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Offlineunderfliptown
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Registered: 03/07/11
Posts: 14,344
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Can jars colonize from the inside out?
    #19188322 - 11/25/13 06:37 PM (10 years, 2 months ago)

I inoculated 10 jars on thursday (4daysago) and there is still zero sign of any colonization. Is it possible that they are colonizing from the middle out?


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Offlinekarp


Registered: 10/21/13
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188341 - 11/25/13 06:40 PM (10 years, 2 months ago)

4 days is still pretty early to see any colonization give it around 2 weeks and if you still don't see anything toss them.

as for the colonizing inside out it is possible, if you injected your spores right in the center of the cake.


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Offlinesilverstem
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Registered: 10/12/13
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188345 - 11/25/13 06:41 PM (10 years, 2 months ago)

4 days isnt enough time to see growth.


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Offlineunderfliptown
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Re: Can jars colonize from the inside out? [Re: silverstem]
    #19188358 - 11/25/13 06:42 PM (10 years, 2 months ago)

Well i shook them after the inoculation so it's possible that the spores are in the middle.


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Offlinesilverstem
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188392 - 11/25/13 06:47 PM (10 years, 2 months ago)

dont shake your jars...... unless its grains that are 30% colonized dont shake your jars... if its pf tek then you could cause the dry verm layer to change locations and allow contams to your cake.......


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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


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Offlineunderfliptown
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Re: Can jars colonize from the inside out? [Re: silverstem]
    #19188403 - 11/25/13 06:48 PM (10 years, 2 months ago)

It said in the tek i used to shake after noc for wbs.


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OfflineRubestoad
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188466 - 11/25/13 06:58 PM (10 years, 2 months ago)

id say use the search function.

your first post asking about wbs is in april of 2011 and you dont know this?


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Offlineunderfliptown
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Registered: 03/07/11
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Re: Can jars colonize from the inside out? [Re: Rubestoad]
    #19188476 - 11/25/13 06:59 PM (10 years, 2 months ago)

No i don't, thats why i asked :smile:

Back then I was doing pf tek. Haven't done anything since. I don't remember said post.


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OfflinePsilicon
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188540 - 11/25/13 07:09 PM (10 years, 2 months ago)

Yeah, I've definitely had WBS jars colonize from the inside out.  Two out of the last batch I did didn't look like they were colonized at all after three weeks, so I gave them a little shake to see if they were slimy.  Nope!  There was a raquetball-sized lump of mycelium right in the middle of both of them that for some reason wasn't touching the surface at all.  I shook them enough to break up the mycelium and redistribute, and they turned out just fine. :grin:


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Offlineunderfliptown
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Re: Can jars colonize from the inside out? [Re: Psilicon]
    #19188590 - 11/25/13 07:20 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Yeah, I've definitely had WBS jars colonize from the inside out.  Two out of the last batch I did didn't look like they were colonized at all after three weeks, so I gave them a little shake to see if they were slimy.  Nope!  There was a raquetball-sized lump of mycelium right in the middle of both of them that for some reason wasn't touching the surface at all.  I shook them enough to break up the mycelium and redistribute, and they turned out just fine. :grin:




ahhhh nice!

That is really nice to hear. I am getting nervous here. Patience is so hard in this endeavor.


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OfflinePsilicon
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Re: Can jars colonize from the inside out? [Re: underfliptown]
    #19188603 - 11/25/13 07:22 PM (10 years, 2 months ago)

I would consider beginning to get nervous if you see no growth ten days from now.  While I've had growth within four days of inoculation before, I considered it a one-off and a minor miracle.

Patience only applies if you don't throw yourself at the hobby head-first like so many of us here have.  Stop looking at your jars and start looking for a place to put your next dozen.  Tomorrow's a good time to cook, right?  :wink:


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OfflineOgreLokon
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Re: Can jars colonize from the inside out? [Re: karp]
    #19188614 - 11/25/13 07:24 PM (10 years, 2 months ago)

Quote:

karp said:
4 days is still pretty early to see any colonization give it around 2 weeks and if you still don't see anything toss them.




I'd say to give them even longer... Hell, don't throw them out until you see contams, or need the jars. :2cents:


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OfflinePsilicon
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Re: Can jars colonize from the inside out? [Re: OgreLokon]
    #19188617 - 11/25/13 07:25 PM (10 years, 2 months ago)

Quote:

OgreLokon said:
Quote:

karp said:
4 days is still pretty early to see any colonization give it around 2 weeks and if you still don't see anything toss them.




I'd say to give them even longer... Hell, don't throw them out until you see contams, or need the jars. :2cents:




:whathesaid:

I couldn't agree more.


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Offlineunderfliptown
I suck and you should kill me

Registered: 03/07/11
Posts: 14,344
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Re: Can jars colonize from the inside out? [Re: Psilicon]
    #19188643 - 11/25/13 07:29 PM (10 years, 2 months ago)

I have another syringe of R44, but i really wanted to get some zstrain going first so that I could do some g2g and then save the r44 for when my tub is going.

I need more jars...


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