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OfflineCaptainpaps
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opinion on this agar section
    #19196736 - 11/27/13 02:18 PM (10 years, 2 months ago)

I'm in search of opinions on whether to use this section to make a master grain jar or not. thanks!



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OfflinecronicrFacebook
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Re: opinion on this agar section [Re: Captainpaps]
    #19196789 - 11/27/13 02:32 PM (10 years, 2 months ago)

i'd opt to take a quick transfer if thats a grain on there i see


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Invisiblemycomattie
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Re: opinion on this agar section [Re: cronicr]
    #19196808 - 11/27/13 02:36 PM (10 years, 2 months ago)

Wow - VERY rhizomorphic/ropey!


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OfflineCaptainpaps
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Re: opinion on this agar section [Re: cronicr]
    #19196862 - 11/27/13 02:48 PM (10 years, 2 months ago)

yep thats a grain on there, im just feeling froggy today, i have some transfers from this dish that are more on the rhizo/ropey side but its 3 days old and i know the bigger the chunk the faster colonization.


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Offlineflipsidetrue
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Re: opinion on this agar section [Re: Captainpaps]
    #19197097 - 11/27/13 03:54 PM (10 years, 2 months ago)

i'm no professional but i dont see why you wouldnt be able to use that for a master jar seeing that it has no contams, It looks healthy to me i'd use it :shrug:


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Edited by flipsidetrue (11/27/13 04:02 PM)


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OfflineCaptainpaps
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Re: opinion on this agar section [Re: flipsidetrue]
    #19197149 - 11/27/13 04:07 PM (10 years, 2 months ago)

i think i'll wait on more opinions before i jump into anything, just to be on the safe side, not saying you dont know your stuff or anything i would just feel better if others chimed in. :thumbup:


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Offlineflipsidetrue
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Re: opinion on this agar section [Re: Captainpaps]
    #19197172 - 11/27/13 04:14 PM (10 years, 2 months ago)

its all good, just trying to help goodluck!


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Invisible36fuckin5
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Re: opinion on this agar section [Re: flipsidetrue]
    #19197366 - 11/27/13 05:03 PM (10 years, 2 months ago)

You could use it, but it's best to make a transfer to 100% verify that it's clean. It likely didn't, but something could have transferred with that grain, contam spores could have been covered with good myc before they got to germinate and then when you throw it in a grain jar, they get their chance. No fun.

Do a transfer. At least one. I have the rule that only agar inoculated with agar can go in my jars. If I start a plate with tissue or spores or whatever, I always make at least one transfer.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineCaptainpaps
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Re: opinion on this agar section [Re: 36fuckin5]
    #19197524 - 11/27/13 05:46 PM (10 years, 2 months ago)

awesome i took your advice and used some transfers i had from that dish i just dropped some wedges in 1 jar, but i had a wedge fall on the bottom of the sab i still used it tho... should i be concerned? :ohgodpleaseno:


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Invisible36fuckin5
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Re: opinion on this agar section [Re: Captainpaps]
    #19197540 - 11/27/13 05:49 PM (10 years, 2 months ago)

Yes, that jar will likely contam.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineCaptainpaps
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Re: opinion on this agar section [Re: 36fuckin5]
    #19197637 - 11/27/13 06:14 PM (10 years, 2 months ago)

:feelsbadman:


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OfflineCaptainpaps
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Re: opinion on this agar section [Re: Captainpaps]
    #19198072 - 11/27/13 07:57 PM (10 years, 2 months ago)

is there a good chance that it wont contam?


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Invisibleblindingleaf
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Re: opinion on this agar section [Re: Captainpaps]
    #19199872 - 11/28/13 09:01 AM (10 years, 2 months ago)

50/50 dude.  if u already have dishes growing from transfers from that dish, use those, the ones u mentioned that are 3 days old. cut the myc out of it, leaving the outer edge of agar, like a pin wheel, or a third of a pin shell if using those 3 section plates


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MICROBIAL HUSBANDRY!!!!

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Invisible36fuckin5
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Re: opinion on this agar section [Re: blindingleaf]
    #19200847 - 11/28/13 01:21 PM (10 years, 2 months ago)

It's way less than a 50/50 shot. That jar is pretty much destined for failure.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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