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 blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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interesting jj, def let us know if it works (in another thread). I'm also down to try!
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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elasticaltiger
Like Tigers in Coitus
Registered: 06/24/13
Posts: 8,438
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I don't know if anyone else has mentioned this yet, but I love how you can just slide the whole puddle out into your grain jar instead of messing around with cutting (unless you want to of course.)
It makes for really easy grain inoculation. Sure you could get four jars with one but it's so darn easy to just drop the whole thing in and call it good. The size of the puddle is basically the same as a 'third' of a regular petri. 
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First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it  Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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Pastywhyte 
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Shit man I never tried that. Gonna give it a shot 
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 Forrester
aspiring sociopath
Registered: 02/05/13
Posts: 9,352
Loc: Northeast USA
Last seen: 1 month, 11 days
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Quote:
elasticaltiger said:
I don't know if anyone else has mentioned this yet, but I love how you can just slide the whole puddle out into your grain jar instead of messing around with cutting (unless you want to of course.)
It makes for really easy grain inoculation. Sure you could get four jars with one but it's so darn easy to just drop the whole thing in and call it good. The size of the puddle is basically the same as a 'third' of a regular petri.
It's so dry here, mine crack into pieces within a day after cutting my first wedge anyway. So there's a bunch of wedges, handy!
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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Quote:
*** About six milliliters of sterile distilled water is pipetted aseptically into a freshly growing culture. The fragments of hyphae are dislodged by lightly scraping the aerial growth with the same pipette, and the resulting suspension is withdrawn and transferred to a sterile glass vial. Put plenty of inoculum into each vial to insure success.
Sounds like they were knocking mycelium of the "freshly growing culture" that was perhaps on grains (knocked into water) and transferring them to a sterile glass vial
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elasticaltiger
Like Tigers in Coitus
Registered: 06/24/13
Posts: 8,438
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If you're going to dump the whole plate into the jar I would suggest these things:
1) Make sure full colonization is pretty much complete (wait a day after full colonization just in case.)
2) squeeze the bottom of the containers before you open them. This will cause the puddle to 'jump' out of it's wedge a little. From there you can just shake it around and drop it.
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First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea i def gotta try that. its strange to hear "full colonization" in relation to petris.
--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Quote:
blindingleaf said:
yea i def gotta try that. its strange to hear "full colonization" in relation to petris.
That's because it's poor practice to let it crawl all the way to the outer edges of the dish, that is where contaminants tend to slip in, if they are going to.
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Delection
Spiritual Vagabond
Registered: 10/08/11
Posts: 848
Loc: 
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Hey FrankHorrigan whats with the block Anne H. in your signature? Whats that all about?
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Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Quote:
Delection said:
Hey FrankHorrigan whats with the block Anne H. in your signature? Whats that all about?
I could UTFSE but I'm betting you have some stunning examples off the top of your head.
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tripmob
strang and unusual
Registered: 01/04/14
Posts: 176
Last seen: 7 years, 6 months
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 Re: Pasty Agar Tek  [Re: Inocuole]
#19481250 - 01/27/14 01:05 PM (10 years, 2 months ago) |
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Meh, anna and her associate are firm supporters of vtek grows, they respond very....erm....passionatly... to anyone who questions the superiority of vtek. As you can imagine, this has lead to some very heated threads. Traditional bulk growers vrs. Vtekers.
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Theres only 2 things I hate in life, Racism and Chinese People.
10, 000 rocks of crack cocaine is too much!
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
FrankHorrigan said:
Quote:
blindingleaf said:
yea i def gotta try that. its strange to hear "full colonization" in relation to petris.
That's because it's poor practice to let it crawl all the way to the outer edges of the dish, that is where contaminants tend to slip in, if they are going to.
yea i know, thats why its weird to hear
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Quote:
blindingleaf said:
Quote:
FrankHorrigan said:
Quote:
blindingleaf said:
yea i def gotta try that. its strange to hear "full colonization" in relation to petris.
That's because it's poor practice to let it crawl all the way to the outer edges of the dish, that is where contaminants tend to slip in, if they are going to.
yea i know, thats why its weird to hear
As someone with no agar experience who's looking to get into it soon, can I ask some clarification?
