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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD]
    #21532271 - 04/11/15 10:08 AM (8 years, 9 months ago)

I have done both. I like having the bottom wrapped because when I crush it down it makes a nice stable platform to prevent it from shifting.  I use a giant roll of foil and it does hundreds of plates. But its really up to what people feel like doing.


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OfflineOrganic_Magic
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD]
    #21532280 - 04/11/15 10:10 AM (8 years, 9 months ago)

I use the paper towel and then a square of foil just big enough to wrap and crimp under the lid. I can get them to sit level like that.


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:pipesmoke:Its all fun and games until mushrooms make you gay:pipesmoke:




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Invisiblewowimflabbergasted
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Re: Pastywhyte's Easy Agar Tek [Re: Organic_Magic]
    #21532301 - 04/11/15 10:14 AM (8 years, 9 months ago)

I'll have to try that pasty.


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OfflineEdmunter
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Re: Pastywhyte's Easy Agar Tek [Re: wowimflabbergasted]
    #21532783 - 04/11/15 12:15 PM (8 years, 9 months ago)

So Muda I couud st put thos psi mexs on grains even though they are slow and small.  I owe half the world prints and they are sending the mushroom baliffs round soon so I need some grows.


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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: Edmunter]
    #21533526 - 04/11/15 02:21 PM (8 years, 9 months ago)

I made an LI using growth that was only about 3/4" in diameter. I used it to inoculate 7 bottle subs and 2 grain jars with a full 60cc syringe left over. They are colonising just fine.


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AMU
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Invisibleswatt_haze
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Re: Pastywhyte's Easy Agar Tek [Re: spacechildo]
    #21533860 - 04/11/15 03:41 PM (8 years, 9 months ago)

Quote:

spacechildo said:
Pasty, do you still wrap your entire rounds in alu foil or just a cap over the lid?
Or anyone else?




I just read his tek and i wrapped the whole thing. I was confused on this aswell? I got em in the PC right now.  NO MORE CONTAMS!


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Invisiblestareatclouds
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Re: Pastywhyte's Easy Agar Tek [Re: swatt_haze]
    #21533900 - 04/11/15 03:51 PM (8 years, 9 months ago)

I swear I posted this earlier, but whatever. Anyway, I just use enough foil to cover the lid and keep the top dry, it works for me. I like the paper towels because I can recycle them every time and they're very easy to keep writing the species/variety on. A Sharpie will black out the last writing and it's free to mark again for the next batch.

I also took some out after PC, let them cool in SAB, and forgot about them for like a month. I didn't refrigerate or anything and when I used them a month or so later, they were fine. Not saying that's optimal, but storing blank ones at room temperature didn't mess anything up for me.

Also want to confirm that generic "waterproof surgical tape" from grocery store worked just fine as I could never find micropore tape.


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Invisibleswatt_haze
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #21533919 - 04/11/15 03:57 PM (8 years, 9 months ago)

Quote:

stareatclouds said:

Also want to confirm that generic "waterproof surgical tape" from grocery store worked just fine as I could never find micropore tape.




Yea i just bought micropore tape online and when i get it its practically the same thing.  My moms a nurse and i spent 3.99 on a roll when she has hundreds of rolls...


Edited by swatt_haze (04/11/15 03:57 PM)


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OfflineDirdyD
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #21534088 - 04/11/15 04:48 PM (8 years, 9 months ago)

Yaknow, I've always wondered about that!
Would've saved me a bunch of time and elbow grease.

Thanks, Star.


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InvisibleTheEaglesGift
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD]
    #21534171 - 04/11/15 05:20 PM (8 years, 9 months ago)

If you're using micropore tape for GE holes on pasty plates, I suggest doubling or even tripling up the layers.

If you look at micropore tape under a microscoep, it's a little discerning how large the holes in it are. Very large.


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OfflineDirdyD
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21534179 - 04/11/15 05:22 PM (8 years, 9 months ago)

Right on, Eagle.


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InvisibleTheEaglesGift
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD]
    #21534255 - 04/11/15 05:46 PM (8 years, 9 months ago)

You could even use 4 or 5 layers of tape and still get plenty of gas exchange. Better safe than sorry. I don't trust that tape. I don't use it alone anymore.


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InvisiblebodhisattaMDiscordReddit
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21534260 - 04/11/15 05:47 PM (8 years, 9 months ago)

stacking filters doesn't make them more effective. 1 or 3 or 5 it's still only good to the pore size


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InvisibleTheEaglesGift
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Re: Pastywhyte's Easy Agar Tek [Re: bodhisatta]
    #21534263 - 04/11/15 05:48 PM (8 years, 9 months ago)

Ever look at 5 pieces of micropore tape under a microscope compared to 1? It makes a difference man.


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InvisiblebodhisattaMDiscordReddit
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21534277 - 04/11/15 05:51 PM (8 years, 9 months ago)

nope. and yes I know exactly what it looks like.

stacking filters doesn't increase their micron rating.

the way micropore works though is like the dry verm barrier. though they're both shit filters you rely on the fact there's never pressure going across the filter just diffusion. if you had to suck air through it 1 layer or 100 layers wouldn't make the slightest.


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InvisibleTheEaglesGift
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Re: Pastywhyte's Easy Agar Tek [Re: bodhisatta]
    #21534806 - 04/11/15 08:13 PM (8 years, 9 months ago)

You're not "stacking" filters you're actually increasing the ply of the material. If you put 5 layers down it's going to be harder for a mold spore to make it through 5 layers rather than 1 layer, no?


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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21535110 - 04/11/15 09:52 PM (8 years, 9 months ago)

The more layers you use the more restricted the air flow is so I don't see how it wouldn't keep contams out better. SFDs are made of multiple layers of filter paper. If adding more layers didn't increase the filters effectiveness. SFDs wouldn't work any better than a single layer of filter paper.


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AMU
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InvisiblebodhisattaMDiscordReddit
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21535263 - 04/11/15 10:31 PM (8 years, 9 months ago)

Quote:

TheEaglesGift said:
You're not "stacking" filters you're actually increasing the ply of the material. If you put 5 layers down it's going to be harder for a mold spore to make it through 5 layers rather than 1 layer, no?



nope otherwise there wouldn't be a need for sterile filters.
SFDs are stacked yes but they're stacked in a way that ensures a specific micron size. you can put 30 of those blue fiberglass air furnace filters in a row and you're not going to make a flow hood.


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InvisibleTheEaglesGift
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #21535423 - 04/11/15 11:36 PM (8 years, 9 months ago)

Quote:

MudaFuka said:
The more layers you use the more restricted the air flow is so I don't see how it wouldn't keep contams out better. SFDs are made of multiple layers of filter paper. If adding more layers didn't increase the filters effectiveness. SFDs wouldn't work any better than a single layer of filter paper.



At least I'm not totally in left field with my way of thinking. Maybe it's just you and me Muda, out in left field...stacking filters...being cray cray.


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OfflineDirdyD
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Re: Pastywhyte's Easy Agar Tek [Re: TheEaglesGift]
    #21537633 - 04/12/15 02:28 PM (8 years, 9 months ago)

Question..

Is it safe to open a pp5 that is 95-100% colonized to inspect/photograph? 

It's difficult to see through these blue Glad tops.


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