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OfflineBarnabey
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Registered: 06/19/13
Posts: 249
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Newbie strain selection question of the day !
    #19204928 - 11/29/13 01:36 PM (10 years, 2 months ago)

Here is my newbie question :

When germinating spores on agar you try to isolate strains on new petri dishes based on the apparence / growth etc of the mycelium.

When cloning tissue from a mushroom on agar, is there any sort of selection to make out of the mycelium growing ?

I'm assuming it would be only one kind with nothing to isolate since it's just a clone ?

=> What should I do once my petri dish shows enough development ?
=> Is it necessary to transfer a wedge of a tissue culture into a new petri dish before inoculating on grain ?

Thank you very much who ever want to enlight me !


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Newbie strain selection question of the day ! [Re: Barnabey] * 1
    #19205017 - 11/29/13 02:09 PM (10 years, 2 months ago)

Cool!
Make the same kind of selections from clone transfers as direct mycelium transfers.  Healthy, hardy, thick white, fast growth.
A clone from multi-spore is quite likely to have several genetic sets in it. It may begin sectoring right away or after just one or two transfers.
Multi-spore culture agar transfers can take that few or several more to show the same sectoring, and may or may not be fruiting strains which is pretty well confirmed with a clone.

It may not be necessary to transfer a clone if you have no contaminants. You should do it anyway and isolate in a dish or two for best results and to predict culture ability for future use


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