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Gloogloos
Stranger

Registered: 01/28/12
Posts: 63
Last seen: 6 years, 3 months
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Glass vs Plastic petri dishes, cant decide
#19200675 - 11/28/13 12:41 PM (10 years, 2 months ago) |
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New to agar culturing here, so im trying to decide on petri dishes, glass or plastic (or even those little ball jars).
Glass - I like the idea of high quality, reusable equipment because A. I don't have to worry about getting new ones all the time and B. I can sterilize them myself. But is it worth the cost and is it worth the fragility of them?
Plastic - cheap and readily available. I would rather not have to always ordering them though, and I don't trust packaging to keep them sterile, plastic sleeves tear easily. Plastic also will let me dick around with different sizes and dividings though, which would be nice.
Jars! - I love jars, and those little 1/4 pint jars seem like they could work. The feedback seems positive, people like them because they are relatively cheap, durable, available and you can sterilize agar in them, cutting out a step. However they seem too deep and they seem like they could be difficult to cut wedges out of and they seem like they would be space consuming if you were doing like 50 or more.
Any feedback would be greatly appreciated. My current idea is to get those jars for this fist attempt, as I feel they are less likely to get contaminated, I can get them locally and I am only doing maybe 10-20 dishes this first time. I figure I can use these as master cultures and then get disposables for isolating strains and growing cultures to move to grain jars.
Thank you thank you ahead of time.
TLDR: GLASS vs PLASTIC vs 1/4 pint JARS
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Glass vs Plastic petri dishes, cant decide [Re: Gloogloos]
#19200713 - 11/28/13 12:50 PM (10 years, 2 months ago) |
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You can re-use glass petris but you still need to sterilize them empty, let them cool, then pour your agar.
You might imagine that you can load the glass petris with your media and then PC them but this is not the case, the agar will just boil over everywhere.
No pour jars are better than glass petris.
But almost every experienced cultivator I've ever talked to uses regular plastic petris, myself included.
Because they are small, easy to work with, and perfect for the task
Edited by FrankHorrigan (11/28/13 12:58 PM)
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: Glass vs Plastic petri dishes, cant decide [Re: FrankHorrigan] 1
#19200730 - 11/28/13 12:55 PM (10 years, 2 months ago) |
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I'm using these things:
http://www.amazon.co.uk/GSL%C2%AE-PLASTIC-STORAGE-CONTAINERS-Weekdays/dp/B005VB50W6/ref=pd_sim_sbs_kh_2
(although not form amazon - just showing the product)
PCable...seem pretty perfect for no pour 
(but then I am an agar noob - seems to be working out so far )
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: Skinty]
#19200827 - 11/28/13 01:17 PM (10 years, 2 months ago) |
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Gloogloos there's an option you forgot. Plastic no-pour agar dishes No regular orders online, no wasted plastic, reusable, no carefully-timed pour procedure, high sterile success, no parafilm, no loose "lids" (petri tops), wide grow-out area... ... and of course a nearly limitless variety of uses! The only thing they aren't great for is long-term refrigeration due to their height.
Plastic disposed petris Suck IMHO. People keep using them not because they're somehow actually the best option but because they're cheap at face value. However despite their lack of monetary expense, the price of such reckless plastic waste is greater. 5-10¢ for a sterile plastic dish is an artificially low value.
If you use a recycling code 5 plastic screw-top container as such 8-12 times then it's paid for itself and used only a fraction of the plastic, with still more uses to come!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19200872 - 11/28/13 01:27 PM (10 years, 2 months ago) |
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I still like regular plastic petris. I use the 60mm ones.
Glass dishes are better for things like crystallization. They're too heavy and slippery to really use for our purposes.
I am completely against the idea of those plastic reusable containers. A wide area to grow out isn't an advantage. It allows much more room for contams. They also use much more agar than needed, and you can't ship them if you wanna trade a culture. You can barely see through them to spot contams. Just not worth it IMO.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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dusttodust


Registered: 11/26/12
Posts: 491
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19200937 - 11/28/13 01:45 PM (10 years, 2 months ago) |
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Truth to tell, i do thing opposite from what Frank said.
I have 6 glass dishes. One cost around 3€. Some argue that those are cheap dishes and will break but i have been using them for some time and they havent broken yet, im satisfied with them 
I normally pour them before i pc them: After boiling i let them cool to room temperature and i put them in baking bags and than i make knot at the end and i try to squeeze all the air out first of course, than i pc. Its a bit tricky and you have to get some experience first so you dont mess things up, but i find my method very easy and i dont have any problems with it.
I also sometimes pour after i sterilize. Well, its up to how i feel i guess.
I use glass when working with small projects and i can reuse them as many times as i want. If i need to clean up culture id rather use glass than waste many plastic ones.
If i had bigger projects than i would use plastic. Looks more handy and much cheaper.
I also use jars sometimes if i dont have any spare glass dish and if i dont want to open sleeve of plastic ones but its my last resort, i dont really like it.
I think Violets plastic-no-pour tek is also great and cheap and very useful.
There is no rule here, u can use anything as long as its sterile and it works for you. I suggest that if you are beginer, stick to verified methods.
Good luck!
Edited by dusttodust (11/28/13 03:10 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: dusttodust]
#19201165 - 11/28/13 03:01 PM (10 years, 2 months ago) |
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I get 500 plastic dishes for like $70 and I'm good for a year.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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TheCyndicate
Conglomerate



Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 8 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201219 - 11/28/13 03:13 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: I get 500 plastic dishes for like $70 and I'm good for a year.
Hey hack's you get them locally ? Or online?
Cyn
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: TheCyndicate]
#19201221 - 11/28/13 03:14 PM (10 years, 2 months ago) |
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Online...ebay or amazon....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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TheCyndicate
Conglomerate



Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 8 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201237 - 11/28/13 03:19 PM (10 years, 2 months ago) |
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Cyn
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: dusttodust]
#19201248 - 11/28/13 03:22 PM (10 years, 2 months ago) |
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Quote:
dusttodust said: Truth to tell, i do thing opposite from what Frank said.
not opposite in my case, but more advanced. we grow 2013 style , not 1953 cold war tech style. we us polycarbonate culture bottles. all the advantages of glass and plastic combined, and ,it avoids many of the downsides, of both.

glass or plastic petris, is not the real choice. polycarb bottles are.

embrace the future of grow equipment. order some today!
Quote:
dusttodust said: I suggest that if you are begginer, stick to verified methods.
i agree. problem is who verifys? modern growers, or amish?
if this held true always, everyones first car would be a horse.
modern labs have used culture bottles for some time now. in spite of modern lab advances, many still dont realize advanced equip is cheap and plentiful.
dont let the community standard, be your outdated standard. and dont let a fetish for "tradional" become a trap for "outdated"
dont let your grow, stop evolving, cuz other growers fear to evolve.
--------------------
Edited by anne halonium (11/28/13 03:37 PM)
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201264 - 11/28/13 03:27 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: I get 500 plastic dishes for like $70 and I'm good for a year.
14 cents per dish. At that price, I can use a container only 7 times and beat that value, while of course still having the container for more countless runs.
70 bucks buys 70 or more containers. 70 containers used only 8 times as dishes makes for 560 dish uses. And after all those containers' uses, while your 500 disposed dishes are in the garbage, there are still more container uses to come not only as "petris" but myriad other uses as well!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19201287 - 11/28/13 03:33 PM (10 years, 2 months ago) |
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To each their own.....I like my disposable dishes better, even tho I could get more uses out of other methods, I choose this method.
They take up less space, they are easier to handle IMO....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201304 - 11/28/13 03:37 PM (10 years, 2 months ago) |
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A single petri may take less space than containers when they're not stacked, but is Definitely not easier to handle. Screw-on lids instead of loose lids with parafilm, and no pours. And 70 containers sure don't take up as much space as 500 petris. So it depends on how you consider it. It takes such selective considerations to think disposed plastic is better. But as you said, "to each their own" inefficiency and waste...
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19201417 - 11/28/13 04:14 PM (10 years, 2 months ago) |
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Quote:
Violet said: But as you said, "to each their own" inefficiency and waste...
Inefficiency? Seems very efficient to me....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/28/13 04:16 PM)
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201442 - 11/28/13 04:20 PM (10 years, 2 months ago) |
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Throwing away 1000 pieces of plastic the size of drink coasters sounds efficient to you? Especially instead of re-using 1/8th of the plastic more than 50 times over? Maybe you don't mean "efficiency" like I do then.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: PussyFart]
#19201495 - 11/28/13 04:30 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: To each their own.....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: FrankHorrigan]
#19201555 - 11/28/13 04:49 PM (10 years, 2 months ago) |
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Quote:
FrankHorrigan said: But almost every experienced cultivator I've ever talked to uses regular plastic petris, myself included.
*you should get out more frank. modern labs use culture bottles wholesale these days.
i think your refering to just the growers, you know, who have limited pro lab experience. hardly a sample of what goes on globally in biotech.
fact is, polycarb culture bottles , are a excellent choice. for the informed.

frank always suggests, whatever he does is standard. sometime it is , sometimes its only his POV.
my standard, is rise to achieve "cutting edge." yours should be also. following the pack, is not leading the grow. its just replicating, and replicating , always winds up outdated.

500 ml polycarb culture bottles, each one has 50 sq cm agar.
no slopping, no breaking, no polyfilm , easily decontaminated, silent, easily stored , and long lasting. they pour and PC in one shot.
i used both glass and plastic dishes in 80's and 90's culture bottles contam lower rate IMO by a margin. unless theres a specific need, i never intend to use petris again.
before anyone pops up, and suggests " ability to work with the openings impairs" of course it does if ya lack any sense of imagination.
theres a screwdriver , for every occasion with polycarbs, and oddly, screw drivers work better for agar, than exacto knives!!!!
experience, leads me to suggest, one can outrun petri agar farms with polycarbs, based on contams, handling and size.
huh? what handling? if your a 3 dish farm , no one cares, however, for the larger agar farms, stacking any serious amount of petris is a farce at best. my op is tight, i dont grow sitcom style. polycarbs stack right, tight, neat and clean every time
" to each their own" shouldnt be suggested to encourage any low standard, or allow for mediocrity as a norm. " to each their own" should be in context, that all strive for excellence.
in the new age, expect more, for you ,your grow, and your equipment.
--------------------
Edited by anne halonium (11/28/13 05:05 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#19201646 - 11/28/13 05:16 PM (10 years, 2 months ago) |
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just try them all or pick one, whatever gets you your clean culture that's the point
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: cronicr]
#19201677 - 11/28/13 05:23 PM (10 years, 2 months ago) |
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try em all, and realize wich ones give capability and usability best. and then apply proper sterile skills to effect.
i hope cronic , wasnt suggesting that, you based your choice on sterile luck values and uncertain skills. because, pros can get a clean culture in anything.
dont spin " whatever works" into " what works best" if thats the contest, hell, use pony beer bottles.
--------------------
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5] 1
#19201681 - 11/28/13 05:25 PM (10 years, 2 months ago) |
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Quote:
36fuckin5 said: I am completely against the idea of those plastic reusable containers. A wide area to grow out isn't an advantage. It allows much more room for contams. They also use much more agar than needed, and you can't ship them if you wanna trade a culture. You can barely see through them to spot contams.
All complete garbage.
A wide grow-out area is an advantage, requiring fewer dishes and giving each more inoc power. "More room for contams" is folly. You need proper sterile tech regardless. If anything, your point ensures there's plenty of room to isolate from any potential contam without touching it.
They take only 20-25 mL per container, meaning 500mL of agar still makes 20+ dishes just like pouring petris. You must have no actual experience in this, nor have done the math at least.
If you want to trade a culture, use something else, such as wedges like most people. Many people think fragile cheap plastic dishes are a shit way to ship also anyways.
I can so easily see growth and contams in my CLEAR plastic containers. Duh.
Your claim is plain-old-fashioned bullshit from someone who clearly doesn't really know. Thus why you chose to speak on this is baffling.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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TheCyndicate
Conglomerate



Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 8 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#19201682 - 11/28/13 05:25 PM (10 years, 2 months ago) |
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Quote:
anne halonium said:
Quote:
FrankHorrigan said: But almost every experienced cultivator I've ever talked to uses regular plastic petris, myself included.
*you should get out more frank. modern labs use culture bottles wholesale these days.
i think your refering to just the growers, you know, who have limited pro lab experience. hardly a sample of what goes on globally in biotech.
fact is, polycarb culture bottles , are a excellent choice. for the informed.

frank always suggests, whatever he does is standard. sometime it is , sometimes its only his POV.
my standard, is rise to achieve "cutting edge." yours should be also. following the pack, is not leading the grow. its just replicating, and replicating , always winds up outdated.

500 ml polycarb culture bottles, each one has 50 sq cm agar.
no slopping, no breaking, no polyfilm , easily decontaminated, silent, easily stored , and long lasting. they pour and PC in one shot.
i used both glass and plastic dishes in 80's and 90's culture bottles contam lower rate IMO by a margin. unless theres a specific need, i never intend to use petris again.
before anyone pops up, and suggests " ability to work with the openings impairs" of course it does if ya lack any sense of imagination.
theres a screwdriver , for every occasion with polycarbs, and oddly, screw drivers work better for agar, than exacto knives!!!!
experience, leads me to suggest, one can outrun petri agar farms with polycarbs, based on contams, handling and size.
huh? what handling? if your a 3 dish farm , no one cares, however, for the larger agar farms, stacking any serious amount of petris is a farce at best. my op is tight, i dont grow sitcom style. polycarbs stack right, tight, neat and clean every time
" to each their own" shouldnt be suggested to encourage any low standard, or allow for mediocrity as a norm. " to each their own" should be in context, that all strive for excellence.
in the new age, expect more, for you ,your grow, and your equipment.
I just wanna know one thing . How are you able to extract a wedge ...say like in front of a flow hood ...with the only opening being the lid . I always try to keep the clean air blowing in to the culture. So I would imagine this would be difficult doing in this situation. And also those bottles have a lot unused surface area . They also look like they are some what bulky . Just my observation.
Cyn
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#19201687 - 11/28/13 05:26 PM (10 years, 2 months ago) |
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 give it a rest already nobody cares about your lab standards they don't scare anyone i'm sure there's labs all over the world using glad tupperware containers
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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dusttodust


Registered: 11/26/12
Posts: 491
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Re: Glass vs Plastic petri dishes, cant decide [Re: cronicr]
#19201722 - 11/28/13 05:36 PM (10 years, 2 months ago) |
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I think most people here are involved with recreational mycology, thats why advanced aproach may not be the best option.
Petris use far less space than bottles, right?
I think that any container that is sterile can be used, diversity is beautiful. Thinking in many diffrent ways bring new inventions and pushes science forward. And when u criticize Frank, i have to tell you that he taught me to dunk grains. He made an important step in mycology and I wouldnt call him old fashioned.
Even if polycarbonate is better than other materials, the diffrence IMO isnt so big. Glass, plastic or polycarbonate petri is still petri dish nevertheless. But i will look into polycarbonate material, it sounds interesting.
Quote:
Notahacker420 said: To each their own.....
Quote:
cronicr said: just try them all or pick one, whatever gets you your clean culture that's the point
Edited by dusttodust (11/28/13 05:40 PM)
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: cronicr]
#19201760 - 11/28/13 05:47 PM (10 years, 2 months ago) |
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they are actaully easy to use. they can be had in any size. i like the .5 litre ones, cuz a few containers , can make gallons of slurry in seconds. gallons, and i mean seconds, pop lids , add sterile water and shake.
think about it, millions are made every yr for biotech. they are DESIGNED as culture bottles. by pros , for pros. simply master the skills like the pros that use em. you act like that would kill ya.
cornicr, i worked in university labs before you were born. pro biotech workers have standards. you do not. and it shows , in your posts.
it also shows, in your inexperience of various lab culture contianers, and most of all, you have the option of offering sound advice on agar,and its handling , but ,you choose to simply make assumptions of who does what where.
im pretty confident, from all your posts ive seen, no serious biotech company would let you in the door, therefore, its no surprise, standard modern lab items, cause you such worry.
my work here is done...... youll all see more in annies " annie does agar" thread...... coming soon.
see ya there cronic.
--------------------
Edited by anne halonium (11/28/13 06:12 PM)
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Valyr
Con nulla non si fa nulla.


