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Offlinesocraticd
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Registered: 08/06/13
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master slants - how much condensation is too much?
    #19204306 - 11/29/13 10:16 AM (10 years, 2 months ago)

After my second shot at making master culture slants, I have found a method that produces LESS condensation, but still have SOME.  on a couple of the slants its just a bit of condensation on the glass, but on a couple others its consolidated to the point that a few droplets worth is poled at the bottom of the slant if its held vertically. 

I had done this with the last round and they all seemed to colonize just fine but I tossed them to start over with a less condensation prone method.


So for all you folks that are using culture slants, how much condensation do you consider OK?

Also, how are you getting condensation a free tubes?    I feel like I have it mostly figured out for Petri's.  But slant tubes are a different story.


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Offlineshopdropper
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Re: master slants - how much condensation is too much? [Re: socraticd]
    #19204496 - 11/29/13 11:20 AM (10 years, 2 months ago)

tilt the slant so the condensation runs away from the colonized agar? :shrug:


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OfflineRogerRabbitM
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Re: master slants - how much condensation is too much? [Re: shopdropper]
    #19206017 - 11/29/13 07:01 PM (10 years, 2 months ago)

There will be zero condensation if done properly.  You'll have some in the first days after pouring, but if you wait a week to inoculate, it will all be absorbed into the agar.

Condensation is caused by a temperature differential between inside and outside the slant.  When you refrigerate, be sure to put the slants in an insulated lunch box or similar and lay in a few of those gel packs to ameliorate the temp swings in your refrigerator.
RR


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