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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: cronicr]
#19453102 - 01/21/14 02:04 PM (10 years, 8 days ago) |
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yea thats why i wait to take them out. its hard to see in "high def" with these in the first place compared to regular plastic petris, but with extra condensation, even more so.
yo cron, u should change ur title to "a thread without the cron is like a beat without a snare"
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: cronicr]
#19456280 - 01/22/14 06:05 AM (10 years, 7 days ago) |
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pasty and other who use this tek, thought i'd tell u what i tried tonight, chime in and either me out cause it was dumb, or me on cause it just may work!
so with this tek, the only consistent draw back i notice from multiple users of it, and other no pour teks like it, is the condensation. this can be slightly remedied by letting agar cool after pouring, before PC'ing. this cycle, mine cooled over 9 hours before pc'ing, there was NO moisture, condensation on walls or lids of the PP5. after pc cycle, i let it cool for over 12 hours. cool to the touch, not even warm. took them out, CONDENSATION everywhere WTF!! this does not happen all the time, but this time i thought for sure because i waited before pc and after pc cool down way more than i usually do that there would be no condensation.
so what i did, while working in the SAB doing transfers, is after i take the foil off, i use the paper towel on top of lid (also sterile at this point) and wiped the walls of the container before dropping wedges in. obviously, if u do a cycle with a lot of these plates, u may not use ALL of them right outta the pc, so those put aside for a later date, will probably not still have a convenient sterilized paper towel to use for this (or do u think it would still b sterile, as it was wrapped in foil entire time?).
i will let u guys know if it works. whatd'ya think? u can call me crazy, i don't mind. i did this once with a cotton ball (not even sterile, straight outta the bag) and a regular petri and it worked a while back (but it was one time, one plate, not conclusive enough), so i figured why not try it again with these because i use this tek a lot.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
Posts: 24,863
Loc: ★
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Unrelated to the topic, but I saw your subtext "I use parentheses a lot" and then I started reading your post and was like "hmm, yes, I agree." Got a good chuckle out of that sequence.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: Inocuole]
#19456445 - 01/22/14 07:42 AM (10 years, 7 days ago) |
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, i tend to have interjecting thoughts as i post, and they make more sense in () than adding another sentence. i hope my posts make sense even with them. of couse when i read them they do, but dunno if others are just like "WTF?? fuck that parenthetical noise!!" haha
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: cronicr]
#19456706 - 01/22/14 09:35 AM (10 years, 7 days ago) |
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Quote:
cronicr said: condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away
I think your right. Lots of times my worst condensation would happen when I shut the stove off and took it of the burner. Now I drop the heat slow and leave the pc on the burner to cool off slow. I rarely have condensation issues.
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HypnotoadCroaked
Retired, but will check MSGs


Registered: 01/05/13
Posts: 1,168
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Re: Pasty Agar Tek [Re: cronicr]
#19456751 - 01/22/14 09:48 AM (10 years, 7 days ago) |
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Quote:
cronicr said: condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away
I think:
Condensation will happen because of the temp differential that easily can exist with a thin plastic container. No matter how slow or fast you cool your PC/Autoclave, as it cools the material inside of the jar will ALWAYS be warmer than the surrounding environment. I feel that this is the best explanation of the condensation issue.
I am thinking that cooling off the PC slowly may reduce the amount, but as chronicr states, it will never totally alleviate. Another issue with this idea is that a slow cool off is still going to be HOT and cooking the agar....I don't know how this would affect overall cook time. Do you change to 30min @ 15, and 2 hours of cooling down from 250f to room temp? I doubt it matters, but just a thought.
Glass jars experience much less because of the glass being a HORRIBLE conductor of heat. By the logic that glass takes longer to heat, it also will take much longer to cool versus the plastic.
Just a theory based on the ease of conductivity of heat from outside the containers to the inside.
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 9 hours, 18 minutes
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what do all you no-pour tekkies use for culture slants when you want to save some genetics? do you keep master cultures in no-pour agar rounds? do you actually pour agar into slants sometimes?
I'm thinking of using no-pour rounds as master culture jars...