Registered: 05/08/13
Posts: 381
Last seen: 6 years, 9 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19201769 - 11/28/13 05:51 PM (10 years, 2 months ago) |
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Quote:
Violet said:
Quote:
36fuckin5 said: I am completely against the idea of those plastic reusable containers. A wide area to grow out isn't an advantage. It allows much more room for contams. They also use much more agar than needed, and you can't ship them if you wanna trade a culture. You can barely see through them to spot contams.
All complete garbage.
A wide grow-out area is an advantage, requiring fewer dishes and giving each more inoc power. "More room for contams" is folly. You need proper sterile tech regardless. If anything, your point ensures there's plenty of room to isolate from any potential contam without touching it.
They take only 20-25 mL per container, meaning 500mL of agar still makes 20+ dishes just like pouring petris. You must have no actual experience in this, nor have done the math at least.
If you want to trade a culture, use something else, such as wedges like most people. Many people think fragile cheap plastic dishes are a shit way to ship also anyways.
I can so easily see growth and contams in my CLEAR plastic containers. Duh.
Your claim is plain-old-fashioned bullshit from someone who clearly doesn't really know. Thus why you chose to speak on this is baffling.
Your just being mean. And not only in that post either lol.
You too annie lol. Though I am looking forward to that tek . I like the idea of the bottles, large area, readily available, reusable, pcable.. Only thing is I have no idea how id work through that bottle neck lol. If I can see it done I may buy some to see if I can do it too.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: Valyr]
#19201799 - 11/28/13 05:57 PM (10 years, 2 months ago) |
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last word for me. think of it as tough love from auntie annie.
i have direct, long term experience , with all sorts of petris, and all sorts of culture bottles, both professional,and ad hoc. i just gave ya the report, with pictures.
anyone, with similar experience, and pics, is welcome to make their case.
i tell you guys this stuff, because i want every grower, to be their best. thats it.
up to date info, and direct experience reports, is how everyone wins.
oh , and naturally, everyone is entitled to an opinion. those who combine an opinion, and attempt to defame me, had better know exactly what they are talking about............ im well known for swatting grow trolls, like fungal gnats.
in the new age.
--------------------
Edited by anne halonium (11/28/13 06:07 PM)
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet] 1
#19202258 - 11/28/13 07:26 PM (10 years, 2 months ago) |
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Quote:
Violet said:
Plastic disposed petris Suck IMHO. People keep using them not because they're somehow actually the best option but because they're cheap at face value. However despite their lack of monetary expense, the price of such reckless plastic waste is greater. 5-10¢ for a sterile plastic dish is an artificially low value.
Spare us. The amount of plastic petri dishes a busy cultivator throws out in a year is less than most people toss out in soda bottles every single day.
Petri dishes perform best when shallow and easy to work in, so that leaves plastic or glass. Glass costs much more originally, and costs again and again in energy and cleaning costs to sterilize for re-use. Ditto for pp5 containers. You also have to keep them sterile until pouring. Keep those kinds of things in mind when deciding. I prefer pre-sterilized plastic dishes. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 14 hours, 46 minutes
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Re: Glass vs Plastic petri dishes, cant decide [Re: RogerRabbit]
#19202347 - 11/28/13 07:48 PM (10 years, 2 months ago) |
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I always used the little wide mouth ball jars and an SFD on them.
It makes it hard to see the myce from the top but a flash light from
underneath seems fine IMO. I found it convenient to mix PDA right in
the jar enough to cover the bottom then pressure cook for 15 mins 15psi
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TheCyndicate
Conglomerate



Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 8 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: TheCyndicate]
#19202380 - 11/28/13 07:57 PM (10 years, 2 months ago) |
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Quote:
I just wanna know one thing . How are you able to extract a wedge ...say like in front of a flow hood ...with the only opening being the lid . I always try to keep the clean air blowing in to the culture. So I would imagine this would be difficult doing in this situation. And also those bottles have a lot unused surface area . They also look like they are some what bulky . Just my observation.
Cyn
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Glass vs Plastic petri dishes, cant decide [Re: TheCyndicate]
#19202551 - 11/28/13 08:48 PM (10 years, 2 months ago) |
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i will gladly see you there annie no worries, all i'm saying is each of what was discussed has it's ups and downs but all have the ability to get the job done which is the important thing and i wannna know what one the op chooses, you just see what you wanna see but thats ok nuthing wrong with being a little ocd
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Glass vs Plastic petri dishes, cant decide [Re: cronicr]
#19202596 - 11/28/13 09:04 PM (10 years, 2 months ago) |
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I only got one question for Annie and its sincere. How the hell do you get a scalpel in those bottles 
As for me, while I love me some Y and X plates, they are easier to work in for sure, I don't love the price, or the cost of parafilm for that matter (tried using saran and it just didn't feel right). For me no pour pp5 glad mini rounds do the lions share of the work. The mini rounds are small, the pop top is way easier to deal with in a SAB than screw tops. But that being said plastic petris look the best in pictures and are super easy to handle. I like both, depending on what im doing with them.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Glass vs Plastic petri dishes, cant decide [Re: Pastywhyte]
#19202646 - 11/28/13 09:19 PM (10 years, 2 months ago) |
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Quote:
Pastywhyte said: I only got one question for Annie and its sincere. How the hell do you get a scalpel in those bottles 
If you'd read, she uses a screwdriver. IDK how, it's some wingnut shit, but that's how it goes and apparently it works for her.
I'll stand by my previous statement, and my main argument, that there is WAY too much exposed agar. I use 60mm plates because to reach the center, I never have to put any part of my scalpel handle over the dish. Every single molecule, plus another quarter inch of my blade, which is flame sterilized, will not cover the radius of my petri, which allows me to only ever have flame-sterilized metal directly over my petri.
Violet, IDK where you grow, but I can guarantee that your spore count isn't higher than mine. I have a shitload of live flora around me and an awful Aspergillus problem. I would not trust a goddam thing that hasn't been heated to red hot to be over my exposed agar for a millisecond. It's a recipe for disaster in my environment.
Urban growing is all fine and dandy, but a lot of us live in the middle of nowhere where we don't necessarily have a decent environment to grow in or the thousands to spend that it takes to isolate a clean room. We live around life. That life produces pollen and spores. We have to work with that.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19202720 - 11/28/13 09:41 PM (10 years, 2 months ago) |
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Quote:
36fuckin5 said:
Quote:
Pastywhyte said: I only got one question for Annie and its sincere. How the hell do you get a scalpel in those bottles 
If you'd read, she uses a screwdriver. IDK how, it's some wingnut shit, but that's how it goes and apparently it works for her.
Whoa calm down thar. Guess you never misread a post before. Maybe instead of "harder than your husband" your title should read "bitchier than my wife" 
Regardless whether she's using a screwdriver or a rusty hanger I don't see how you would have much control reaching in there
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Glass vs Plastic petri dishes, cant decide [Re: Pastywhyte]
#19202757 - 11/28/13 09:50 PM (10 years, 2 months ago) |
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Quote:
Pastywhyte said:
Quote:
36fuckin5 said:
Quote:
Pastywhyte said: I only got one question for Annie and its sincere. How the hell do you get a scalpel in those bottles 
If you'd read, she uses a screwdriver. IDK how, it's some wingnut shit, but that's how it goes and apparently it works for her.
Whoa calm down thar. Guess you never misread a post before. Maybe instead of "harder than your husband" your title should read "bitchier than my wife" 
Apparently you haven't either. I took no offense. Tone of voice is everything, and is hard to convey in text. 
Quote:
Regardless whether she's using a screwdriver or a rusty hanger I don't see how you would have much control reaching in there 
You could have plenty of control with a steady hand. But working with a flowhood or a SAB, flaming outside of your sterile flow, you could not possibly keep enough of your tool red-hot to cover your entire amount of exposed agar without burning yourself.
I want enough of my tool red-hot to reach over my intended inoculation point by at least 1/4". So if I'm using a 60mm plate, I want 75 mm of my tool to be red hot before it enters my sterile airflow. That way I can have no doubt about the sterility of the part of my tool that matters.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19202769 - 11/28/13 09:53 PM (10 years, 2 months ago) |
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Don't you look at me in that tone of voice!
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19202770 - 11/28/13 09:53 PM (10 years, 2 months ago) |
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Quote:
RogerRabbit said:
Quote:
Violet said: Plastic disposed petris Suck IMHO. People keep using them not because they're somehow actually the best option but because they're cheap at face value. However despite their lack of monetary expense, the price of such reckless plastic waste is greater. 5-10¢ for a sterile plastic dish is an artificially low value.
Spare us. The amount of plastic petri dishes a busy cultivator throws out in a year is less than most people toss out in soda bottles every single day.
Wow RR, I'm extremely surprised to hear such environmental apathy from you.
How can such a point truly make sense without spin, justifications, double-standards? Does a huge waste of plastic by morons make a seemingly lesser plastic waste okay and excused? Should someone get away with theft on the basis that a rape is being committed somewhere else right now?
Quote:
RogerRabbit said: Glass costs much more originally, and costs again and again in energy and cleaning costs to sterilize for re-use. Ditto for pp5 containers.
Oh you bet that's true for glass.
Not true for pp5 containers. They pass heat extremely quickly. I cook 20 pp5 agar dishes in a 16qt cooker for 25-30 minutes, 5-10 minutes less than a quart jar with that 500mL of agar. A set of 20 disposed petris requires a 30-40 minute cooker run for its agar, and still requires the pour to boot!
Besides, the energy that went into the PRE-STERILIZED dishes at all stages ought not be forgotten, and is only utilized ONCE before disposal.
Usually scooping/knocking the old agar out is enough to prepare them for loading the next round.
Quote:
RogerRabbit said: You also have to keep them sterile until pouring.
You must be joking. "No-pour agar containers"??? Or did you mean this about the pre-sterilized disposed petris? That's the only thing it applies to. Thanks for adding another very valid point to my list of comparisons.
The sterilized pre-poured plastic containers for agar don't require a pour. Only being allowed to cool - which they can do inside the cooker by-the-way instead of having to time pouring a jar of agar before gelled at all, another noteworthy point. Sterile success chance is greater, and none are ever lost without being used. I never sit around waiting for 2 days to find out if any -and if so how many- of the carefully hand-poured plastic disposed dishes are going to go into the garbage contamm'd without even being used, due to the pour step. I sure used to before I fully realized that disposed dishes are Lame.
Anyone else want to try their hand at explaining how single-use disposed un-cookable plastics are equal to or greater than sterilizable reusable ones?
Quote:
36fuckin5 said: Violet, IDK where you grow, but I can guarantee that your spore count isn't higher than mine. I have a shitload of live flora around me and an awful Aspergillus problem.
Whaddya know, me too! Down to the Aspergillus problem. Might have to reconsider your guarantee. I could hardly grow anything here before switching to all-sterile. Most 'bulks' including PF cakes usually contam'd before second flushes. Yep that's right, even fully-colonized substrates. If anything I'd rather bet that your live spore count isn't as high as mine, unless you live in the same kind of environment as I do, which based on your description is quite possible.
By the way, about your "reaching" concerns over agar... When I begin my sterile work, I pull my scalpel out of an aluminum foil 'sleeve' that I sterilized the scalpel in along with whatever the sterile work is being done on. The whole tool has thus been sterilized, and I flame only the blade of the tool itself to prevent cross-contamination. I have an over 99% sterile rate, seeing 1 mold contam and 1 bacterial contam in the last about 250 transfers I've done, despite a wide area per dish ;]
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
Edited by Violet (11/28/13 10:05 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19202828 - 11/28/13 10:08 PM (10 years, 2 months ago) |
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Quote:
