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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#21747670 - 06/01/15 08:38 AM (8 years, 7 months ago) |
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I'll take a couple pics tonight when I transfer and inoculate a jar. my last jars got contaminants and i'm not sure if its the tek I used to prepare my rye berries. I didnt soak my berries, I just added the ratio of water and berries and pc for the time specified. I think it was an hour and half. i'll double check before i do it tonight. I'm looking at doing my berries and agar at the same time in the pc, I heard the agar can take the added pressure cooking time.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Pasty Agar Tek [Re: Grey]
#21747685 - 06/01/15 08:47 AM (8 years, 7 months ago) |
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Quote:
Grey said: Agreed. They don't leave much condensation and they stack well.

What lids are you using on those half pints? I've been using metal grain jar lids upside down, but they're all rusted to hell now.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Where do you get half pints like those, do they have a name?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 23 days
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Re: Pasty Agar Tek [Re: abductee]
#21747716 - 06/01/15 09:01 AM (8 years, 7 months ago) |
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Same jars you use for pf tek, the lids are plastic, get em from Wal-Mart or basically anywhere they sell canning supplies
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: abductee]
#21747728 - 06/01/15 09:07 AM (8 years, 7 months ago) |
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Those jars are the ball 4oz baby food jars and come in packs of 6. The 4oz jars also come in packs of 12 as quilted jelly jars and packs of 4 as herb jars. They are all easily obtainable through most walmarts.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Yeah, for a short time Wal-Mart sold them just like this in four packs as storage jars. I haven't seen them again. The herb jars are still carried, same thing.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#21747758 - 06/01/15 09:22 AM (8 years, 7 months ago) |
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thanks guys, I just came back from getting some half pints and some litter jars, maybe the litter jars is an overkill, its probably better to use more 500ml than a couple litter jars, for faster mycelium growth. i'm going to use this tek but use half pint jars instead of the glass mini rounds this time around for the agar.
edited. I did some more reading and the quart size is good.
Edited by abductee (06/01/15 02:13 PM)
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: abductee]
#21748084 - 06/01/15 11:45 AM (8 years, 7 months ago) |
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here's some pics at 7 days, there may be duplicates of same plate but different angle.




there's a lot of condensation
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paperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
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Re: Pasty Agar Tek [Re: abductee]
#21749384 - 06/01/15 04:53 PM (8 years, 7 months ago) |
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I had a bunch set at an angle so popped them in the microwave to resolidify them. Afterwards I ended up with a layer of water on the top probably from condensation..
Just took tissue for a couple of clones and dumped the excess moisture out while I had the agar plates open in my SAB. Should work out all right.
-------------------- Why should we strive with cynic frown To knock their fairy castles down? ~ Eliza Cook It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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what i'm wondering about is what is growing, could I cut wedges out of any of it and transfer it to grain or another plate?
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Pasty Agar Tek [Re: abductee]
#21749528 - 06/01/15 05:36 PM (8 years, 7 months ago) |
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It's really hard to see to be honest. how did you inoculate them? Did you by chance squirt more than a drop of spores on the plate?
Does everything look fuzzy and white, meaning no different colors or milky blobs on the surface?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#21749551 - 06/01/15 05:40 PM (8 years, 7 months ago) |
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Those actually don't look that bad for condensation, I have seen worse with pour agar. That's the good news. The bad news is that the first two look like bacteria farms
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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sigh.. :/ disappointing that the one on top looks like a bacteria farm. those four pics are of two plates at different sides. It was hard to get pictures in focus with my camera. I choose those pics because they seemed to have the most growth. I tried to put three drops in each, kinda like a triangle.I noticed my first drop was probably a couple drops and the condensation on the agar caused it to kinda roll, but i quickly got the hang of getting single drops. there are plats that have blotches in different areas ( probably due to the liquid rolling,on second thought those could be the inoculation points. I was also holding the plates on a bit of an angle to inoculate or while setting it aside. maybe slightly bigger sab is in order) the blotchie ones do seem to have some milky blotches. I didn't notice any branching out or dikaryotic growth. I appreciate your input and expertise. I am continuing to look for information, there's so much on this site.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Re: Pasty Agar Tek [Re: abductee]
#21749893 - 06/01/15 07:01 PM (8 years, 7 months ago) |
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Quote:
abductee said: sigh.. :/ disappointing that the one on top looks like a bacteria farm. those four pics are of two plates at different sides. It was hard to get pictures in focus with my camera. I choose those pics because they seemed to have the most growth. I tried to put three drops in each, kinda like a triangle.I noticed my first drop was probably a couple drops and the condensation on the agar caused it to kinda roll, but i quickly got the hang of getting single drops. there are plats that have blotches in different areas ( probably due to the liquid rolling,on second thought those could be the inoculation points. I was also holding the plates on a bit of an angle to inoculate or while setting it aside. maybe slightly bigger sab is in order) the blotchie ones do seem to have some milky blotches. I didn't notice any branching out or dikaryotic growth. I appreciate your input and expertise. I am continuing to look for information, there's so much on this site.
