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Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Welp, I fucked up pretty badly. [Re: lessismore]
    #19199701 - 11/28/13 08:02 AM (10 years, 2 months ago)

I will recommend an alcohol lamp to you, very good for mycology, saves lots of time and makes everything easier
sterilize red hot outside, bring needle inside

I don't even use gloves... maybe one day.. so far good luck with agar,cloning,lc etc.

you can make a magnet stirrer with a PC fan too+harddisk magnet for your LCs, and put a small nail in it
makes everything much easier


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OfflineKalypto
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Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
Re: Welp, I fucked up pretty badly. [Re: Pastywhyte]
    #19199853 - 11/28/13 08:57 AM (10 years, 2 months ago)

Quote:

Pastywhyte said:
OP get a torch. Lighters are slow and bring your alcohol soaked gloves too close to the flame. A torch will get your tools red hot in seconds and you don't risk a stay in the burn ward.



:whathesaid:


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Offlinenn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 4 months
Re: Welp, I fucked up pretty badly. [Re: Kalypto]
    #19200135 - 11/28/13 10:22 AM (10 years, 2 months ago)

Quote:

Kalypto said:
Quote:

nn-IlliniSpiralDMT said:
Quote:

Notahacker420 said:
Quote:

scorpion2458 said:
I covered them with isopropyl alcohol, 91%



Use 70%....it's better than 90% at sanitizing things.




Well isn't 91% more flammable which would mean more heat being transferred to the scalpel? The flame sterilizes not the alcohol





*Facepalm*



Quote:

Notahacker420 said:
Quote:

nn-IlliniSpiralDMT said:
Quote:

Notahacker420 said:
Quote:

scorpion2458 said:
I covered them with isopropyl alcohol, 91%



Use 70%....it's better than 90% at sanitizing things.




Well isn't 91% more flammable which would mean more heat being transferred to the scalpel? The flame sterilizes not the alcohol



We do not flame sterilize our hands...or the latex gloves.......or anything else that is not a needle/scalpel/loop/etc......

70% has a greater water content, which helps penetrate cell walls more efficiently.

This can be googled.




I thought he was talking about flame sterilizing his scalpel...not his hands lol.
Quote:

Kalypto said:
Quote:

nn-IlliniSpiralDMT said:
Quote:

Notahacker420 said:
Quote:

scorpion2458 said:
I covered them with isopropyl alcohol, 91%



Use 70%....it's better than 90% at sanitizing things.




Well isn't 91% more flammable which would mean more heat being transferred to the scalpel? The flame sterilizes not the alcohol





*Facepalm*




Why the facepalm? Simple reading mistake brosk


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OfflineKalypto
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Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
Re: Welp, I fucked up pretty badly. [Re: nn-IlliniSpiralDMT]
    #19200166 - 11/28/13 10:29 AM (10 years, 2 months ago)

We do not use alcohol as a fuel to heat something

We use alcohol to kill things , so 70% is better because it has more water to penetrate cell walls.

Its got nothing to do with transferring heat.Hands or not


Edited by Kalypto (11/28/13 10:30 AM)


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: Welp, I fucked up pretty badly. [Re: Pastywhyte]
    #19200586 - 11/28/13 12:26 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
OP get a torch. Lighters are slow and bring your alcohol soaked gloves too close to the flame. A torch will get your tools red hot in seconds and you don't risk a stay in the burn ward.



:whathesaid:
hotknifing is better with them too


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
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Re: Welp, I fucked up pretty badly. [Re: cronicr]
    #19200611 - 11/28/13 12:30 PM (10 years, 2 months ago)

Quote:

cronicr said:
Quote:

Pastywhyte said:
OP get a torch. Lighters are slow and bring your alcohol soaked gloves too close to the flame. A torch will get your tools red hot in seconds and you don't risk a stay in the burn ward.



:whathesaid:
hotknifing is better with them too



:awesomenod:


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Offlinenn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 4 months
Re: Welp, I fucked up pretty badly. [Re: Kalypto]
    #19204930 - 11/29/13 01:37 PM (10 years, 2 months ago)

Quote:

Kalypto said:
We do not use alcohol as a fuel to heat something

We use alcohol to kill things , so 70% is better because it has more water to penetrate cell walls.

Its got nothing to do with transferring heat.Hands or not




That's exactly why I put alcohol in my lamp, to use as a fuel to create a flame. The water content is irrelevant, the flame sterilizes the scalpel not the alcohol. Yes, 70% is better to spray to clean as it penetrates the cell walls of microorganisms more rapidly than 91% but in a alcohol lamp it is better to use 91% to flame sterilize. 70% is 30% water as compared to 91% being only 9% diluted. Thus it creates a hotter flame, if you had an alcohol lamp you would realize this but I assume you do not:shrug:

The heat kills the unwanted micro-organisms, not the alcohol. If that was the case why wouldn't I just dip my scalpel in 71% alcohol and go to work? Because it wouldn't work, just spraying alcohol doesn't insure that my tools and supplies are 100% sterilized...the flame kills everything regardless of what the fueling agent is. Ethanol, ISO, Butante, Napatha etc etc...


Edited by nn-IlliniSpiralDMT (11/29/13 02:56 PM)


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InvisiblecArcace-x
YeAh!!
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Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
Re: Welp, I fucked up pretty badly. [Re: anne halonium]
    #19205046 - 11/29/13 02:18 PM (10 years, 2 months ago)

Quote:

anne halonium said:

we now use bleach wipes ,
and torches, with no flammables in the work area at all.



:whatshesaid: is a good idea.


--------------------



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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Welp, I fucked up pretty badly. [Re: scorpion2458]
    #19205249 - 11/29/13 03:17 PM (10 years, 2 months ago)

I use denatured alcohol in a wick-less alcohol lamp. I light the lamp before I put 70% IPA on my gloved hands.

The inside of my SAB gets sprayed with a light solution of water and Dr. Bronner's Almond Scented Soap


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