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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Home made MEA expiriment, my observations
    #19207043 - 11/30/13 12:25 AM (10 years, 2 months ago)

I made some agar plates and it wasn't quite what I expected.  If anyone has some light to shed on my methods please do.

I've used prepared petri plates before from a non shroomery vendor and they were ok, but what I didn't like was how loose the lids were and having to tape them shut.  So to solve that problem I made some MEA according to the recipe on shroomery.  This one to be exact:

Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water

I used some 1/4 pint jars instead of petri dishes.  I like these way more because the lid goes on and off easily and the seal should be tight.  Sealing and unsealing tape is a total pain in a glove box, while wearing gloves.

After pouring the hot liquid, some jars had a head space of 1/2 cm others had closer to 1 cm.  The 1 cm ones seemed to have done a little better, and by that I mean more visible growth of mycelium. The ones with 1/2 cm had growth but it was so whispy as to be almost invisible except if you held it so the light caught it.  Even the ones that did well were not very thick, not anything like the growth I'm used to seeing on my own petri dishes (store bought) or on pictures on the shroomery, or RR's videos.  It seems like I should have used a little more head space in the jar, and maybe some additional nutrients?

For transfer material, I used an agar plate that was fully colonized from a pin that I liked, so I took out small agar wedges and dropped them into my jars.  When I opened them up about a week later most of the wedges looked like they did when I placed them in.  The myc didn't grow on the sides of the wedge nearly as strongly as it had grown when in the original plate.  It was thin and weak looking.  So I think maybe a lack of oxygen is to blame.


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