While it's definitely possible to start cultures on agar with syringes, I have found that syringes just seem to be better used for PF tek. If you can grow just a couple decent looking fruits on a little cake (usually a 1 month process from inoculation) then you can take your own clean spore prints. Is this your first growing attempt or just your first try at agar?
Edit: Wait is this an LC you squirted on agar? I was reading your earlier posts and I'm unclear if your testing your LC or a spore syringe.
Your LC could be contaminated if that's what you're using.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
Edited by elasticaltiger (06/01/15 07:08 PM)
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 3 days
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Keep readying. This site is a treasure trove of invaluable resources. I lurked for over a year before posting much of anything.
Pasty plates are a wonderful way to start agar, cheap and effective. When I started with them I also ended up getting bacteria from syringes and poor technique. I didn't like that the plastic walls made it very difficult to see the culture clearly so I ended up switching over to pouring my own petri dishes.
I'd recommend you give it a try when you get some extra cash, a sleeve of dishes with shipping should be under 10 bucks and ebay has cases for 50-75. Since you already have agar you'd only need a container to pour from. I have a 500ml plastic erlenmeyer flask that I really like, going to order a 1000ml soon enough. I started out using a crown royal bottle 
I use parafilm to wrap my dishes but you don't have to if they're in a clean area for only a small amount of time. You could also use cling wrap(pretty sure).
Good luck on the agar work my man
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Credit where credit is due.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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no it was my mistake, it's a spore syringe. This is my first attempted at growing ( meaning forth attempt without contams), and my first agar.lol trying to pop all my cherries at once it seems.lol I'll have to check the glossary of terms in a moment to see whats the difference between liquid culture and spore syringe.
I got this syringe from a local head shop, the instructions were for oyster, but the word panama were written on the top, this was a photocopy instruction with photocopy panama writing on top. ( he didnt just write panama in pen) and dont see why he would buy an oyster syringe. so i'm down to 2cc's I think i'll just inoculate my rye, but let rinse the rye, soak, boil, steam.. pc. Im going to use something for a filter. I saw on a video the synthetic insides of a pillow is like poly fil.. so i'll use that and be sterile again and just hope my rye colognizes. This doesnt end here though. I'm kinda set on using rye berries, its what I can find and its good to inoculate other jars with. Sorry I kinda took advantage of this thread, thanks for the help.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Pasty Agar Tek [Re: abductee]
#21750013 - 06/01/15 07:24 PM (8 years, 7 months ago) |
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If you can get some mycelium growth on grains and it gets a contam you can still grab a colonized grain that looks healthy and drop it in agar to try and clean it up. I'm about to do that with this bacterial Ovoid print I have.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#21750023 - 06/01/15 07:26 PM (8 years, 7 months ago) |
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REally? There were quite a few nice grains, little clumps that seemed perfectly white.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Re: Pasty Agar Tek [Re: abductee]
#21750032 - 06/01/15 07:27 PM (8 years, 7 months ago) |
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Quote:
abductee said: Sorry I kinda took advantage of this thread, thanks for the help.
That's what we're here for.
By any chance could we see your syringe and booklet? Just a quick picture?
If you bought it in a head shop there is no telling how old the syringe is or how it was stored. Even if it was in a ziplock bag or something if it was kept at room temperature for a very long time there may be a little bit of bacteria floating around in there (doesn't mean all is lost though.)
You got moxy diving into agar first thing. I like that.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Tiger is right. It might be a steeper curve and take a little longer but a year from now you will be miles ahead. This hobby ain't a sprint.
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