36fuckin5 said:
Quote:
Pastywhyte said:
Quote:
36fuckin5 said:
Quote:
Pastywhyte said: I only got one question for Annie and its sincere. How the hell do you get a scalpel in those bottles 
If you'd read, she uses a screwdriver. IDK how, it's some wingnut shit, but that's how it goes and apparently it works for her.
Whoa calm down thar. Guess you never misread a post before. Maybe instead of "harder than your husband" your title should read "bitchier than my wife" 
Apparently you haven't either. I took no offense. Tone of voice is everything, and is hard to convey in text. 
No worries, I just been drinkin too much and pissed that I can't smoke weed for another 2 weeks
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19202833 - 11/28/13 10:10 PM (10 years, 2 months ago) |
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I'm just gonna say, IDK how much you grow, I know how much Anne grows. We've talked about it personally. With the efforst she puts in over 3 months, I did the math and I double her yield and I do it cheaper. I know how much RR grows, at least for Shiitakes. I'm in awe. I have a good idea of how much FH grows and I'm in awe. I have a good idea of how much NAH grows, and it probably matches mine.
You derive your ideas from Anne. I've done the math, and I do better than her, even with my contam problems.
I'm done here. Any serious pros will side with me and RR. Anne is the only person I know of with years of experience that will argue. IME, if you don't have 5-10 years of experience, you haven't dealt with every situation that will come up, and even in that time frame, unless you try to grow in some FUCKED UP places like I have, you won't see all the contams you could. I respect Anne's arguments, because I know she's been at it forever. Way longer than me. I don't agree with her methods for everyone, but I believe they live up to her standards.
Violet, while I respect your opinion, I have to keep in mind how many agar transfers you could have possibly done in 2 years as opposed to how many some of us older people have done it in 10+ years. I've been growing a LOT of mushrooms for a LOT of years in a LOT of different environments. I've had lots of time to figure out what works everywhere and what is environment-specific.
Main point being, don't push something as gospel until you've produced multiple hundreds of pounds in multiple locations. Every environment is different, and mushroom farming IS NOT A SCIENCE yet. As a whole, we really don't know shit. Nobody can write a gospel yet.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19202938 - 11/28/13 10:37 PM (10 years, 2 months ago) |
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Mushroom growing is an extremely fun hobby for me and I love playing with it in all kinds of ways. However I have some aspects of my life where I have aspired to mastercraft-quality work and/or innovations to style. I did such in less time than most people of my caliber spent just going to school for those things alone. A conversation started by a veteran asking how I got my practise as such with so little time quickly ended when he couldn't follow my answers, making evident that I learned more in two years of experience and practise than he did in fifteen. The drive to learn and improve put it on a point of priority for me to where I simply learned more than he'd made a point of over far longer. And my style of mind was always keen to learn something in any given moment of the practises where others became content to repeat the simple processes of the learned labor. All the time I get set-in-their-ways old hands use known inferior practices, grunting "I've been doing this 30 years" as their defense when it's addressed, speaking to me as if it should have already been known, given, that they are not in question about that.
I don't say that to be egotistical. That didn't make me "better" than that man any more than his fifteen years would have made him "better" than me were it not just shown otherwise. I say it to remind you not to pander to years of experience itself carrying more weight than it truly does. Not saying you did that, just that your statements come line-toeing close to that fallacy. I'm not saying I'm better than you. If you're so unwise to say you're better than me, with that as your excuse, I'll get to protesting. Besides, I've been at home myco longer than I've had this Shroomery account. (Not years longer tho)
So in following suit with you, I've seen how all those people grow too, and impressed as I am with their success, after fully experiencing the bulk teks I first learned here I personally found them lacking or troublesome or inefficient. Short fact is I do far more now with far less, and am not fighting contams or environment or disposal or whatever anymore. It's all a breeze, growing like a dream. Sometimes it's even a little hard to believe that growing so powerfully and effectively can be so small and simple.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19203002 - 11/28/13 10:56 PM (10 years, 2 months ago) |
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Fair enough, but I'll say keep this account for 15 years, then tell me what you've learned.
Sometimes aggravation from people refusing to learn from your mistakes, instead of making those mistakes themselves, get to you, especially when you've been there, done that, and couldn't affor the T-shirt cause your whole project failed.
If it works for you, do it. But I'm telling you, I've done way more than I admit on here, and if you don't notice I don't produce pics or admit yields over the long-term. Yes, waste can be a problem, and you've gotten mostly around that, but looking at it dollar-in to dollar-out, even charging minimal prices (cause I only sell half-legit edibles to local shady restaurants) my methods make the most sense.
I get experimentation, but you seem to have done one set of experiments and got set in your ways. I've had experimentals tubs, trays, bags, Tupperware, etc going for the last decade, and still do. I get set in my ways because they're the most efficient for me, and work in every single environment. I always still have 1 or 2 experimental tubs at all times.
Again, growing mushrooms is not a real science yet. What works for you works for you. But my methods work reliably for everyone, every time.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#19203056 - 11/28/13 11:11 PM (10 years, 2 months ago) |
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Quote:
36fuckin5 said: Fair enough, but I'll say keep this account for 15 years,
Wish I could but unfortunately with the continuing revelations about the changes of the digital era it will be a surprise if I upkeep this account another whole year, and honestly I suggest as much for many people here.
Past that, I agree, "Fair enough"!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: Violet]
#19203624 - 11/29/13 05:09 AM (10 years, 2 months ago) |
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thing is 36f, pros can do anything. if you and roger for instance, suggested about anything, id be like " cool". why? cuz you guys are informed experienced pros.
serious pros, really cant go wrong, because, they " garden engineered "it all with forethought.
pros can use pony beer bottles, and shoe boxes lined with plastic and rule. skill does indeed trump equipment.
thing is, not all growers are pros, and there seems to be 2 schools of grow. one school , is the lab growers , the other the barn growers. one type urban, one type rural. both have advantages and disadvantages.
in the case of the rural growers , both pro and otherwise, equipment needs may be more forgiving, as the dynanmic allows for more options, and operating space.
urban growers, have space, time, noise, smell , heat , energy and power considerations. equipment , can be a BIG deal to urban growers. it can make the difference in total crop and handling.
my teks, and the angle i take, is to enhance the urban lab grower, giving them the teks, to compete/ exceed with the rural bulkers .
once again, equip does not trump skill. however, best choices of equipment , make a BIG deal in ALL the factors, associated with an urban grow op.
LED light, makes unusual grow spaces possible, with low heat and low power, and more stealthy,
culture bottles, make labs durable, safe and silent, and solves many handling issues.
PP5, durable , safe, silent, stackable, cheap, and also easily handled.
AA cookers, durable and reliable.
blue light, low power, low heat , low IR. durability, stealth.
point is, equip matters to some. it can make a difference. id still be able to operate , as any pro would, if i didnt have anything but ad hoc basics, but, in an urban environment, proper equip, enables the mega grow, with way less hassle or downsides. certian equip, does make handling and stealth enhanced.
contrary to popular belief, im not out to convert bulkers. im out to enhance urban lab growers, and enhance small lab growers ops.
i dont recall ever telling anyone , how to run their barn grow. why would i? ...... what i dont get , why barn peeps, think they can tell me how to run my lab? its 2 different types of grows.
my thing, is about info. the informed ,,with lots of choices , have best grow engineering. no matter what environment or methods chosen.
bulkers, wont go extinct cuz of anything i ever do. they may find, however, that urban lab growers gain influence and clout, as new teks, and new equip options, become more common.
Quote:
Violet said: fact is I do far more now with far less, and am not fighting contams or environment or disposal or whatever anymore. It's all a breeze, growing like a dream. Sometimes it's even a little hard to believe that growing so powerfully and effectively can be so small and simple.
^ this, is what the team annie difference, is all about. realizing, the teks and equip, AND skill, can make a difference.
in the new age we should expect alot.
--------------------
Edited by anne halonium (11/29/13 03:27 PM)
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Gloogloos
Stranger

