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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: TheApprentice] * 6
    #19209950 - 11/30/13 07:51 PM (10 years, 1 month ago)

awesome TEK!  I got some personal PM tutoring from pasty about this last week. I have transfers growing on them right now, will post pics as soon as the growth is big enough to see (just a tiny wedge now).  I dunno why, I hate pouring agar, I love doing transfers and stuff, but I dread the pour like I dread going to work.  With this its all ready to go after it cools in the PC!  I can't help but think this will encourage a lot more beginners to start agar.

YO THAT SHIT SO PASTY YO!  (sorry I work overnight at a convenience store in the ghetto, the lingo rubs off occasionally....)

:rofl:


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Re: Pasty Agar Tek [Re: cronicr] * 1
    #19217970 - 12/02/13 04:10 PM (10 years, 1 month ago)

is it me or does this growth look....pasty  :smile:





thx again for the tek man...its obviously working


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19264218 - 12/12/13 03:50 AM (10 years, 1 month ago)

question:
if these plates are "poured" in larger quantities (say 3 TBS instead of 1 TBS per plate) would they make for longer term storage in the fridge for cultures because of the increased depth (wrapped in plastic/bagged up of course)?  I do not have $ for slants right now, or will I in the next 2 months.  I have some untested cube isolates, as well as maitaki and skiitaki clones taken from grocery store that grew out clean, all of them r on these pasty plates, and time restrictions r forcing me to delay the starts of newer grow projects.  how long do u think these could last in fridge with a larger amount of agar...and would it be beneficial to tilt them a bit, like with a slant, while cooling if this was to be done?  I have had this happen inadvertently while they cooled in the pc, and those plates are a bitch to transfer from because the agar is so deep in the middle of the culture, but it got me thinking about the relationship to the agars depth and its shelf life.

Im going to have to do it either way but I wanted to throw it out there for opinions.

also might as well ask...what is the longest any of you has kept a petri/easy plate/no pour plate WITH growth in the fridge and it still remain viable?  I personally have used colonized grain jars after 5 months with success, but have never used (or tried) agar from fridge that was longer than a month.
(Pasty, I can move this to a new thread if u want, its not meant to thread jack, but I put it here because ur plates can be poured to a larger depth than petris, so i thought it was related enough to make post here, and if it works, u can add the info @ the end of ur tek? :shrug: )


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19345102 - 12/30/13 05:49 AM (10 years, 30 days ago)

the shop n bag 2 blocks from me in the city of brotherly love has the them both, twist and pop tops.  i think they (shop n bag) r franchised though, so it may be up to the owner of the store what to carry.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: blindingleaf]
    #19351289 - 12/31/13 02:06 PM (10 years, 29 days ago)

pasty i blame u for having too many plates to work with.



gonna have to dump some.  no room in fridge and all jars are in use, so i can't noc up more grain yet.  many of these have grown to the edge, which i haven't had a problem with yet taking wedges up against the walls, but with regular petri i almost always get a bacteria contam if i take a wedge from the edge.  haha sounds like a good thread name "Wedge from the Edge"
what is ur opinion on taking a wedge from the edge of these PP5's vs a regular petri?  am i just lucky or are the higher walls offering a better barrier?


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19351661 - 12/31/13 03:52 PM (10 years, 29 days ago)

yea i seem to have better luck with agar work than g2g.  none of my wedge to grain jars contam ever, but one in 15 jars of g2g will contam.  i am using postal tyvek on these, one layer over the lid, secured down by rim, and have done this with success before.  i think i need a taller SAB because many times, the last of the master jars grain will be difficult to shake in the last g2 jar without jostling around a lot in the say, and letting the two jar rims touch. can't wait to get electric drill to make the plastic lids.  i have sfd's but only six, so when they are in use, i go tyvek until i can turn those six into 36


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A few thoughts on cultivation
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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19355743 - 01/01/14 06:15 PM (10 years, 28 days ago)

chronin
how firm r u letting it get? id imagine u can get it all the way firm and put it in because its just going to liquify again in PC, but r u letting it firm up to just to the point where that middle agar blob happens?


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19355826 - 01/01/14 06:38 PM (10 years, 28 days ago)

cool sounds good.
can u explain to me the theory of not letting pc totally cool, i.e. pc drops to 0, but is still hot, so when u take weighted gauge off, steam comes out for maybe 10 seconds.  there was/is talk of how that may be introducing con tams via outside air being sucked in while steam is being released thru top vent.  is this true?

edit: i have also read that if u don't let it cool completely, like in above example or say when PC is at 1psi, and u take weight off, air will also be sucked out of jar thru filter as the steam/air in PC RUSHES to the vent to escape. is this true also, and is this a concern for contam or for something else?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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Edited by blindingleaf (01/01/14 06:40 PM)


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19355935 - 01/01/14 07:13 PM (10 years, 28 days ago)

ok, i was reading GGMM and he mentions two kinds of PC/autoclave one of which will create a vacuum and the other which will not.  it suggests that if u have the one that does not create the vacuum to take the jars out right when pc drops to 0psi.  i have an AA 914 i believe (the one that fits 7 quarts) with that weight that comes off, is circular and have 5, 10, 15 holes.is the one i have creating  a vacuum?  i'd assume no, at least it doesn't seem like it when i open it, even with weight on top.

