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InvisibleViolet
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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214426 - 12/01/13 07:48 PM (10 years, 1 month ago)

Nice, Pasty!

Quote:

Pastywhyte said:
Quote:

Yuri.Pono said:
how many times have you used this tek with them same type of glad jars. just wondering how many uses til the warp & are useless?



I have done as many as 10 runs with the same containers. Sometimes I get a bad batch that don't last as long. I'd say an average of 5 runs would be fair. That works out to 40 plates for $4. Since I started with these I could not say how many times I have done these, but I am doing about 25-30 a month.



Those should last you far more than 5 uses.
Mine are screw-top of course but I am quite sure that many of mine have had more than 50 runs in the cooker.  All of them have certainly had more than 10 runs.
Only visible wear is scratching from using scrubbies a couple times to clean them out.

Also I never get a "bad batch" of the screw-top ones, at least not with zip-locs or the most similar generics (the purple/blue lid kind they re-label for different marts)

I'll add, screw-top containers don't need lid modifications!


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19343015 - 12/29/13 06:43 PM (10 years, 1 month ago)

Twist-top forever :raver2:


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Re: Pasty Agar Tek [Re: cronicr]
    #19343030 - 12/29/13 06:48 PM (10 years, 1 month ago)

I've never seen Ziplocs for less than $2.25 on sale for 3 pints.
Other brands yeah, but they're still not even worth it


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Re: Pasty Agar Tek [Re: cronicr]
    #19352715 - 12/31/13 08:37 PM (10 years, 29 days ago)

Quote:

cronicr said:


i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops




20-25mL, just like petris...
500mL still makes 20+


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Re: Pasty Agar Tek [Re: cronicr]
    #19352726 - 12/31/13 08:41 PM (10 years, 29 days ago)

Happy new year chron, pasty, everyone!


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19427958 - 01/16/14 01:01 PM (10 years, 13 days ago)

This tek's purpose is to be entry-level, yes?
It does a good job of that in form and instruction, but I feel it can be trimmed even further.

Yours:
Quote:

You will need:
-1 pack of Glad mini rounds
-micro pore tape
-a drill with a 1/4" bit or very hot nail
-paper towels
-Foil
-PC
-Bar of agar agar
-Potato flakes
-Karo or Honey
-Coffee grinder
-And of course a PC, even a shitty ass one will work



Mine:
Quote:

You will need:
-Ziploc twist-top pint containers
-Agar-agar powder
-Grainwater from brown rice/grass seed prep, or malt/karo/potato flakes etc.
-And of course a PC




Maybe I missed it, but why do you use foil for instance?


--------------------
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Re: Pasty Agar Tek [Re: MudaFuka]
    #19428061 - 01/16/14 01:26 PM (10 years, 13 days ago)

Quote:

MudaFuka said:
the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space.



Even "taking up a lot less space" is partially a drawback, because they have so much less grow-out area.
I get to an isolate in very few dish transfers because mine have far more area to run out and begin clearer sectoring.
And once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8.
Additionally, I see that everyone's agar is Very deep in these mini-rounds due to the curvature towards the bottom.  They'd have even less area to grow out if agar were used conservatively.  However mine have as little as a normal petri dish with the wide area.

I can only see taking up less space as being assuredly a good thing in the case of refrigerator storage as Pasty mentioned.
Yet I have had more screw-top dished cultures in the fridge than I even want to, and it hasn't become an issue for me except when I have 20 cultures awaiting the results of elimination testing, in which case fridge storage is still optional...


Quote:

blindingleaf said:
pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers.



I'm sure this is the (or at least a) correct answer.  It's just a bit of a shame though, needing foil because of needing micropore tape because of needing to modify because of not choosing ready-to-use containers...


--------------------
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Re: Pasty Agar Tek [Re: MudaFuka]
    #19428156 - 01/16/14 01:43 PM (10 years, 13 days ago)

Quote:

MudaFuka said:
I don't understand why you seem to care so much about what other people use.



Cut that out.  I'm just talking about materials and process.  I don't "care so much about what other people use" anymore than anyone else does discussing what they do and why.

Quote:

MudaFuka said:
not every one has a large PC.



I have an AA910. Very small.  I doubt that more than a handful of people buy AAs smaller than 916.

Quote:

MudaFuka said:
even with the lid off and the dishes stacked inside one another I can fit fewer screw tops in my PC than mini rounds.



I don't doubt this, I simply don't think it totally makes up for the other factors.

Such as, "once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8."
Do you fit Over Double the glad-rounds in the pc?

Personally I don't even use all the dishes I make in my cooker.  Some of mine sit in sterile airflow for weeks before I find something to drop in them.

Quote:

MudaFuka said:
Iv found I use much more agar filling screw tops than mini rounds.



You use less than ~22mL in the mini rounds?

Even if so, the lesser amount surely doesn't make up for the proportion of lesser area.