Registered: 01/28/12
Posts: 63
Last seen: 6 years, 3 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#19204116 - 11/29/13 09:21 AM (10 years, 2 months ago) |
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This is fun, a whole lot of ideas and opinions, thank you.
Here is another consideration about the disposables, you guys are arguing the worth of them, and I have been reading a lot of threads and posts discussing the effect of throwing away so many plastic dishes. Many say its irrelevant, I personally disagree, but, as RogerRabbit has said in many other threads, he donates them to schools and what not.
As an illegitimate mycologist, I can't just appear out of nowhere to a school and donate 100 plastic petri dishes on a fairly regular basis, its also weird for me to have those in my trashcan on a fairly regular basis.
With the reusable ones, be them dishes or jars or bottles or whatever, I don't have to worry about to disposing them, which is a huge plus for me and I am sure many others.
Just a thought, feel free to continue arguing, its all very educating.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Glass vs Plastic petri dishes, cant decide [Re: Gloogloos]
#19204195 - 11/29/13 09:47 AM (10 years, 2 months ago) |
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glad to be of assistance. information, education, awareness of choices, and adjusting to the grow environment at hand, is the key to it all.
operators are standing by.............
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pseeker
Morphing

Registered: 11/02/14
Posts: 11
Last seen: 7 years, 8 months
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#21015233 - 12/23/14 01:03 AM (9 years, 1 month ago) |
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we use plastic
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Glass vs Plastic petri dishes, cant decide [Re: pseeker]
#21015256 - 12/23/14 01:16 AM (9 years, 1 month ago) |
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ohokayigetit
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Glass vs Plastic petri dishes, cant decide [Re: 36fuckin5]
#21015295 - 12/23/14 01:39 AM (9 years, 1 month ago) |
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i like plastic. The glass are reusealble though?
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Glass vs Plastic petri dishes, cant decide [Re: mrbart4444]
#21015306 - 12/23/14 01:47 AM (9 years, 1 month ago) |
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yeah reusable means you have to clean them and re-esterilize them. :fuckthatshit:
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mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
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Re: Glass vs Plastic petri dishes, cant decide [Re: Mr. Alien]
#21015313 - 12/23/14 01:51 AM (9 years, 1 month ago) |
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Yea put they would be idea for a no pour.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 35 minutes
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Re: Glass vs Plastic petri dishes, cant decide [Re: Mr. Alien]
#21015621 - 12/23/14 06:37 AM (9 years, 1 month ago) |
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Quote:
Mr. Alien said: yeah reusable means you have to clean them and re-esterilize them. :fuckthatshit:
You make it sound like that's a lot of work. It takes maybe 5 seconds to clean out a dish and a couple more seconds to reach over and set it on the rack of my toaster oven.
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Glass vs Plastic petri dishes, cant decide [Re: Kizzle]
#21015756 - 12/23/14 07:51 AM (9 years, 1 month ago) |
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I was using pre-sterilized plastic petris and they worked great, the only draw back was I wasn't ordering enough to keep up with inoculations, transfers, etc.
I found 14 glass petris for cheap - like $12 USD, shipped. Though I'm down to about 10 after a couple shattered. Cleaning and sterilizing isn't a big deal, after cleaning, I wrap them in foil an PC them along with the agar bottle.
IMO, one really isn't much better than the other; though I think I'd be better off ordering 500 plastic petris and be good for a year, as PussyFart mentioned.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Glass vs Plastic petri dishes, cant decide [Re: Kizzle]
#21015773 - 12/23/14 07:55 AM (9 years, 1 month ago) |
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Quote:
Kizzle said:
Quote:
Mr. Alien said: yeah reusable means you have to clean them and re-esterilize them. :fuckthatshit:
You make it sound like that's a lot of work. It takes maybe 5 seconds to clean out a dish and a couple more seconds to reach over and set it on the rack of my toaster oven.

