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OfflineQuantus
The Eternal
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Registered: 03/06/12
Posts: 8
Last seen: 10 years, 1 month
First Agar Attempt
    #19201877 - 11/28/13 06:14 PM (10 years, 2 months ago)

I'm about to undergo my first Agar attempt and I was wondering if Im using one strain from a spore syringe and I make 10 cultures from there if i'm going to isolate them, are there a gigantic amount of different genetics ? say I take two cultures from each of ten is there a chance that there will be mycillium that are the same if they came from one syringe ?


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OfflinePsilicon
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Registered: 08/26/12
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Re: First Agar Attempt [Re: Quantus]
    #19202089 - 11/28/13 06:53 PM (10 years, 2 months ago)

In one drop of spores in one syringe are thousands and thousands of spores, none of which are the same due to the wide degree of genetic variability as well as crossing over (where actual parts of chromosomes get traded) and gene conversion, which is a funky process in which one gene gets destroyed and replaced with the another copy.

One drop of spore solution, then, is full of thousands of unique individuals which will never be the same as any other drop.  On average, though, each drop will have about the average number of average individuals.


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OfflineQuantus
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Re: First Agar Attempt [Re: Psilicon]
    #19207645 - 11/30/13 07:58 AM (10 years, 2 months ago)

Alright so I made a 500ml batch using 10g agar agar and 10g of light malt extract. I PC'ed it for 35 min and when it was all cooled and done there was less than 200mL left in the bottle. I used a standard whisky bottle, I put a small hole in the top then put micropore tape over it then tinfoil ? is there something I did wrong did I PC them too long why did I have so much boil over ? Also I successfully poured them into the perti dishes (first time made a few mistakes but overall did pretty well)
then let cool then inoculated them with a spore syringe. Approximately how long until there is signs of growth from either con tams or mycillium ?


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OfflinePsilicon
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Registered: 08/26/12
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Last seen: 3 years, 1 month
Re: First Agar Attempt [Re: Quantus]
    #19208805 - 11/30/13 02:15 PM (10 years, 1 month ago)

I have no idea where it all went.  That's pretty puzzling.  Some loss usually happens, but usually not that much. 

You'll start to see bacterial contamination within 2-4 days at room temperature, and you'll see mycelium anywhere between 4-14 days most likely (but sometimes longer than that).  Whether that mycelium is mold or the intended fungus is another question.


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