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OfflineMosey3012
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Registered: 12/12/12
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Last seen: 3 months, 15 days
Agar Masters - Teach Me Cutz - PICS*
    #19187532 - 11/25/13 03:22 PM (10 years, 2 months ago)

First thing first, although I've been having success growing out and making clean xfer's with agar for about a year now onto grains or more agar/what ever the case may be I've yet to get any sort of isolation going from what I can tell (although I've been trying).

I'll be using this plate as an example because not only did the pics come up more clear then normal but the dark agar allows for better clarity and its the most recent clone sample I've taken from a still ongoing grow. So a few questions using this pic as well as suggestions on where to take cuttings from is what im looking at here... Feel free to skip the questions and just mention where and y you'd cut a particular place over another if you'd like.

Something I'd like to note and get a little feedback on if possible...

A- Notice how fluffy that has grown out? Is this normal for clones tissue to grow out that fluffy?
I ask this because I feel like I've seen people with pics of clones or even spore germination on agar that has had obviously ropey sections visible right off the bat...
Based on my limited knowledge on the matter... I can only assume there's no very visible ropeyness from any rizo growth because there's so many different sets of genetics growing out so close to each other?
All of my plates seem to grow out this way. Now in these pics (surprisingly) you're able to see on the outter edge of the growing ring some sectoring occurring. I marked off what to my knowledge in the green would more then likely be tomentose growth then in blue where I'd likely get more ropeyness if making a transfer from there?
*Zoom in pic to see markings* (We can call left green sector A, Blue: B, and to the right green sector C)



The original


Out Some


I've been attempting to take small sections from the outter edge of my plates from one to the next for at least 5 or 6 plates of the same culture and it still seems to grow out in that similar fuzzyness... doesnt ever seem to grow out with more defined sections ever...

So if this were you where would you attempt to make your cutting from on this plate coming off a fresh cloned AA+?

What might i be doing wrong here to cause a constantly fuzzy growth limbo?!


--------------------

"Each of these lives is the right one! Every path is the right path. Everything could have been anything else and it would have just as much meaning"



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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Re: Agar Masters - Teach Me Cutz - PICS* [Re: Mosey3012]
    #19187648 - 11/25/13 03:55 PM (10 years, 2 months ago)

first off what's your agar recipe? That stuff is really dark, hpoo water? Most of the time I start with clone tissue the myc runs out rhizo right away but I make my agar weak something like 500ml h2o 9-10g agar and 9-10g malt extract powder(I cut back on this to 8.5-9 usually)


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Offlinebakenast
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Registered: 03/14/11
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Re: Agar Masters - Teach Me Cutz - PICS* [Re: bodhisatta]
    #19188185 - 11/25/13 06:09 PM (10 years, 2 months ago)

mine normaly start out puffy, just at the inoculation before flying across the agar


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Agar Masters - Teach Me Cutz - PICS* [Re: bakenast]
    #19188277 - 11/25/13 06:30 PM (10 years, 2 months ago)

Could be genetic,  agar could be too rich, could be a contam along for the ride. You won't know until ya do a transfer or two.


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