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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: MudaFuka]
#19428594 - 01/16/14 03:11 PM (10 years, 13 days ago) |
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Well I have used the screwtops and didn't like them. They are not nearly as easy to manipulate in the SAB, I can hold and open my minis with one hand.Also I don't like the depth of the ziplocks too easy to touch the rim with my scalpel unless I reach over the mouth. Plus if you are going to G2G how many masters do you really need? 1 quart can easily be expanded to1000 by the third expansion personaly I'm not a fan of cutting more than a few wedges from single plate anyway. Might feel differently if I had a hood, but I don't.
This is not a contest. If someone prefers ziplocks or jars or pour agar cool. As long as there doing agar I'm happy. But if someone tries agar and has a hard time with one method or another container, this is another option for them to try before they give up on it.
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Valyr
Con nulla non si fa nulla.


Registered: 05/08/13
Posts: 381
Last seen: 6 years, 9 months
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Personally Pasty, if I hadnt of found this tek I would still be waiting for a large flowhood and petri's etc instead of having everything I need minus a flush from my current tub so I can take a clone .
Its value in that alone, getting peeps to jump into agar earlier is priceless. The simplicity of the tek and readily available materials really opened my mind to the possibilities when previously I wasnt even entertaining the thought. Thanks for doing it.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Pasty Agar Tek [Re: Valyr] 1
#19428739 - 01/16/14 03:36 PM (10 years, 13 days ago) |
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You just need a SAB to work with petris and agar
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Valyr
Con nulla non si fa nulla.


Registered: 05/08/13
Posts: 381
Last seen: 6 years, 9 months
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Yeah but it was pasty's tek that made it dawn on me. I know it should have all on its own but for some reason I was just stuck in that mental wall of cant do it without.... blah blah blah
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: Valyr] 1
#19428947 - 01/16/14 04:15 PM (10 years, 13 days ago) |
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Sometimes we just can't see the forest If I can get just one person to start using agar with this writeup, it has done its job.
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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I have been using the twisties, which are huge by comparison to the minis, but i feel they stack better. I have had some instances nesting them in the cooker where they seal between each other when they cool and deform from the vacuum created. I figure using foil or something to make a gap will solve this. And i agree, they're a bitch in the still air box, but so far, so is most everything else.
I bought some minis, and think the lids can be cracked for sterilizing, so the lid mods wouldn't be necessary. Haven't tested this idea, tho. Based on my experience with the twisties, i'm convinced gas exchange isn't necessary.
My current thinking is the twisties are better for growing out for isolating sectors, while the minis are better for growing noc wedges. An entire mini plate can be dropped in a jar and shaken up. RR dropped entire thirds from y plates into grain jars, gave me the idea.
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the j stands for jesus.2020 new years grow along
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea u can def drop a whole snap lids worth of agar into a grain jar (cut it up first though). i tried the pasty tek with no tin foil, water got thru the micropore. when i tried it with foil covering the cracked lid and no hole, water still got in. that hole is not for GE in this TEK, its meant for gas to escape when the container is actually cooking (correct me if I'm wrong though pasty). if u have luck with no lid mod, let me know. if u wrap the entire container with foil (like the OP tek says) u can probably get away with a cracked, no hole lid. but thats a lot of foil. post results if it works!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Quote:
blindingleaf said: yea u can def drop a whole snap lids worth of agar into a grain jar (cut it up first though). i tried the pasty tek with no tin foil, water got thru the micropore. when i tried it with foil covering the cracked lid and no hole, water still got in. that hole is not for GE in this TEK, its meant for gas to escape when the container is actually cooking (correct me if I'm wrong though pasty). if u have luck with no lid mod, let me know. if u wrap the entire container with foil (like the OP tek says) u can probably get away with a cracked, no hole lid. but thats a lot of foil. post results if it works!
Your right, the hole is not for GE its to prevent a vacuum from crushing the container. I always buy a massive roll from costco for cheap, so using a square per container never really bothered me. Since I use SFD for my jar lids now I don't really use much foil so a roll lasts for a more than a hundred plates or so. You could use SFD for these, but really a few squares of micropore and some foil does not really bother me much. Plus I like the fact that once you unwrap them, the container itself is relatively sterile, one less thing to sanitize before you put it into the box.
