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Offlinedr.alkaline
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Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
Spore print woes
    #19205865 - 11/29/13 06:14 PM (10 years, 2 months ago)

I know spore from prints can take several weeks to germinate on agar, but I innoc'd some agar with spores one month ago and no sign of growth. Is it time to give up on these guys? Please look at my procedure and see if I am making an error. :sad:

I have 6 agar plates that I inoculated from 6 different prints from 6 different caps. I used spores fresh from prints generated a day beforehand. I flame sterilized my loop, quenched it in agar, and rubbed the loop on the print a bit before transferring to the agar with circular rubbing motion. Agar from this stock of plates was used to successfully grow mycelium from fruits.

I take my spore prints by placing the cap on a sterile foil square in a plastic tuperware container (etoh sanitized), covering the cap with fresh laundered tyvek, and leaving the tuperware lid ajar for 48 hours while the cap drops spores. Once 48 hours has passed, I remove the cap, replace the tyvek, and again leave the tuperware jar lid ajar for 24 hours to allow the print to dry. After this drying period is up is when I inoculated my agar with spores. All of this is done in still air box. My sterile technique is very good.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
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Re: Spore print woes [Re: dr.alkaline]
    #19206072 - 11/29/13 07:14 PM (10 years, 2 months ago)

when shit like this happens to me i use syringes to hydrate the spores and the no pour agar tek


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinedr.alkaline
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Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
Re: Spore print woes [Re: cronicr]
    #19206104 - 11/29/13 07:25 PM (10 years, 2 months ago)

Doe this really work much better in your experience? Agar has a very high water content, how are the spores prevented from hydrating on the surface of the agar?

I am kind of a noob and I am not at all discrediting your methods, just trying to better understand how this all works.


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