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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Re: Pasty Agar Tek [Re: hamloaf]
#21189403 - 01/27/15 11:14 PM (9 years, 3 days ago) |
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Quote:
hamloaf said: You can do this type of no-pour agar tek with glass half pint wide mouthed, or those half pint squat jars that are wide mouthed, and regular grain jar lids. Been meaning to write that tek, but have been sidetracked with this whole grain water culturing technique phenomenon.
The later style of jar described above is preferred by this researcher due to the larger surface are of the bottom of the squat jars over the regular half pints standard averagely used with BRF cakes. More room for the myce to run.
I was doing it with 1/4 pint wide mouths, but as stated earlier, I somehow blew some green mold spores on my dish thus making me try this method as I can work on my dishes upside down / at least sideways and not rite side up.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Dude, think I'm going to write the tek. If anybody didn't write a tek because said tek has already been written, there would be no teks to write. I mean, hell, the baby bottle tek is nothing new either. In fact the baby bottle tek is old, and I'm pretty sure Doc_T wrote a no-pour agar tek. Beside, there's a small innovation to the no-pour agar tek to introduce that recycles a material that gets asked a lot about how to recycle said material.
I'll call it "hamloaf's advanced, Pain In The Ass, No-Pour Agar Tek".
Edited by liquidmyce (01/28/15 03:32 AM)
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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You guys should have a look at this thread. http://www.shroomery.org/forums/showflat.php/Number/16905126#16905126 Not taking away from Pastys tek but there are many ways to skin a cat. I've used this before and it has worked.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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u can fit way more pasty plates in PC cycles they are less heavy easier to maneuver in SAB, and can fit more in SAB easier to store almost as re usable dropping the entire plate into a wide mouth is always possible, they are perfect size
lots of advantages to these smaller pp5's IMO
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
hamloaf said: Dude, think I'm going to write the tek. If anybody didn't write a tek because said tek has already been written, there would be no teks to write. I mean, hell, the baby bottle tek is nothing new either. In fact the baby bottle tek is old, and I'm pretty sure Doc_T wrote a no-pour agar tek. Beside, there's a small innovation to the no-pour agar tek to introduce that recycles a material that gets asked a lot about how to recycle said material.
I'll call it "hamloaf's advanced, Pain In The Ass, No-Pour Agar Tek". 
I don't consider rewritten teks to be a negative thing at all. If the only change is better pictures its still a success. If a re written tek has even the slightest variation then its a breakthrough. I consider mine to be a rewrite of every no pour method already covered despite the fact that mine was the first to use these specific containers. Even an improved vernacular or better use of jargon means there is something to be learned.
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Violet



Registered: 12/06/11
Posts: 4,205
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Yeah, same here with my no-pour tek. Mine is little different (kindof why I had it at the beginning of a more inclusive tech) except accounts for a different container, which needs no modification. That and I specifically outlined the tek using the grain boil water like hamloaf is talking about. I don't do it with glass, for the same reason Muda says... pp5 is far superior. But I actually started no-pour agar with glass.
 Used to have several more photos of things like those... They must have dissappeared with tnh.com... oh well. Kinda nifty. You can reach in with a scalpel or screwdriver for wedges of course, but I also enjoyed injecting water, shaking it around, and aspirating it back for a quick mycelium water. In fact I did that with the jar in the photo, that may be why it recovered and fruited like that.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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Quote:
blindingleaf said: u can fit way more pasty plates in PC cycles they are less heavy easier to maneuver in SAB, and can fit more in SAB easier to store almost as re usable dropping the entire plate into a wide mouth is always possible, they are perfect size
lots of advantages to these smaller pp5's IMO
that's the thing they are roughly the same size but OP used rectangular pp5s about the size of a playing card and you only put a thin film of brf on the bottom of the container. Not PF tek jars. I actually used WBS flour to make mine. I'm off today so I might go buy a shitake or portobella since I have to pick up mini rounds anyways and my agar and print won't be here till Monday. I haven't grown anything in about 4 years so the practice won't hurt.
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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The silicone does not seem to adhere to well, I rubbed some spillage off the lid quite easily with my finger.
Maybe it will be fine, only time will tell.
It is wal mart silicone maybe I should have not gone to wal mart lol.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
Edited by budmanman (01/28/15 01:01 PM)
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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If you're having trouble with it sticking use sandpeper around the hole to rough up the lid. and let the silicon cure completely. the instructions usually say 8 hours but 24 works better.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Yah man. Any time you are using silicon on a surface with a smooth finish. You should sand it first.
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budmanman
OTD Masterbater



