|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Delay
Stranger


Registered: 10/29/13
Posts: 331
Last seen: 2 years, 10 months
|
GLC during sclerotia formation
#19196810 - 11/27/13 02:37 PM (10 years, 2 months ago) |
|
|
Hi there,
I decided to make GLC from a sclerotia-producing species. I milked a GLC at 100% colonization, and I also milked one at ~3 months into sclerotia formation. When I inocculated substrate with the ~3 month GLC, I noticed that the inoculated substrate began forming stones immediately and colonized less rapidly. The growth resulting from GLC milked at 100% colonization, however, did not begin sclerotia formation until after it had fully colonized. It seems that mycelium used for GLC which is well into stone formation remains in a stage of it's life cycle that focuses more heavily on stone formation and less so on colonization, and does not fully revert to colonization. Is this indeed what might explain my observations.
|
dusttodust


Registered: 11/26/12
Posts: 491
|
Re: GLC during sclerotia formation [Re: Delay]
#19197074 - 11/27/13 03:46 PM (10 years, 2 months ago) |
|
|
Interesting, but if you didnt work with monoculture this information is not reliable, you know that right?
|
Delay
Stranger


Registered: 10/29/13
Posts: 331
Last seen: 2 years, 10 months
|
Re: GLC during sclerotia formation [Re: dusttodust]
#19197096 - 11/27/13 03:54 PM (10 years, 2 months ago) |
|
|
Good point, it was indeed from MS. I'll try this again with a monoculture. Nonetheless, it was a notable observation which I thought worth sharing. I was hoping others might have made similar observations and/or have some input as far as why I might have noticed this.
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
|
Re: GLC during sclerotia formation [Re: Delay]
#19197290 - 11/27/13 04:41 PM (10 years, 2 months ago) |
|
|
That's about what I would expect to happen. Sclerotia form when the myc realizes it's not going to get to fruit. They continue to grow the stones, but colonization pretty much slows to a crawl at that point.
Stretching out an old culture is never a good idea.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
Delay
Stranger


Registered: 10/29/13
Posts: 331
Last seen: 2 years, 10 months
|
Re: GLC during sclerotia formation [Re: 36fuckin5]
#19197369 - 11/27/13 05:03 PM (10 years, 2 months ago) |
|
|
Quote:
36fuckin5 said: That's about what I would expect to happen. Sclerotia form when the myc realizes it's not going to get to fruit. They continue to grow the stones, but colonization pretty much slows to a crawl at that point.
Stretching out an old culture is never a good idea.
I was under the impression that senescence wouldn't really matter in the span of only a few generations, so I didn't attribute the differences to age time-wise, but mostly life-cycle-wise.
I have two cultures with distinct growth patterns, both second generation, both from the same master jar, milked at separate times. I might be wrong, but I think you are speaking too broadly when you say "old". That could relate to time, to generations, or as I'm suggesting, to a given stage in a life cycle. In my case, both culture are the same age in terms of time, yet one performs well while the other is sluggish. I appreciate that time-wise it's better to propagate young cultures, but that's beside my point which is that the stage of a culture's life cycle might also affect vigor when propagating with respect to sclerotia-forming species.
When I've cloned tissue from a mushroom, the mycelium was quite strong and healthy, despite being excised from a culture which had practically abandoned colonization in favor of fruiting. For that reason I had the opposite expectations as you. I thought the culture would "realize" it has new material to colonize and revert to a stage in which it would do so.
Edited by Delay (11/27/13 07:39 PM)
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 527 topic views. 45 members, 245 guests and 56 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|