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OfflineGloogloos
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Glass vs Plastic petri dishes, cant decide
    #19200675 - 11/28/13 12:41 PM (10 years, 2 months ago)

New to agar culturing here, so im trying to decide on petri dishes, glass or plastic (or even those little ball jars).

Glass - I like the idea of high quality, reusable equipment because A. I don't have to worry about getting new ones all the time and B. I can sterilize them myself.  But is it worth the cost and is it worth the fragility of them?

Plastic - cheap and readily available.  I would rather not have to always ordering them though, and I don't trust packaging to keep them sterile, plastic sleeves tear easily.  Plastic also will let me dick around with different sizes and dividings though, which would be nice.

Jars! - I love jars, and those little 1/4 pint jars seem like they could work.  The feedback seems positive, people like them because they are relatively cheap, durable, available and you can sterilize agar in them, cutting out a step.  However they seem too deep and they seem like they could be difficult to cut wedges out of and they seem like they would be space consuming if you were doing like 50 or more.

Any feedback would be greatly appreciated.  My current idea is to get those jars for this fist attempt, as I feel they are less likely to get contaminated, I can get them locally and I am only doing maybe 10-20 dishes this first time.  I figure I can use these as master cultures and then get disposables for isolating strains and growing cultures to move to grain jars.

Thank you thank you ahead of time.

TLDR: GLASS vs PLASTIC vs 1/4 pint JARS


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InvisibleFrankHorrigan
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Re: Glass vs Plastic petri dishes, cant decide [Re: Gloogloos]
    #19200713 - 11/28/13 12:50 PM (10 years, 2 months ago)

You can re-use glass petris but you still need to sterilize them empty, let them cool, then pour your agar.

You might imagine that you can load the glass petris with your media and then PC them but this is not the case, the agar will just boil over everywhere.

No pour jars are better than glass petris.

But almost every experienced cultivator I've ever talked to uses regular plastic petris, myself included.

Because they are small, easy to work with, and perfect for the task :wink:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (11/28/13 12:58 PM)


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InvisibleSkinty
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Re: Glass vs Plastic petri dishes, cant decide [Re: FrankHorrigan] * 1
    #19200730 - 11/28/13 12:55 PM (10 years, 2 months ago)

I'm using these things:

http://www.amazon.co.uk/GSL%C2%AE-PLASTIC-STORAGE-CONTAINERS-Weekdays/dp/B005VB50W6/ref=pd_sim_sbs_kh_2

(although not form amazon - just showing the product)

PCable...seem pretty perfect for no pour :thumbup:

(but then I am an agar noob - seems to be working out so far :wink:)


--------------------


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Glass vs Plastic petri dishes, cant decide [Re: Skinty]
    #19200827 - 11/28/13 01:17 PM (10 years, 2 months ago)

Gloogloos there's an option you forgot.
Plastic no-pour agar dishes
No regular orders online,  no wasted plastic,  reusable,  no carefully-timed pour procedure,  high sterile success,  no parafilm,  no loose "lids" (petri tops),  wide grow-out area...
... and of course a nearly limitless variety of uses!
The only thing they aren't great for is long-term refrigeration due to their height.


Plastic disposed petris Suck IMHO.
People keep using them not because they're somehow actually the best option but because they're cheap at face value.  However despite their lack of monetary expense, the price of such reckless plastic waste is greater. 5-10¢ for a sterile plastic dish is an artificially low value.

If you use a recycling code 5 plastic screw-top container as such 8-12 times then it's paid for itself and used only a fraction of the plastic, with still more uses to come!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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Invisible36fuckin5
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Registered: 08/11/03
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
    #19200872 - 11/28/13 01:27 PM (10 years, 2 months ago)

I still like regular plastic petris. I use the 60mm ones.

Glass dishes are better for things like crystallization. They're too heavy and slippery to really use for our purposes.

