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Offlineotherr
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Registered: 07/01/13
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MY LC WORKED YES!
    #19188282 - 11/25/13 06:30 PM (10 years, 2 months ago)

AFTER MILLIONS OF TRIES IT FINALLY WORKED I WISH I COULD SHOW YOU GUYS PICTURES
AND SORRY FOR THE CAPS I'M JUST SO EXCITED THAT IT ACTUALLY WORKED!!!!!!
I know it's not important but I just wanted to share my excitement! For anyone that's curious I used 600 ML of water and two table spoons of honey!


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Offlinesilverstem
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Re: MY LC WORKED YES! [Re: otherr]
    #19188295 - 11/25/13 06:32 PM (10 years, 2 months ago)

congratz....


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Offlinefatflash
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Re: MY LC WORKED YES! [Re: otherr]
    #19188300 - 11/25/13 06:33 PM (10 years, 2 months ago)

Agar is superior.


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Offlinesilverstem
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Re: MY LC WORKED YES! [Re: fatflash]
    #19188305 - 11/25/13 06:34 PM (10 years, 2 months ago)

g2g is also superior.


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: otherr]
    #19188306 - 11/25/13 06:34 PM (10 years, 2 months ago)

Sounds like a lot of effort when you could have used agar and G2G :wink: JK, congrats. Now pray it doesn't contam when you stick the SHIP. Use a big syringe to cut down on the possibility of that occurring. 60 cc should do the trick.


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Offlineotherr
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188367 - 11/25/13 06:43 PM (10 years, 2 months ago)

Yeah before I posted this I thought to myself
"I should probably say that I know agar and g2g is easier"

It just seems like you can spread LC around the substrate a lot easier than agar


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: otherr]
    #19188390 - 11/25/13 06:47 PM (10 years, 2 months ago)

In the time it takes to colonize your LC, shoot up a test jar to see if its clean, then inoculate the rest of your spawn, you could have germinated your spores on agar, transfered once to be sure its clean, nocked up a master with a wedge, do your G2G and be done your spawn run :shrug:

LC is only useful for exotics IMO.


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Offlinesilverstem
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188396 - 11/25/13 06:47 PM (10 years, 2 months ago)

:whathesaid:


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Offlineunderfliptown
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Re: MY LC WORKED YES! [Re: otherr]
    #19188397 - 11/25/13 06:48 PM (10 years, 2 months ago)

lol ppl on here have such an annoying hard on over agar. It has actually made me never want to use it just to go against it.


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Offlinesilverstem
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Re: MY LC WORKED YES! [Re: underfliptown]
    #19188409 - 11/25/13 06:49 PM (10 years, 2 months ago)

Quote:

underfliptown said:
lol ppl on here have such an annoying hard on over agar. It has actually made me never want to use it just to go against it.



your lose... agar is awesome.


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: underfliptown]
    #19188412 - 11/25/13 06:49 PM (10 years, 2 months ago)

Quote:

underfliptown said:
lol ppl on here have such an annoying hard on over agar. It has actually made me never want to use it just to go against it.




Sorry but contams just make my penis go soft :lol:


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Offlineunderfliptown
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188416 - 11/25/13 06:50 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

underfliptown said:
lol ppl on here have such an annoying hard on over agar. It has actually made me never want to use it just to go against it.




Sorry but contams just make my penis go soft :lol:




hahahaha!

I mean yeah i will use agar when i go looking for it, but im ways away from that.


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: underfliptown]
    #19188443 - 11/25/13 06:53 PM (10 years, 2 months ago)

I started agar after my first and only pf tek. Cloned my first flush and never looked back.


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Offlineunderfliptown
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188465 - 11/25/13 06:58 PM (10 years, 2 months ago)

I was considering a tissue sample to grain transfer, but i guess the possibility of contams are pretty high. but the middle of a shroom stem is sterile so i don't see the point in agar.


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: underfliptown]
    #19188484 - 11/25/13 07:00 PM (10 years, 2 months ago)

Quote:

underfliptown said:
I was considering a tissue sample to grain transfer, but i guess the possibility of contams are pretty high. but the middle of a shroom stem is sterile so i don't see the point in agar.



:nojustno:

People have tried that, has about a 10% chance of working. Most of the time its just contam city. After you clone a lot you will learn that the inside of a shroom stem is not sterile.


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Offlineunderfliptown
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188509 - 11/25/13 07:04 PM (10 years, 2 months ago)

Have you heard about the tek where they use a syringe and alcohol. Then they wipe the outside of the shroom and stick the syringe in?

It seems pretty legit.


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InvisiblePastywhyteMDiscord
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Re: MY LC WORKED YES! [Re: underfliptown]
    #19188530 - 11/25/13 07:08 PM (10 years, 2 months ago)

I believe it was either creamcorn or the shroomy one who wrote that. Give it a go if you want but IMO its risky. Alcohol does not sterilize and if there is a mold spore on that fruit where you biopsy it, your LC is trash. Plus you probably won't know it until after you waste a lot of grain first. Seriously agar is not hard. If you can do pf tek, you can do agar. Besides the only LC that never contamed on me was inoculated with agar. Now I'm using LC for pans and p mexicana due to not being able to shake them, but there is no point in using it for cubes.


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Offlineotherr
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Re: MY LC WORKED YES! [Re: Pastywhyte]
    #19188593 - 11/25/13 07:21 PM (10 years, 2 months ago)

Yeah I've been planning to do agar, it just seems so annoying to do it in a SAB (But what isn't)

I just failed like 9 times with liquid culture and I really wanted to be successful with it!


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InvisibleSpitballJedi
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Re: MY LC WORKED YES! [Re: otherr]
    #19188801 - 11/25/13 07:57 PM (10 years, 2 months ago)

When you say "successful", do you mean you have fruits now? Or do you just mean you see growth in the liquid?


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