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InvisibleSpitballJedi
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Re: Does using a SAB without a lid defeat the point? [Re: PussyFart]
    #19206106 - 11/29/13 07:26 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
You might as well just work in open air if that was the case....it would be the same thing.

Try flipping it and putting it on a wet towel like I do......


   



:whathesaid:

That's what I do

My SAB


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InvisibleMUSH HEAD420
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Re: Does using a SAB without a lid defeat the point? [Re: SpitballJedi]
    #19206168 - 11/29/13 07:45 PM (10 years, 2 months ago)

I like the amount of jars NAH can fit in his, that means he doesn't need to fuck around with only a couple jars at a time. If height is an issue and you can't poor from one jar to the other, just use a spoon. As long as the spoon never touches anything other than some disinfectant and spawn you should be good.


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InvisiblePastywhyteMDiscord
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19206179 - 11/29/13 07:47 PM (10 years, 2 months ago)

Spoon?


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InvisibleSpitballJedi
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19206188 - 11/29/13 07:49 PM (10 years, 2 months ago)

Using a spoon is risky, especially if you put disinfectant on it.

If height is an issue, get a taller SAB like I did


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Edited by SpitballJedi (11/29/13 07:49 PM)


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InvisibleMUSH HEAD420
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Re: Does using a SAB without a lid defeat the point? [Re: SpitballJedi]
    #19206209 - 11/29/13 07:54 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Using a spoon is risky, especially if you put disinfectant on it.

If height is an issue, get a taller SAB like I did




I seen people use a spoon without a problem, I guess you should know better than most though.

I seen RR totally soak jars and his gloves in disinfectant.


Edited by MUSH HEAD420 (11/29/13 07:56 PM)


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Offlinekrypto2000
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19206328 - 11/29/13 08:23 PM (10 years, 2 months ago)

I use to have nearly 100% success with using a spoon in the open air to do g2g transfers, but not since I've moved. Actually I haven't even tried it, but less risky things have proven unsuccessful so I didn't bother.


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Offlinemthawkins
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Re: Does using a SAB without a lid defeat the point? [Re: krypto2000]
    #19206626 - 11/29/13 09:53 PM (10 years, 2 months ago)

Would it be sufficient to completely scrub down the bathroom clean and work in open air next to the sink? Move very slowly without breathing? lol


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OfflinePussyFart
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Re: Does using a SAB without a lid defeat the point? [Re: mthawkins]
    #19206630 - 11/29/13 09:54 PM (10 years, 2 months ago)

Quote:

mthawkins said:
Would it be sufficient to completely scrub down the bathroom clean and work in open air next to the sink?



Injections maybe.....anything else like g2g transfers or agar transfers, no.


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Offlinekrypto2000
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Re: Does using a SAB without a lid defeat the point? [Re: mthawkins]
    #19207634 - 11/30/13 07:53 AM (10 years, 2 months ago)

Quote:

mthawkins said:
Would it be sufficient to completely scrub down the bathroom clean and work in open air next to the sink? Move very slowly without breathing? lol




I've never thought of the bathroom as a clean place, I mean you shit in there. In general depending how clean your ambient air is you might be able to get away with it by whiping very thoroughly with iso and spraying lysol everywhere, but you're taking a chance. It might work, it might not, you can't fully control what's in the air like with a SAB or flowhood so it's unreliable at best.


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InvisiblebodhisattaMDiscordReddit
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19207772 - 11/30/13 08:45 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
I like the amount of jars NAH can fit in his, that means he doesn't need to fuck around with only a couple jars at a time. If height is an issue and you can't poor from one jar to the other, just use a spoon. As long as the spoon never touches anything other than some disinfectant and spawn you should be good.




Quote:

MUSH HEAD420 said:
Quote:

SpitballJedi said:
Using a spoon is risky, especially if you put disinfectant on it.

If height is an issue, get a taller SAB like I did




I seen people use a spoon without a problem, I guess you should know better than most though.

I seen RR totally soak jars and his gloves in disinfectant.





You can PC your spoon and use it, but even then it's risky to use it for so many transfers. any disinfectant is only sanitary not sterile so don't bother using sanitizers on anything that will touch your grains. We use disinfectants/sanitizers on our gloves and the outside of the jars because that's the best we can do since we obviously cant sterilize our hands or jar lids, then again our hands and lids never touch the grains.


