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Periscope
eyeball


Registered: 08/16/13
Posts: 112
Last seen: 9 years, 9 months
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Syringe
#19191124 - 11/26/13 09:54 AM (10 years, 2 months ago) |
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Sorry for being a noob but this syringe is confusing me! i took off one piece of plastic and tried putting the needle on but there is a strange blue plastic thing in the way that has no holes in it or anything? What do I do? Please help
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 24 days, 11 hours
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Sounds like it's just the luer lock cap which is put on there to keep the syringe from leaking during transit. Twist the blue cap counterclockwise and it should come off. Then you can lock the needle in place of it.
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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you mean the syringe is capped off?
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underfliptown
I suck and you should kill me

Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 6 months
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I had this same problem. I think it screws in
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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I recently ordered from a vendor that i never used and the syringe came black cap that twisted off, then you open the needle and screw it on.. most vendors ive used mail em with the needle already on.. some dont
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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? just pull it off,might have to screw it off as said above..
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 24 days, 11 hours
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There are three primary types of disposable syringes. Luer lock, non locking, and irrigation tip. Forget about the irrigation tip, since that kind has a built in long tip which you can't affix anything additional to it. Those are what the dentist gives you to rinse your sockets with after you have a tooth pulled.
Non locking syringes are just what they sound like. Anything you attach (sharp or blunt needles, injection lines, etc.) just press fit into place. The end of the syringe will just have a nipple sticking out from it.
Luer lock syringes have the same nipple as a non locking syringe, but there's a second threaded enclosure around it allowing you to lock your needle/injection line, etc. in place.
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Periscope
eyeball


Registered: 08/16/13
Posts: 112
Last seen: 9 years, 9 months
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Thanks guys. I felt like every second I fought with it the more unsterile things got..hopefully all turns out well.would it be a bad idea to put the jars in the pot I boiled in? Waters drained but maybe a bit moist? Mold maybe?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
Periscope said: Thanks guys. I felt like every second I fought with it the more unsterile things got..hopefully all turns out well.would it be a bad idea to put the jars in the pot I boiled(steam sterilized) in? Waters drained but maybe a bit moist? Mold maybe?
You'll probably be fine just flame the tip before inoculation as usual and don't melt the plastic at the base of the needle.
How can you not completely dry out the pot you sterilized your jars in?
It's good for mushrooms/mycelium to get light during colonization
Quote:
Notahacker420 said: Lighting Requirements of Mushrooms
Some mushrooms, such as the Agaricus species commonly found in grocery stores require no light at all. However, those commonly grown by hobbyists, such as Pleurotus ostreatus (Oyster Mushrooms), Lentinus enodes (Shiitake), Psilocybe cubensis, a hallucinogenic mushroom, and Hericium erinaceus (Lion's Mane) all require light to produce abundant, normal sized fruits. Experience has taught us that the light best suited for primordia formation and the development of fruitbodies is bright light with a color temperature of 5,000 Kelvin to 7,000 Kelvin. Fortunately, this type of light is easily obtainable at your local home improvement center in the form of fluorescent fixtures. For a small terrarium as described in this chapter, a single CFL (compact fluorescent) that screws into a standard light bulb socket will work very well. These can often be found in grocery and drug stores in every neighborhood. 15 watt CFLs will do the job well, but the package will probably have a large 60 stamped on it, indicating they produce light "equivalent" to a 60 watt incandescent light bulb. They're referring to lumens of output, not the frequency. Incandescent light bulbs are the worst possible choice for growing mushrooms, since they emit a 'red' light in the 3,000 Kelvin color temperature range.
The higher the color temperature, expressed in Kelvin, the closer to the 'blue' end of the spectrum the emitted light is. The lower the color temperature the 'redder' the light is. If you have a choice of fluorescent lamps, purchase those labeled 'daylight' since these have a somewhat higher color temperature than cool white. Daylight, sometimes called 'natural daylight' fluorescent tubes generally emit light in the 6,500 Kelvin range, while cool white fluorescent emits light at around 5,000 Kelvin. If you have several terrariums stacked or otherwise near each other, you can use larger 2 to 4 tube fluorescent fixtures. These come in 48" and 96" lengths. Place the fluorescent lamps as close as you can get them to your terrariums without causing excessive heating. Species such as Shiitake and Oyster mushrooms prefer to fruit at temperatures in the upper 50's to mid 60's Fahrenheit (15C to 20C), while Psilocybe cubensis prefers to fruit at a temperature in the mid 70s to about 80 Fahrenheit (23C to 27C) Most mushroom species don't mind a slightly warmer temperature during daytime than at night, so if your grow room is a bit colder than the temperature ranges given above, a little warming from your lights during the daytime won't hurt at all, provided you don't let the air in your terrarium get too dry. For cakes, try to keep the humidity above 95%. Cased substrates are a bit more forgiving, but still try to keep your humidity above 90%. 12 hours on, 12 hours off has proved to be a great combination over a wide range of species. Of course, if you have a bright window near your terrarium, that will suffice, but direct sunlight for more than a few minutes per day should be avoided. Disregard outdated advice in old books which is constantly repeated on the internet to colonize mushroom substrates in total darkness. Experience and rigorous peer reviewed studies have proved that exposure to low level ambient indoor lighting during spawn run and substrate colonizing will speed up the process, leading to full colonization up to a few days earlier than the same substrate would if colonized in darkness. In addition, mushroom mycelium develops a day/night circadian rhythm, so exposure to light from day of inoculation sets this process in motion, leading to earlier fruiting and harvest.
Source: http://www.mushroomvideos.com/Terrarium-Tek
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Periscope
eyeball


Registered: 08/16/13
Posts: 112
Last seen: 9 years, 9 months
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Okay I put it in a desk I'll open it everyday. I didn't heat the needle too much. Hopefully it was enough. All I can do now is sit back and wait. Thanks guys
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