I am completely against the idea of those plastic reusable containers. A wide area to grow out isn't an advantage. It allows much more room for contams. They also use much more agar than needed, and you can't ship them if you wanna trade a culture. You can barely see through them to spot contams. Just not worth it IMO.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisibledusttodust


Registered: 11/26/12
Posts: 491
Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
    #19200937 - 11/28/13 01:45 PM (10 years, 2 months ago)

Truth to tell, i do thing opposite from what Frank said.

I have 6 glass dishes. One cost around 3€. Some argue that those are cheap dishes and will break but i have been using them for some time and they havent broken yet, im satisfied with them :shrug:

I normally pour them before i pc them: After boiling i let them cool to room temperature and i put them in baking bags and than i make knot at the end and i try to squeeze all the air out first of course, than i pc. Its a bit tricky and you have to get some experience first so you dont mess things up, but i find my method very easy and i dont have any problems with it.

I also sometimes pour after i sterilize. Well, its up to how i feel i guess.

I use glass when working with small projects and i can reuse them as many times as i want. If i need to clean up culture id rather use glass than waste many plastic ones.

If i had bigger projects than i would use plastic. Looks more handy and much cheaper.

I also use jars sometimes if i dont have any spare glass dish and if i dont want to open sleeve of plastic ones but its my last resort, i dont really like it.

I think Violets plastic-no-pour tek is also great and cheap and very useful.

There is no rule here, u can use anything as long as its sterile and it works for you. I suggest that if you are beginer, stick to verified methods.

Good luck!


Edited by dusttodust (11/28/13 03:10 PM)


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OfflinePussyFart
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Re: Glass vs Plastic petri dishes, cant decide [Re: dusttodust]
    #19201165 - 11/28/13 03:01 PM (10 years, 2 months ago)

I get 500 plastic dishes for like $70 and I'm good for a year.


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OfflineTheCyndicate
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201219 - 11/28/13 03:13 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
I get 500 plastic dishes for like $70 and I'm good for a year.



Hey hack's you get them locally ? Or online?

Cyn


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OfflinePussyFart
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Re: Glass vs Plastic petri dishes, cant decide [Re: TheCyndicate]
    #19201221 - 11/28/13 03:14 PM (10 years, 2 months ago)

Online...ebay or amazon....


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OfflineTheCyndicate
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201237 - 11/28/13 03:19 PM (10 years, 2 months ago)

:thumbup:

Cyn


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Invisibleanne halonium
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Registered: 05/07/13
Posts: 1,908
Re: Glass vs Plastic petri dishes, cant decide [Re: dusttodust]
    #19201248 - 11/28/13 03:22 PM (10 years, 2 months ago)

Quote:

dusttodust said:
Truth to tell, i do thing opposite from what Frank said.





not opposite in my case, but more advanced.
we grow 2013 style , not 1953 cold war tech style.
we us polycarbonate culture bottles.
all the advantages of glass and plastic combined,
and ,it avoids many of the downsides, of both.



glass or plastic petris,
is not the real choice.
polycarb bottles are.



embrace the future of grow equipment.
order some today!

Quote:

dusttodust said:
I suggest that if you are begginer, stick to verified methods.






i agree.
problem is who verifys?
modern growers, or amish?

if this held true always, everyones first car would be a horse.

modern labs have used culture bottles for some time now.
in spite of modern lab advances, many still dont realize advanced equip is cheap and plentiful.

dont let the community standard, be your outdated standard.
and dont let a fetish for "tradional" become a trap for "outdated"

dont let your grow, stop evolving, cuz other growers fear to evolve.


--------------------
:aliendance:


Edited by anne halonium (11/28/13 03:37 PM)


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201264 - 11/28/13 03:27 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
I get 500 plastic dishes for like $70 and I'm good for a year.



14 cents per dish.
At that price, I can use a container only 7 times and beat that value, while of course still having the container for more countless runs.

70 bucks buys 70 or more containers.  70 containers used only 8 times as dishes makes for 560 dish uses.
And after all those containers' uses, while your 500 disposed dishes are in the garbage, there are still more container uses to come not only as "petris" but myriad other uses as well!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflinePussyFart
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
    #19201287 - 11/28/13 03:33 PM (10 years, 2 months ago)

To each their own.....I like my disposable dishes better, even tho I could get more uses out of other methods, I choose this method.