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InvisibleSpitballJedi
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19208026 - 11/30/13 10:11 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
Quote:

SpitballJedi said:
Using a spoon is risky, especially if you put disinfectant on it.

If height is an issue, get a taller SAB like I did




I seen people use a spoon without a problem, I guess you should know better than most though.

I seen RR totally soak jars and his gloves in disinfectant.




Do you have a link to RR doing this? I've seen him use alcohol on the outside of jars and his gloves, but he doesn't stick his hands in the sterilized jars.


I know better than some, but not as much as others. The spoon can be used successfully, but it's risky. There are better methods to increase chances of success.


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InvisibleMUSH HEAD420
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Re: Does using a SAB without a lid defeat the point? [Re: SpitballJedi]
    #19208032 - 11/30/13 10:14 AM (10 years, 2 months ago)

I agree completely.


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InvisiblePastywhyteMDiscord
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Re: Does using a SAB without a lid defeat the point? [Re: MUSH HEAD420]
    #19208218 - 11/30/13 11:21 AM (10 years, 2 months ago)

In mycology. . . There is no spoon

:blueorred:


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InvisibleMUSH HEAD420
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Re: Does using a SAB without a lid defeat the point? [Re: Pastywhyte]
    #19208390 - 11/30/13 12:11 PM (10 years, 2 months ago)



+



NO? lol I'm a tard.


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Offlinenn-IlliniSpiralDMT
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Re: Does using a SAB without a lid defeat the point? [Re: Pastywhyte]
    #19208404 - 11/30/13 12:15 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
In mycology. . . There is no spoon

:blueorred:




:wtfsonic::lmafo:


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OfflineBigGreenMat
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Re: Does using a SAB without a lid defeat the point? [Re: nn-IlliniSpiralDMT]
    #19208840 - 11/30/13 02:24 PM (10 years, 2 months ago)

Actually it is really simple to sterilize a spoon.  Just dip it in alcohol and then flame to burn off the alcohol.  Sterilized spoon.  I do it all the time when doing bacterial culture plating with a loop.  From what I have seen the major issue is a lot of guys using alcohol soaked EVERYTHING in a SAB which can lead to flammable alcohol vapor.  I have been meaning to ask some of the oldboys about possible mods or changes so you could have an alcohol bath along with a flame in the box so you can resterilize tools inside the box.  Also you can flame the things you touch for a bit of added sterility (this is how we do it in the lab when doing cell culture).


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InvisiblePastywhyteMDiscord
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Re: Does using a SAB without a lid defeat the point? [Re: BigGreenMat]
    #19208864 - 11/30/13 02:33 PM (10 years, 2 months ago)

Quote:

BigGreenMat said:
I have been meaning to ask some of the oldboys about possible mods or changes so you could have an alcohol bath along with a flame in the box so you can resterilize tools inside the box.




Sounds like :onfire:


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InvisiblePestile

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Re: Does using a SAB without a lid defeat the point? [Re: BigGreenMat]
    #19208963 - 11/30/13 03:05 PM (10 years, 2 months ago)

I do not believe you will reach an adequate sterilizing temperature by dipping the said tool in alcohol and igniting it. :smile:


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OfflinePussyFart
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Re: Does using a SAB without a lid defeat the point? [Re: BigGreenMat]
    #19209265 - 11/30/13 04:50 PM (10 years, 2 months ago)

Quote:

BigGreenMat said:
I have been meaning to ask some of the oldboys about possible mods or changes so you could have an alcohol bath along with a flame in the box so you can resterilize tools inside the box.



The heat currents would mess up the "still air" environment and create drafts.


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OfflineBigGreenMat
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Re: Does using a SAB without a lid defeat the point? [Re: PussyFart]
    #19209431 - 11/30/13 05:44 PM (10 years, 2 months ago)

Yeah, I am aware of the effects flames can have in a flow hood situation (although they also have benefits).  Whatever, changes in airflow or turbulence they cause still might be worth the merit of use for sterilization of tools, especially an activated flame.

This is actually a very good primer on sterile technique.  http://georgelab.eng.uci.edu/resources/Aseptic%20Technique.pdf


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