I remember this thread. It was fun.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 35 minutes
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Re: Glass vs Plastic petri dishes, cant decide [Re: mycomattie]
#21018881 - 12/23/14 08:15 PM (9 years, 1 month ago) |
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How did they shatter?
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Glass vs Plastic petri dishes, cant decide [Re: Kizzle]
#21019349 - 12/23/14 10:16 PM (9 years, 1 month ago) |
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Quote:
Kizzle said:
Quote:
Mr. Alien said: yeah reusable means you have to clean them and re-esterilize them. :fuckthatshit:
You make it sound like that's a lot of work. It takes maybe 5 seconds to clean out a dish and a couple more seconds to reach over and set it on the rack of my toaster oven.
Even after cleaning them... wasting energy in the PC just to re-esterilize them empty?
If I'm cleaning dishes after using them it better be a no pour method like Pastywhyte Tek or similar. Glass petri dishes don't save that much money if you take into account that you have to re-esterilize them empty after cleaning them.
They may be money savers if you have a big esterilizer were you can sterilize hundreds of them in one shot.
just my two cents... But yeah i'm lazy
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 35 minutes
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Re: Glass vs Plastic petri dishes, cant decide [Re: Mr. Alien]
#21019515 - 12/23/14 10:54 PM (9 years, 1 month ago) |
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Quote:
Even after cleaning them... wasting energy in the PC just to re-esterilize them empty?
Do them at the same as your jars. No extra energy needed then.
I like the toaster oven though. I can disinfect the surface they'll be sitting on in it at the same time not to mention the handles for my blades and inoculation loop. Now if I could just find some reusable glove that could stand up to dry heat sterilization I would be set
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Glass vs Plastic petri dishes, cant decide [Re: anne halonium]
#21019597 - 12/23/14 11:12 PM (9 years, 1 month ago) |
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Quote:
anne halonium said: glad to be of assistance. information, education, awareness of choices, and adjusting to the grow environment at hand, is the key to it all.
operators are standing by.............
thank god its just an old thread....my heart stopped for a sec
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Glass vs Plastic petri dishes, cant decide [Re: Kizzle]
#21020329 - 12/24/14 07:48 AM (9 years, 1 month ago) |
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Quote:
Kizzle said: How did they shatter?
Just from me mishandling them or dropping a lid or bottom.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 35 minutes
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Re: Glass vs Plastic petri dishes, cant decide [Re: Mr. Alien]
#21034021 - 12/28/14 03:01 AM (9 years, 1 month ago) |
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Quote:
Mr. Alien said:
Quote:
Kizzle said:
Quote:
Mr. Alien said: yeah reusable means you have to clean them and re-esterilize them. :fuckthatshit:
You make it sound like that's a lot of work. It takes maybe 5 seconds to clean out a dish and a couple more seconds to reach over and set it on the rack of my toaster oven.
Even after cleaning them... wasting energy in the PC just to re-esterilize them empty?
If I'm cleaning dishes after using them it better be a no pour method like Pastywhyte Tek or similar. Glass petri dishes don't save that much money if you take into account that you have to re-esterilize them empty after cleaning them.
They may be money savers if you have a big esterilizer were you can sterilize hundreds of them in one shot.
just my two cents... But yeah i'm lazy 
Based on this it could easily be a cent a piece or less to oven sterilize them.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Glass vs Plastic petri dishes, cant decide [Re: Kizzle]
#21034069 - 12/28/14 03:42 AM (9 years, 1 month ago) |
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Kizzle yeah i saw your tek about sterilizing petri dishes in the oven, pretty cool.
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dusttodust

Registered: 11/26/12
Posts: 491
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Re: Glass vs Plastic petri dishes, cant decide [Re: Mr. Alien]
#21034278 - 12/28/14 06:41 AM (9 years, 1 month ago) |
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I put my petris in dishwasher, than i bake them in my oven.
Ah, and btw, anne halonium was causing troubles in mycotopia too, some people were angry just by mentioning his username there lol
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