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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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wiping the pasty plate with the paper towel included in the tek worked for me i can see if i still have the plate, it never contaminated. the paper towel gets sterilized too I'm assuming, and since it is covered in foil, it remains contam free. i don't take the foil off until I'm in the SAB.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Personally I have never really had issues with condesation with these. Maybe cause it is so goddam dry here. But the paper towel should be sterilized, and I did get condensation with pretty much every other no pour container that I tried. A stiffer agar also helps as the moisture on top will usually get reabsorbed back in.
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DrZDA
The ONE

Registered: 02/06/14
Posts: 21
Last seen: 7 years, 3 days
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Re: Pastywhyte's "Easy Agar" Tek [Re: Pastywhyte]
#19562787 - 02/13/14 06:31 PM (9 years, 11 months ago) |
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So legit mang, i was fosho not even going to mess with this side of it until meow man!
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pastywhyte's "Easy Agar" Tek [Re: DrZDA]
#19562846 - 02/13/14 06:49 PM (9 years, 11 months ago) |
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Quote:
A stiffer agar also helps as the moisture on top will usually get reabsorbed back in.
interesting. ill try that.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 4 months
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Quote:
blindingleaf said: wiping the pasty plate with the paper towel included in the tek worked for me i can see if i still have the plate, it never contaminated. the paper towel gets sterilized too I'm assuming, and since it is covered in foil, it remains contam free. i don't take the foil off until I'm in the SAB.
Yeah, I only did the gauze thing because I had no paper towel…I had tried it Violet's way with the screw tops and it didn't work out for me. This time I did the full Pasty plate with the hole and the micropore tape. I should've let the agar sit out to cool with the lid off until the steam was fully evaporated before PCing it, because now I've got condensation again.
It's not nearly as bad though, and it can be remedied…with the paper towel.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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synista



Registered: 08/21/11
Posts: 223
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Quote:
Delection said: You might want to check these out Pasty.
I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene). This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.
I have run a test batch and they did hold up at 15psi for 15 min. I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!).
Here are some pics below.
Packaging

I just got these today, will test them out as well. I was thinking of cutting a groove or two on the edges of the little cups, so when the lid is tight, there is a bit of room for GE, but since the lid will cover the edge, it'd be hard for contams to penetrate. Kind of like screw-ons let air escape.
What do you think about that? I'm not sure if it's clear... the edge would kind of look like a rook. My only issue with these is that there's the plastic "nipple" at the bottom so they wobble when on a surface, but that can just be sanded.
They also have a tad bit bigger ones at my dollar store as well.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: synista]
#19596379 - 02/20/14 07:04 PM (9 years, 11 months ago) |
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Only one way to know, prep a couple then leave em out for a few days. If they contam then no bueno. If not then start inoculating.
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synista



Registered: 08/21/11
Posts: 223
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Well you see, I've never done any agar, so I'm afraid that I might introduce more variables that could make things go wrong (therefore passively suggesting that Delection does the experimenting :P). From my understanding of how contams behave, it *should* work, but you're right, only one way to know.
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Valyr
Con nulla non si fa nulla.


Registered: 05/08/13
Posts: 381
Last seen: 6 years, 9 months
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Re: Pasty Agar Tek [Re: synista]
#19597041 - 02/20/14 09:52 PM (9 years, 11 months ago) |
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Do it the proven way first till you get a hand for it. Then experiment. Probably pretty sound advice for anything to do with this hobby lol.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: Valyr]
#19598447 - 02/21/14 07:33 AM (9 years, 11 months ago) |
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the price of pasty's original containers are enough to switch from disposable petris tho..u don't necessarily need dollar store kind. its the reusability that makes them great. those smaller ones may be useful for a print to agar or fresh tissue to agar, but it will be difficult to get culture to grow large enough to see sectoring and transfer from it if u are going for an isolate IME. u want enough growing room for the culture to tell u where it is most viable.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
Edited by blindingleaf (02/21/14 08:33 AM)
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synista



Registered: 08/21/11
Posts: 223
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Re: Pasty Agar Tek [Re: Valyr]
#19598706 - 02/21/14 09:18 AM (9 years, 11 months ago) |
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That makes sense. As you said, I see it could be useful for transfers or just culture storage, but no isolating.
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Delection
Spiritual Vagabond


Registered: 10/08/11
Posts: 848
Loc:
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Re: Pasty Agar Tek [Re: synista]
#19598841 - 02/21/14 09:54 AM (9 years, 11 months ago) |
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I can confirm those little containers are too small for isolating, but work great for culture storage.
-------------------- Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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ayemoner


Registered: 07/17/10
Posts: 225
Last seen: 9 years, 5 months
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Thanks for the info i will have to give these a try! I like the clear vision you get.
-------------------- **MY TRADE LIST**
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: ayemoner]
#19641553 - 03/02/14 08:51 PM (9 years, 10 months ago) |
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Quote:
ayemoner said: Thanks for the info i will have to give these a try! I like the clear vision you get.
Hope you enjoy
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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Forgot to post this but this is an 'entire plate' of galindoi culture I dumped in to rye berries that has begun to colonize out. The whole plate was fully colonized and it was easy to just shake it loose and drop the entire thing in. Minimal amount of time of the jar and the plate being open.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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That's one way to get a shitload of inoculate into ya spawn
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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Quote:
Pastywhyte said:

That's one way to get a shitload of inoculate into ya spawn 
Yes and for new comers who are afraid of scalpel work it's a good way to get into agar quickly while taking out some of the 'risk'. Though really if you can do this quickly enough without contamination then adding a few seconds of cutting probably isn't a problem either.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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BEHOLD!
 I found a 3P5 tucked behind a pile of tyvek sleeves. It's a clone from the first flush of my fiji pump tub. I guess I forgot it was there.  Just thought this pic would fit nicely here. PS. I move we now call these P35's or 3P5's or something [Pastywhite's PP - 5 tek] It's only 3 characters plus it sounds like it could be the name of a small gun that might go off accidentally and blow a hole in your crotch.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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Re: Pasty Agar Tek [Re: cronicr]
#19674375 - 03/10/14 12:40 AM (9 years, 10 months ago) |
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Quote:
cronicr said:

Good to see you're still here even if I'm not always. Also my full plate inoc galindois are colonizing nicely. I shook the shit out of the jars and there is mycelium growing from EVERWHERE OMG.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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