GGMM page 132, chapter 15 on generating grain spawn.

I'm asking because i let my jars cool in pc overnight.  whether i use postal tyvek or SFD, i can't help but think the filter is moist in that humid PC, and taking it out say an hour after it has dropped to 0 (so PC still hot and when weight is taken off steam vents for a few seconds) and letting jars cool on shelf would keep the filter dry.  maybe this is all in my head, the concern comes more so when using postal tyvek filters when i run out of SFD lids


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19356480 - 01/01/14 10:42 PM (10 years, 28 days ago)

so whippy and chron...
are u guys waiting till PC cool to the touch to open or just till 0psi?  i remember several grain tek preps that stated to pull jar out a little after 0PSI to shake it and distribute moisture and no mention of possible danger, but then there are several more posts indicating the possible contam issue with this practice.... just made me wonder i guess which i should b doing particularly with the postal tyvek cause i trust my sfd no matter when they're taken out, have had 0 issue with them


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: Forrester]
    #19371541 - 01/05/14 06:43 AM (10 years, 24 days ago)

forrester, do u mod the lid still like in the OP when u pc if u don't use foil?  i remember pasty and I talking about (thru a PM, sry pasty don't hate me :smile: ) lessening the foil, and IIRC we both weren't sure if it would work, for both wetness of micropore and longevity of sterility.  i don't need to worry about foil now (got a HUGE roll for x mas from my sister oddly enough hahah) but i'd like to know regardless.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: Delection]
    #19377776 - 01/06/14 03:30 PM (10 years, 23 days ago)

we have those to where i work, but they are PP2...are those PP5?
if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19378120 - 01/06/14 05:01 PM (10 years, 23 days ago)

haha yea i guess ur right.  but i thought with the pasty tek we have to raise them a bit above the water line?  i always do anyways...i guess to prevent over boiling?

edit:  just re read tek, I'm an idiot :shrug:  no mention of raising above water level.  guess I'm paranoid too haha


Edited by blindingleaf (01/06/14 05:06 PM)


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: MudaFuka]
    #19422697 - 01/15/14 12:52 PM (10 years, 14 days ago)

if u end up finding ur mind, put 5g of it in pasty agar recipe and u will see crazy rhino growth!


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: DeadPhan]
    #19423061 - 01/15/14 02:11 PM (10 years, 14 days ago)

haha no, mudafucker said he was losing his mind, so i said if u find it, put 5 g of ur mind in the recipe.  it was a joke...guess not a funny one  :shrug:


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19423549 - 01/15/14 03:51 PM (10 years, 14 days ago)

cool, thought it went over ppls heads haha.


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Re: Pasty Agar Tek [Re: Generic]
    #19427948 - 01/16/14 12:58 PM (10 years, 13 days ago)









this is not what it looks like (or maybe it is...)

so this holiday season was crazy for me, let 28 plates go too long.  had to toss them and start a new.  this is really a testament to not only my laziness (I'm on top of stuff now), and the lack of space in my fridge, but also to Pasty's TEK.  these ALL lasted OVER A MONTH AND A HALF with NO refrigeration, survived multiple transfers, dust from smoke, cats, and a dog, and no extra seal, nada.  i didn't even keep the unused ones in fridge till use, they all stayed room temp with no extra filter, in a cardboard box.  just wanna say thx again pasty, ur tek is awesome, and i wanted to post these here so ppl kno u can almost abuse these things if u make them to a t like the tek, and have little contam (if any, maybe i was lucky...?).  In the trash bag pic, thats not a brown contam, thats shiitaki left too long, and browning up a bit in case anyone wondered.


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Re: Pasty Agar Tek [Re: Violet] * 1
    #19427968 - 01/16/14 01:04 PM (10 years, 13 days ago)

pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers.  i had asked him this (in PM) kinda about shaving off the amount of foil.  i tried with no foil, too much water, but this is what i came up with



instead of wrapping the entire container.  works for me, just gotta really press the foil under the lip hard.  i just wanted to save foil  :shrug:


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19430095 - 01/16/14 08:18 PM (10 years, 13 days ago)

yea u can def drop a whole snap lids worth of agar into a grain jar (cut it up first though).  i tried the pasty tek with no tin foil, water got thru the micropore.  when i tried it with foil covering the cracked lid and no hole, water still got in.  that hole is not for GE in this TEK, its meant for gas to escape when the container is actually cooking (correct me if I'm wrong though pasty).  if u have luck with no lid mod, let me know.  if u wrap the entire container with foil (like the OP tek says) u can probably get away with a cracked, no hole lid.  but thats a lot of foil.
post results if it works!


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Re: Pasty Agar Tek [Re: cronicr]
    #19431984 - 01/17/14 05:59 AM (10 years, 12 days ago)

Quote:

cronicr said:
and skip the composted cow poop, you want field aged stuff for proper results, also don't over think gypum, chuck handfulls in here and there. glad to see this thread has sparked some fiire for people, good shit pasty




QFT on all fronts!!  (except cow poop, don't know much about it :shrug: but i'd trust cron on it)


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