You're saying it'd typically be a bad idea to let your culture grow out to the edges of the dish? Wouldn't it be better for it to be colonized than for it to be bare if contaminants are the concern? If not, how would you stop it from growing past where you want it to stay? Not sure if I fully understand.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Pasty Agar Tek [Re: Inocuole]
#19482248 - 01/27/14 04:37 PM (10 years, 2 months ago) |
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thats when you either transfer or move to a slant and put it in a fridge,preferably not your food fridge there filthy(or you can just put the pastyplate in the fridge)
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   It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: cronicr]
#19482586 - 01/27/14 05:55 PM (10 years, 2 months ago) |
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and what Frank said (we need a :whatFranksaid: smiley)
the edges of agar plate are most prone to contam because they are closest to the outside environment (spores, mold, etc) so the closer the myc gets to that environment, the more likely to contam it can be. if u don't have slants (i do not...yet) i just put them in fridge when they r grown out to this size:
that way, if a contam did enter near plates edge, u will be able to see it on the agar. if the myc grows to the edge, u may not b able to see it, and it will hitch a ride with the wedge u take into ur grain jar/plate/bag, etc.
u don't need parafilm for the pasty tek, but i still wrap them in saran wrap/cling wrap before putting them in fridge, or u can use plastic bags.
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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winglang
bon bon
Registered: 12/03/13
Posts: 3,021
Loc: Big Green Amore
Last seen: 10 days, 20 hours
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I use a 1 1/2" stir bar in a quart mason jar to
stir my agar and malt extract at room temp. I turn it up as much as
possible, to the point just before the stir bar gets kicked off
(substantial vortex). Everything is dissolved nicely  . Anybody else
do this?
On second thought, perhaps it's tougher to dissolve different grades of
agar. I'm using some microbiology grade agar.
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J. Jack Flash
stranger than ever.
Registered: 11/20/13
Posts: 1,500
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Re: Pasty Agar Tek [Re: winglang]
#19542300 - 02/09/14 12:46 AM (10 years, 2 months ago) |
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oh fuck off, you. this tek is about using lidded plastic shot glasses for culture plates, and you chime in with microbiology grade stir plate nonsense. ... i love ya like i love e'rybody but really?
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the j stands for jesus.2020 new years grow along
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winglang
bon bon
Registered: 12/03/13
Posts: 3,021
Loc: Big Green Amore
Last seen: 10 days, 20 hours
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Quote:
J. Jack Flash said:
oh fuck off, you. this tek is about using lidded plastic shot glasses for culture plates, and you chime in with microbiology grade stir plate nonsense. ... i love ya like i love e'rybody but really?
I think you misunderstood me. I have microbiology grade AGAR
from ebay.. My stir plate is cheap; its made from a computer fan
and magnets. I think that some people who use this tek may also have a
stir plate. I thought the point of this thread was making agar
easier?!?!
So...it takes me one minute to dissolve everything (lme and agar) in a jar
vs. however many minutes it takes on the stove. Also, in this thread,
there was mention of letting the agar cool back down after dissolving.
Using a stir plate, could cut out a few steps. I, for one, like less
steps.
edit:just realized its molecular biology grade agar
http://www.teknova.com/product-p/a7774.htm[/strike]
After trying again it doesn't really dissolve, it just mixes well. Stove top with heat works better.
Edited by winglang (03/09/14 09:02 PM)
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J. Jack Flash
stranger than ever.
Registered: 11/20/13
Posts: 1,500
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Re: Pasty Agar Tek [Re: winglang]
#19542763 - 02/09/14 07:25 AM (10 years, 2 months ago) |
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you are correct, i did misunderstand. apologies. i'm surprised to hear stirring alone is enough to make agar work. i thought heat was required, like gelatin.
for that matter, it sounds like one could put it in a blender. well anyway, a half liter of water boils quick and the pan is easier to clean.
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the j stands for jesus.2020 new years grow along
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BittrBuffalo
Deaconica
Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 7 months
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Quote:
blindingleaf said:
Quote:
I think your right. Lots of times my worst condensation would happen when I shut the stove off and took it of the burner. Now I drop the heat slow and leave the pc on the burner to cool off slow. I rarely have condensation issues.
ok will try this next time.
no thoughts on the paper towel?
I tried the screw-top containers for agar plates, and that didn't work out awesomely in terms of condensation. I got a LOT of it. Anyway, what I did to wipe out the condensation was turn the containers upside down and let them sit in the SAB for a minute. Then I tilted them on their sides and cracked them about a 1/4 turn to let the water drain out, then opened them gently and used individually wrapped sterile gauze to wipe out the sides and lid. I used one for every dish. This time I'm using the Pasty plate method to the letter with the mini rounds. 
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Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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