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ballrawg
Stranger

Registered: 02/08/13
Posts: 52
Last seen: 8 years, 7 months
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first flush small
#19195731 - 11/27/13 10:16 AM (10 years, 2 months ago) |
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Want is it that i get a better yield with mature fruits on my second flush compared to my first flush. My first flush all my fruits are only 3-4 inches with tiny caps with the veil nowhere in sight. What am i doing wrong with my monos. I'm working with isolate from a 100g fresh mushie.
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
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Re: first flush small [Re: ballrawg]
#19195742 - 11/27/13 10:19 AM (10 years, 2 months ago) |
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You grew a 100g mushie?! Did you dunk and roll before fruiting(if you are working with cakes)? By isolate are you working with agar wedges or spores from the 100g mushie?
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ballrawg
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Re: first flush small [Re: SamhainJ]
#19195757 - 11/27/13 10:24 AM (10 years, 2 months ago) |
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I'm working with monotubs. My isolate from my 100 g mushie is a lc.
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Aero
Orea


Registered: 11/01/13
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Re: first flush small [Re: ballrawg]
#19195787 - 11/27/13 10:30 AM (10 years, 2 months ago) |
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whats the temp ? and beside that there are still so many factors playing, humidity, fae, nutritions..
-------------------- SPREAD THE SPORES
Edited by Aero (11/27/13 10:50 AM)
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ballrawg
Stranger

Registered: 02/08/13
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Re: first flush small [Re: Aero]
#19195896 - 11/27/13 10:56 AM (10 years, 2 months ago) |
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I'm thinking it might be fae but i fan it 3 times a day. Should every single mushie be as big as my isolate? Wtf?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: first flush small [Re: Aero]
#19195926 - 11/27/13 11:03 AM (10 years, 2 months ago) |
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Was your isolate source fruit found on a monotub? If so the time it took from spores to spawn to any G2g to the colonizing of coir to fruiting then take that fruit put it to agar then grow it out in a LC then that LC to more spawn and spawn to coir and then that flushing is a hell of a lot of cell divisions so you might have poor performance because of that. Otherwise any isolation you did from a clone fruit could possibly be a poor performer on its own. A clone fruit from MS grows can have quite a lot of genetics/strains that might work with eachother better than separately. I was told to just use clone tissue as clone tissue and get your isolates from spores for this reason in my own thread here worth a readthru
Edited by Trusted cuItivator (11/27/13 11:04 AM)
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Aero
Orea


Registered: 11/01/13
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Re: first flush small [Re: ballrawg]
#19195931 - 11/27/13 11:04 AM (10 years, 2 months ago) |
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even from a tissue, if u put it on agar, u can still see sectoring, which means it isnt an isolate yet, isolating these sectors to a monoculture and fruit every each of them will give a better result, u will see homogeneous fruits
and yet a clone in ideal conditions will more likely give look a like fruits which means it really depends on the conditions.. it can be a 200g mushroom, if there is no fresh air exchange or lack of humidity,or not enough nutritions in the substrate, it will never grow the same size.
-------------------- SPREAD THE SPORES
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: first flush small [Re: ballrawg]
#19195968 - 11/27/13 11:13 AM (10 years, 2 months ago) |
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Quote:
ballrawg said: I'm thinking it might be fae but i fan it 3 times a day. Should every single mushie be as big as my isolate? Wtf?
Fanning is not FAE. You would have to fan a few times an hour for FAE. In a monotub the small fan you have in the room+natural currents push fresh air into your monotub's top holes with loose polyfil that fresh air exits out the bottom tightly sutffed holes.
An isolate from clone tissue may perform poorly, if it wasn't an isolate and just left to be the clone culture you might have seen better results, It could also be old mycelium depending on how long or how many steps it took from spores to these fruits you're fruiting now.
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ballrawg
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Registered: 02/08/13
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Last seen: 8 years, 7 months
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So maybe I'm mistaken on the meaning of isolate. I just did a lc clone.
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Aero
Orea


Registered: 11/01/13
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Re: first flush small [Re: ballrawg]
#19196141 - 11/27/13 12:00 PM (10 years, 2 months ago) |
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Quote:
ballrawg said: So maybe I'm mistaken on the meaning of isolate. I just did a lc clone.
from the clone u can isolate a monoculture. seems like a better idea cause this way u can be pretty sure that your isolate is going to fruit
-------------------- SPREAD THE SPORES
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: first flush small [Re: Aero]
#19196301 - 11/27/13 12:41 PM (10 years, 2 months ago) |
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Quote:
Aero said:
Quote:
ballrawg said: So maybe I'm mistaken on the meaning of isolate. I just did a lc clone.
from the clone u can isolate a monoculture. seems like a better idea cause this way u can be pretty sure that your isolate is going to fruit
Not the best idea read through the thread I just posted. here. It goes into talking about clones/isolates/age of cultures/isolating from clones etc..
grade school level mycology: Clones are made up of many strains. A strain is two dykariotic mycelium IE two hyphae joining together. A hyphae is monokaryotic mycelium that's from a single spore germination. A clone is almost always made up of multiple strains so you would still have to isolate a single strain to have an isolate from a clone. However isolates from a clone MAY perform worse than the original fruit because the strains are not acting in chorus together, also you may see poor performance depending on how old the mycelium is IE spore>pf>fruit>clone tissue grows out in LC>LC inoc grains> grains inoc spawn> spawn fruits A better and more direct route would be spores to agar> agar to agar till you obtain an isolate > try out your isolate to see if its good. If you want to use clone tissue just keep it clone tissue and don't isolate anymore.
It could be your LC has a contam which is a likely possibility since most LC's made from clone tissue end up dirty.
It could be your clone fruit came from a PF cake and doesn't perform well on bulk
It could be too old of mycelium and it's not performing as well
It could be a bunch of things.
Edited by Trusted cuItivator (11/27/13 12:43 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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 don't isolate from a clone it's already proven it's fruiting ability
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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