They take up less space, they are easier to handle IMO....


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InvisibleViolet
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201304 - 11/28/13 03:37 PM (10 years, 2 months ago)

A single petri may take less space than containers when they're not stacked, but is Definitely not easier to handle. Screw-on lids instead of loose lids with parafilm, and no pours.
And 70 containers sure don't take up as much space as 500 petris. So it depends on how you consider it.
It takes such selective considerations to think disposed plastic is better.  But as you said, "to each their own" inefficiency and waste...


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflinePussyFart
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
    #19201417 - 11/28/13 04:14 PM (10 years, 2 months ago)

Quote:

Violet said:
But as you said, "to each their own" inefficiency and waste...



Inefficiency? Seems very efficient to me.... :shrug:


Edited by PussyFart (11/28/13 04:16 PM)


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InvisibleViolet
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201442 - 11/28/13 04:20 PM (10 years, 2 months ago)

Throwing away 1000 pieces of plastic the size of drink coasters sounds efficient to you?
Especially instead of re-using 1/8th of the plastic more than 50 times over?
Maybe you don't mean "efficiency" like I do then.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
    #19201495 - 11/28/13 04:30 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
To each their own.....




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Invisibleanne halonium
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Re: Glass vs Plastic petri dishes, cant decide [Re: FrankHorrigan]
    #19201555 - 11/28/13 04:49 PM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
But almost every experienced cultivator I've ever talked to uses regular plastic petris, myself included.





*you should get out more frank.
modern labs use culture bottles wholesale these days.

i think your refering to just the growers, you know,
who have limited pro lab experience.
hardly a sample of what goes on globally in biotech.

fact is, polycarb culture bottles ,
are a excellent choice. for the informed.



frank always suggests, whatever he does is standard.
sometime it is , sometimes its only his POV.

my standard, is rise to achieve "cutting edge."
yours should be also.
following the pack, is not leading the grow.
its just replicating, and replicating , always winds up outdated.



500 ml polycarb culture bottles, each one has 50 sq cm agar.

no slopping, no breaking, no polyfilm , easily decontaminated, silent, easily stored , and long lasting.
they pour and PC in one shot.

i used both glass and plastic dishes in 80's and 90's
culture bottles contam lower rate IMO by a margin.
unless theres a specific need, i never intend to use petris again.

before anyone pops up, and suggests
" ability to work with the openings impairs"
of course it does if ya lack any sense of imagination.

theres a screwdriver , for every occasion with polycarbs,
and oddly, screw drivers work better for agar, than exacto knives!!!!

experience, leads me to suggest,
one can outrun petri agar farms with polycarbs, based on contams, handling and size.

huh? what handling?
if your a 3 dish farm , no one cares,
however, for the larger agar farms,
stacking any serious amount of petris is a farce at best.
my op is tight, i dont grow sitcom style.
polycarbs stack right, tight, neat and clean every time

" to each their own" shouldnt be suggested to encourage any low standard, or allow for mediocrity as a norm.
" to each their own" should be in context, that all strive for excellence.


in the new age, expect more,
for you ,your grow, and your equipment.


--------------------
:aliendance:


Edited by anne halonium (11/28/13 05:05 PM)


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OfflinecronicrFacebook
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
    #19201646 - 11/28/13 05:16 PM (10 years, 2 months ago)

just try them all or pick one, whatever gets you your clean culture that's the point:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleanne halonium
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Registered: 05/07/13
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Re: Glass vs Plastic petri dishes, cant decide [Re: cronicr]
    #19201677 - 11/28/13 05:23 PM (10 years, 2 months ago)

try em all, and realize wich ones give capability and usability best.
and then apply proper sterile skills to effect.

i hope cronic , wasnt suggesting that,
you based your choice on sterile luck values and uncertain skills.
because, pros can get a clean culture in anything.

dont spin " whatever works" into " what works best"
if thats the contest, hell, use pony beer bottles.


--------------------
:aliendance:


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