/03/15 04:25 PM (8 years, 7 months ago)

"
Quote:

abductee said:
They were the first place I tried placing an order with but when I went to check out the price was in U.s dollars instead of Canadian as was advertised..... I'll try again this evening. I found some glass petri dishes on ebay 21 bucks canadian for five, free shipping. :laugh:



I ordered them too, I still use the plastic ones over them. The lids dont fit well, great for prints but I like a tight fitting lid and they had about 10 to 12 mm play"

Yeah, I noticed in the picture there was a decent size gap between the halves when the lid is on.


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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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