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Re: Pasty Agar Tek [Re: PussyFart]
    #19712684 - 03/18/14 11:04 AM (9 years, 10 months ago)

Quote:

PussyFart said:
Petris don't really require a lot of GE, but nothing in this hobby is ever completely sealed.



I completely seal my ziploc containers, until fully colonized despite how they're being used.
The agar dish ones aren't ever opened unless inoculating from them.  They can get tossed around in the fridge for months.
No parafilm or plastic wrap involved...


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
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Re: Pastywhyte's Easy Agar Tek [Re: Imperfect Iam]
    #20472154 - 08/24/14 09:22 PM (9 years, 5 months ago)

Quote:

MudaFuka said:
I saved some WBS soak water



I wouldn't be certain of ideal nutrition in grain water from anything other than grass seed or brown rice.
All the other grains leave the water too pale and, I believe, low on nutrition unless reduced a lot.

If you intend to use grainwater, start with grass seed or brown rice.  IME those are by far the best grains anyway, for a myriad of reasons.


Quote:

Imperfect Iam said:
Really, I don't recall having any problems, except the consistency was to watery, so Violets recommended amount of grain water must be a little high, or the amount of agar low, unless I fucked it up somehow, but it has came out thin consistency everytime, I think I have only done it twice thought.



I don't see how that could happen unless you're not doing something right.  It doesn't matter what nutrient is suspended in the liquid - the proper proportion of agar should gel it up just right, regardless.

Doesn't matter what the nutrient is:
9-10g agar-agar powder per 500mL solution, or .5g agar-agar powder per 50mL solution (NOT an agar/malt mix etc.)


Imperfect Iam:  Are you doing no-pour agar containers?  Talking about "my" recommended "amount of grain water" suggests you are.
If so, maybe *just maybe* you're using agar that has to be boiled before you load it for sterilization.  Some agar types won't gel unless they're boiled first.  Dunno, just a thought.


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #20472447 - 08/24/14 10:19 PM (9 years, 5 months ago)

Quote:

Pastywhyte said:
Quote:

Violet said:
I wouldn't be certain of ideal nutrition in grain water from anything other than grass seed or brown rice.
All the other grains leave the water too pale and, I believe, low on nutrition unless reduced a lot.




I don't agree. I have used wheat, rye, millet and oat grain soak water and they all performed equally well to the RGS agar soak water I tried as well.



You don't agree with my uncertainty?  hehehe


This much is a fact, at least:
Grass seed and brown rice leave MUCH more of the nutritious starch in the water (which is settled to pour off "clear" grainwater for agar use) and the water they leave behind is so much thicker with nutrition that it can be diluted 2x, even 3x, before it is as dense as grainwater from other grains.

So I wouldn't be at all surprised if grainwater from ANY grain works, because they all leave behind the same goodies the grains have that mycelium loves.
But when I've had containers full of gs/br grainwater next to water from birdseed, the birdseed water has never been worth keeping.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pastywhyte's Easy Agar Tek [Re: kmetric]
    #20476406 - 08/25/14 07:35 PM (9 years, 5 months ago)

I'm curious as to why people are wrapping these in foil at all...

For years I've used nothing but unmodified containers — no foil, no paper towels, no filters... just slightly loosened lids.
Whether for grain or agar, I get flawless results.
It's true I usually remove things from the PC in sterile laminar airflow, but twist-tightening the lid as you quickly transfer them to the still-air box should do just about as well.  I know several people that use these unmodified in still-air boxes and they have no problems.


Quote:

kmetric said:
I see..tiger-dropping 1/2 of a round wouldn't be effective? From what I've seen/read from RR, reading here and TMC, I gathered that you could just cut a small section from the colonized agar to inoculate a master jar, just the more the better.

In any case I'll use whole rounds like you guys suggest and maybe play around with the sectioning later on.




Why bother?  What advantage is there in sectioning that makes it worth the while and materials?
For plastic dishes you throw away after a single use, I definitely see the huge boon of sectored dishes.
But when you can re-use these pp5 containers over and over again for free, I hardly see the point in the effort.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #20477086 - 08/25/14 09:49 PM (9 years, 5 months ago)

It's not at all about "my methods" tho, friend.
You don't think you could just sterilize them with the lid not snapped on? Either partially or fully off.  I have done it before with square food storage containers.
For sure I've taken en masse prints several times that way, I even snagged a photo once of printing foil in a square container I sterilized with the lid just sitting loosely on the edge of the container:


Once I learned that foil isn't necessary to cover SFDs back when I still used jars, I stopped using foil in the cooker for everything, except wrapping my tools to keep them sterile until use.
These should be no different.


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #20478702 - 08/26/14 10:03 AM (9 years, 5 months ago)

Now it seems worth investigating why some people experience thrown water content when not using foil.

Because in addition to myself I know lots of growers, including most (if not all) the TCs, that don't bother to cover their filters with foil and obviously have no problems...
and I'm one of a number of growers that use "open" containers, without a filter or anything, that still doesn't have problems with the water content.  My containers are effectively entirely open to the cooker's steam but I have never had an issue.