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: Generic]
#19457001 - 01/22/14 10:43 AM (10 years, 7 days ago) |
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I use slants for long term storage. Have not really found a substitute for slants.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 23 days, 23 hours
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Re: Pasty Agar Tek [Re: Generic]
#19457003 - 01/22/14 10:43 AM (10 years, 7 days ago) |
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Quote:
Generic said: what do all you no-pour tekkies use for culture slants when you want to save some genetics? do you keep master cultures in no-pour agar rounds? do you actually pour agar into slants sometimes?
I'm thinking of using no-pour rounds as master culture jars...
Master slants are already no pour, just PC the culture tubes with agar in them. Take them out before totally cooled and put them on a slant to finish cooling/solidify.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
I think your right. Lots of times my worst condensation would happen when I shut the stove off and took it of the burner. Now I drop the heat slow and leave the pc on the burner to cool off slow. I rarely have condensation issues.
ok will try this next time.
no thoughts on the paper towel?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
Pastywhyte said: Have not really found a substitute for slants.
There isn't one
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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http://www.shroomery.org/8509/Resurrecting-a-Better-Method-for-Long-Term-Storage-of-Mushroom-Cultures
Does this method not work for y'all? Reading about and trying it myself, i like this idea better because it seems like it'll last much longer and requires no refrigeration. Already have a couple vials from a multispore plate just to explore the process. The jars i have growing out from them are nearly 100% Anyway, anyone else use this method to store their strains?
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the j stands for jesus.2020 new years grow along
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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jm mcdoogle (i think that his poster name) posded a thread about that little bit back and many responded, some who have tried with success if i remember correctly. look at his ratings and someone links to it, i think amanita virosa
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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no contam yet on my "sterile paper towel" experiment.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
J. Jack Flash said: http://www.shroomery.org/8509/Resurrecting-a-Better-Method-for-Long-Term-Storage-of-Mushroom-Cultures
Does this method not work for y'all? Reading about and trying it myself, i like this idea better because it seems like it'll last much longer and requires no refrigeration. Already have a couple vials from a multispore plate just to explore the process. The jars i have growing out from them are nearly 100% Anyway, anyone else use this method to store their strains?
I gave that a cursory glance, are you asking if you can use water to store cultures? I think that would be called a grain LC
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Inocuole
Scalpel of Evil's Bane




Registered: 11/21/11
Posts: 24,863
Loc: ★
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"Myceliated water" is what RR likes to refer to it as, and I must respectfully agree, since they're not living on the water so much as hanging in stasis in it. I'm not sure however if that's any more likely to survive over a length of time VS tradtional LC, which is the only place I could see a downside in that method. There is that thread floating around about a year old "glc" being resurrected, so who knows, maybe I should've kept some of my old ones.
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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Re: Pasty Agar Tek [Re: Inocuole] 1
#19468999 - 01/24/14 06:54 PM (10 years, 5 days ago) |
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i think there's a distinction between that and liquid culture since there's zero nutrition in the liquid medium, and grain never enters into it. like you said, in stasis. i looked for the thread you mentioned, leaf, but i couldn't find. i'll try again later on. (pasty, i feel it's marginally on topic, but if you disagree, please advise) what i did probably sounds dumb, but i like it and so far it has been successful. using dropper dram vials such as these, i filled them with distilled water. PC'd for some amount to time, i forget off hand, to sterilize them. took two of them, and a fluffy multispore plate. one at a time in still air, squirted a few droppers full of water into the plate, scraped around with the dropper and slurped it all back up. rinsed, repeated. based on the work cited in the article, it should/could/would (might) remain viable for years, if stored in reasonable conditions. then just put a drop on agar to grow out for master inoculation and bickity bam, the motherfucker is rubble. (i love that 'motherfucker' wasn't marked as a misspelling) one drop at time, should last for at least as long as i'll be into growing, which i feel will be quite long, indeed. and when i'm done, they'll make great gifts.
of course, it's not worth the effort for the sake of multispore, but with isolates, i feel it shows real promise, without refrigeration, or worries about repeating the slant process every year or so.
in retrospect, yeah this is a clear , but i'm not wasting the above typing... sorry, y'all. you would not believe what a geek i am.
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the j stands for jesus.2020 new years grow along
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