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OfflineTerry M
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What the HELL are these tendrils?
    #19196926 - 11/27/13 03:03 PM (10 years, 2 months ago)

At first, I thought they were pseudopodia from an amoeba. But this is a transfer done from the edge of the previous plate's mycelium, which also had the tendrils. It can't be a procaryote like an amoeba because I don't see how I couldn't have accidentally gotten a piece with the whole genome.



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Edited by Terry M (11/27/13 03:11 PM)


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OfflineKizzle
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Re: What the HELL are these tendrils? [Re: Terry M]
    #19196994 - 11/27/13 03:21 PM (10 years, 2 months ago)

Are they slimy? It could be bacteria


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OfflineTerry M
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Re: What the HELL are these tendrils? [Re: Kizzle]
    #19197026 - 11/27/13 03:29 PM (10 years, 2 months ago)

None of the usual bacterial slimy shininess. But they don't appear to be on the surface, but seem to like hugging the bottom of the agar. The second picture is the bottom of the plate.

Which now makes me think "anaerobic."


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OfflineMAIA
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Re: What the HELL are these tendrils? [Re: Terry M]
    #19197933 - 11/27/13 07:18 PM (10 years, 2 months ago)

Quote:

Terry M said:
It can't be a procaryote like an amoeba because I don't see how I couldn't have accidentally gotten a piece with the whole genome.





IMHO that is not a procaryote. With a procaryote in such situation, asexual reproduction would be achieved through binary fission and that occurs in the nucleus. Even if you're wondering if that's an amoeba, look at the size as well, I think that's over 8mm. That exceeds max size on amoebas.


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Edited by MAIA (11/27/13 07:31 PM)


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OfflineTerry M
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Re: What the HELL are these tendrils? [Re: MAIA]
    #19198720 - 11/27/13 11:05 PM (10 years, 2 months ago)

Slime mold? That's more like what I was thinking when I said amoeba. They have lots of nuclei packed in a single cell membrane, and can grow quite large.


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OfflineTerry M
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Re: What the HELL are these tendrils? [Re: Terry M] * 1
    #19202540 - 11/28/13 08:45 PM (10 years, 2 months ago)

The mystery is solved.

I looked at these things from the bottom of the Petri dish, and what a saw were bundles of mycelium! The important information I left out was that this is a culture of Armillaria mellea. The big tendrils are actually rhizomorphs, rootlike structures composed of mycelium. They are characteristic of Armillaria mellea.

This is a pure culture of A. mellea. That explains why all attempts to isolate the "contaminant" failed!

http://www.bioart.co.uk/lux/arm.html

Also this 9 year old thread:
http://www.shroomery.org/forums/showflat.php/Number/3349906


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Edited by Terry M (11/28/13 09:11 PM)


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