Registered: 02/07/07
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Loc: PNW
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Re: Pasty Agar Tek [Re: MudaFuka]
#21192849 - 01/28/15 05:47 PM (9 years, 2 days ago) |
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Well I will make sure to do that in the future, never had a issue with metal lids but I think silicone adheres to metal quite strong.
it seems to have sealed my only concern is it breaking free while pcing.
I think its should be fine though.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Silicone actually doesn't adhere to smooth metal lid as well as it dose when they are sanded. Sand your lids and your silicone job will last a lot longer.
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TheGuru
Doctor


Registered: 01/19/15
Posts: 77
Last seen: 8 years, 11 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#21193133 - 01/28/15 06:43 PM (9 years, 2 days ago) |
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I agree with the sanding. i sand when i use silicon to make my injector ports. usually when my store bought ports r used up n i forgot to order some. i wait 24 to 48 hours for the center to dry because the manufacturer cure time isnt for a 1/2 inch blob its for a thin bead
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: TheGuru]
#21204573 - 01/30/15 09:26 PM (9 years, 13 hours ago) |
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This is the one Burma plate I've gotten to colonize with anything after like 6-7 tries. I dropped a few droplets on it. Is this just completely fucked or worth transferring a bit of the edges to new plates?
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budmanman
OTD Masterbater



Registered: 02/07/07
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Loc: PNW
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I am attempting to save this one my self lol.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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hamloaf
Loaf of Fam.

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Quote:
stareatclouds said: This is the one Burma plate I've gotten to colonize with anything after like 6-7 tries. I dropped a few droplets on it. Is this just completely fucked or worth transferring a bit of the edges to new plates?

Looks good to me. Since you used several drops of spore solution what you have going on there are just multiple sights of germination. I get that ALL the time when I transfer LC to agar, OR if I put a wedge on a plate and the wedge moves around.
Use an inoculation loop to inoculate spores to agar.
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Cl0ud.9.Warl0ck
Hunter



Registered: 05/17/14
Posts: 484
Loc: South East
Last seen: 8 months, 3 days
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Quote:
stareatclouds said: This is the one Burma plate I've gotten to colonize with anything after like 6-7 tries. I dropped a few droplets on it. Is this just completely fucked or worth transferring a bit of the edges to new plates?

It is red. What is your method friend?
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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Quote:
Cl0ud.9.Warl0ck said:
Quote:
stareatclouds said: This is the one Burma plate I've gotten to colonize with anything after like 6-7 tries. I dropped a few droplets on it. Is this just completely fucked or worth transferring a bit of the edges to new plates?

It is red. What is your method friend? 
he used colored agar or added food coloring. It's on the first page of the thread.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Quote:
It is red. What is your method friend?

-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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r00tuuu123
Now I'm just really piseed



Registered: 04/20/12
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Just did my first batch. In PC cooling now. I made a few tweaks to fit what I had to work with I used boil water instead of potato flakes and karo. And had to use these since the store was out of the rounds. http://www.ziploc.com/Products/Pages/ContainersOnePressSeal.aspx And this was very helpful since I was only doing 12 containers. http://www.hometrainingtools.com/agar-6-g-dehydrated/p/CH-AGAR/ I just doubled the recipe listed an the ammount worked out just right. But before that I ran 8 cups of grain water through the coffee maker to remove some of the solids and poured off 200 ml into a pkastic measuring cup added 2 level TSP of agar and stired it in added 2 drops of blue food coloring. Then just poured my containers and let it set up for about 1/2 hour (came out a bit softer than I expected maybe just a smidge more agar next time) Find out tomorrow when I Noc em up. All in all pretty simple
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