For they who have water problems when not covering, what could be different about their cooking process that gives them a different experience?

There definitely has to be SOMETHING. Such a different experience has to have a different cause.


--------------------
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Re: Pasty Agar Tek [Re: jerseyian]
    #20550840 - 09/10/14 10:10 PM (9 years, 4 months ago)

They will not melt if you make sure there's water in the cooker!
(Recycling code 5 plastics)

If you run out of water... prepare for melted plastic!


--------------------
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Re: Pasty Agar Tek [Re: Sanguin3]
    #20566624 - 09/14/14 02:11 PM (9 years, 4 months ago)

Quote:

Pastywhyte said:
Sure, Violet uses them all the time. I have used them but I find they take up too much space in both my PC and fridge. But go ahead and use em if you prefer or its all you can find.



They do take up more fridge space (I only need a few in the fridge tho) and fewer do fit in the PC.
But I can use them for literally Every facet of my grow, from culturing to the full grow and more.

And, they can be stacked for agar dish use, meaning you can fit double in the cooker.
http://www.shroomery.org/forums/showflat.php/Number/19035259#19035259
Quote:

I often sterilize them loose-lid as if they were full (25-30min 15PSI for 8, the space of 7 quart jars in a 16qt cooker) but I've found another trick works too particularly when using sterile laminar airflow:

Here 4 containers are stacked loosely into each other, each with agar already loaded, and 4 stacked lids on top (bottom lid screwed loosely, not air-tight on the top container).  This way 4 inter-stacked containers take the space of 2 lids-on containers (30-35min 15PSI for 16, the space of 7 quart jars in a 16qt cooker).
I use this same trick for sterilizing containers & foil for sterile prints!

I've done it several times now, no implosions.  Have had only one warp which I attribute to it being set it snugly thus nearly air-tight.

I suggest observing how snugly they fit into each other before trying this. I find that different containers can be nearly air-tight when fit into each other this way. Try just a couple in a run. If it doesn't work out, or if you don't want to find out the hard way, just sterilize them as normal instead of stacked!

After sterilized, simply remove the containers from the cooker into the sterile workspace, and in a single fluid action with both hands, place one hand's thumb and finger on the 2nd container from the bottom:

... lift, leaving the bottom container as you set the others next to it:

... while taking your other hand to the top lid:

... and placing that top lid on the lidless container. Repeat until placing down the top container which is already lidded.
It's easy and quick, much moreso than lifting stacks of single-use plastic petri plates and pouring warm/hot agar into them in turn.






--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pasty Agar Tek [Re: midnightmaraude] * 1
    #20574383 - 09/16/14 08:44 AM (9 years, 4 months ago)

Quote:

midnightmaraude said:
I read somewhere that you shouldn't use them til 2 days has passed??



This is mainly to see if any poured plates were contaminated during the pouring process.
Bacteria would show up in that time, and mold likely would in two or three days as well.
It just stops you from putting an important inoculation to a dish that is unknowingly already contaminated;
a concern eliminated by using sealed and no-poured agar dishes.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20637904 - 09/29/14 09:47 PM (9 years, 3 months ago)

Quote:

MudaFuka said:
In the past when I have had to much condensation on my plates I just dumped the excess water onto the floor of my SAB right before inoculating them.



^^^^^^^^^^


--------------------
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Re: Pasty Agar Tek [Re: Edmunter]
    #20707275 - 10/15/14 05:03 PM (9 years, 3 months ago)

Quote:

Imperfect Iam said:
I have never had the problem of to stiff agar, but have had it not stiff enough, using the measurements from the v-tek, grain water agar, it made it really suck to try and transfer



Think I said it elsewhere too, but your agar gelling problems are not due to grain water agar.
The agar-agar measurements I gave are no different than for any other agar mixture.  2% agar weight to liquid volume.

Quote:

Edmunter said:
So what does agar do when its too stiff or not stiff enough?



"Too" stiff, it just makes it super firm.  It can kindof seem dry by relation, but mycelium can grow on it too.  If it is WAY too hard it may support only thin growth.  But mostly it's just a waste of materials.
If it's not hard enough, jell-o soft, it makes it a pain to transfer if not impossible.  But there are a few little-known grow techniques that involve using very soft agar at just like 1/4th of the agar (.5% weight to liquid volume) and mycelium grows on it wonderfully.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

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The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20712611 - 10/16/14 07:03 PM (9 years, 3 months ago)

Quote:

Pastywhyte said:
Also do not toss these, if they have sporulated mold you can PC for 30 min and then empty them. I have some that I have probably used 25-30 times now.



I just rinse them then wash them out with a soapy sponge.
My techniques are substantially more contam-proof than ones most growers on here are using, but even when that wasn't the case (and if it weren't now)  I wouldn't be nearly so concerned with sterilizing contaminated containers.  Ideally the sink you're cleaning your dishes in is in a different place than you're growing, too, so even if concerns were valid to a noteworthy degree I'd consider it moot.

Just clean them as you would any other :2cents:


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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