Quote:
FrankHorrigan said: You just need a SAB to work with petris and agar 
Agreed, I just hate pouring in a SAB. Would rather do the no pour myself. Probably gets easier with practice, but the only time I find I need pour agar is for antibiotics (which I recently started with and they are worth the hassel of pour agar), and I actually prefer how much easier the mini rounds are for me to handle compared to a petri in the SAB. Maybe I just built up some good technique with the minis, but I feel so clumsy with real petris
Edited by Pastywhyte (01/16/14 09:14 PM)
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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cool man. i'll be sure and post up, especially if it fails. it'll be a funny sight... a dozen or so smushed cups of seaweed goo.
also, i tried operating the minicups one handed. i guess my hands are too small to make it work, i can't open them one handed. how do you do it? cause i'm sure it's real easy to explain in text format.
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the j stands for jesus.2020 new years grow along
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Can I do no pour like this in 4 oz (1/4 pint) glass wide mouths? If so I assume you'd just wanna make an injection port and leave the lid loose with the seal down so you can seal it afterwards or Does agar need GE at that stage?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Quote:
J. Jack Flash said: also, i tried operating the minicups one handed. i guess my hands are too small to make it work, i can't open them one handed. how do you do it? cause i'm sure it's real easy to explain in text format.
I just hold the lid (already cracked so its loose) with my thumb and forefinger, then cup the rest of it with my other fingers and palm.
I guess I should mention that I'm 6'3" so maybe my hands are just that much bigger.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Quote:
Happy Littletree said: Can I do no pour like this in 4 oz (1/4 pint) glass wide mouths? If so I assume you'd just wanna make an injection port and leave the lid loose with the seal down so you can seal it afterwards or Does agar need GE at that stage?
You sure can, I have done so myself and they work quite well. Just they are usually pricey, I dislike fumbling with rings and lids, and the sides are hard to see through. You don't need an injection port. Just a hole for the vacuum and a filter of some kind. You need to lift the lid to make transfers anyway. They were what I used (along with half pints, fancy jelly jars, screwtop ziplocks, and poptop ziplocks) before I found the glad minis, which at first I never tried cause I thought they were too small
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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So I do have to have a hole and filter not just the jar lid left loose? The reason I ask again is it seems like petris wouldnt have GE and they do fine and the lid cracked should more than take care of the pressure issue. These have a nice clear side to see through unlike most, luckily. I had intended on using these for brf cakes as I couldn't find the ones I need but after I found out about the pp5 I got some small rubbermaids with screw lids for that. Thanks for your help and advice!
Edited by Happy Littletree (01/16/14 09:39 PM)
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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Hate to mutilate my lids if I don't have to
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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petri's do have ge
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Pasty Agar Tek [Re: cronicr]
#19430918 - 01/16/14 10:40 PM (10 years, 13 days ago) |
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Even if the lid is cracked it will still probably suck shut when the vacuum builds. Its not mutilation, its modification. We do lots of that in this hobby.
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 1 year, 2 months
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gotcha.. just wanted to be certain. I been reading a ton and have been reading old information for years but I still have lots of questions If I wanted to make a couple sealed with a port to inject spores into then put in sterile water later for a culture syringe is that feasible? Should I not bother and just do a liquid culture? I'm looking to do some isolation but right now I just wanna get it going. I also plan to do either a syringe to WBS or wait on the agar isolation for a WBS to already composted cow poo. I have gypsum but kinda confused when and when not to use it. Any suggestions welcomed! Big thanks to all the cool people on here that stop and take the time to mentor others!!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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I wouldn't inoculate a liquid culture with anything but an agar wedge. If you want you can inject sterile water into the plate then give it a little swirl then aspirate it back into the syringe, but inoculatiom with a wedge is prettu safe amd you can G2G from there.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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and skip the composted cow poop, you want field aged stuff for proper results, also don't over think gypum, chuck handfulls in here and there. glad to see this thread has sparked some fiire for people, good shit pasty
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: cronicr]
#19431984 - 01/17/14 05:59 AM (10 years, 12 days ago) |
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Quote:
cronicr said: and skip the composted cow poop, you want field aged stuff for proper results, also don't over think gypum, chuck handfulls in here and there. glad to see this thread has sparked some fiire for people, good shit pasty
QFT on all fronts!! (except cow poop, don't know much about it but i'd trust cron on it)
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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