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Offlinekrypto2000
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Fuzzy looking hpoo 24 hours after spawning?
    #19186989 - 11/25/13 01:18 PM (10 years, 2 months ago)

Not sure if this is normal or not, I don't tend to inspect things very thoroughly until I'm ready to initiate fruiting, but I happened to notice in the corner of this little mini tub that there is a piece of poo which is fuzzy already, it can't be more than 30hrs after spawning. It looks like it might be cobweb, but it might be mycilium that hasn't filled in as well. The spawn hasn't even fully recoered yet it has happened so fast, as in it's growing, but pieces of the grains are still visible at this point.

After noticing it I looked in there with a flashlight and saw a few more pieces of poo here and there with what looks like wispy fuzz on it. Is it possible some loose myc just started colonizing the poo? I thought cobweb due to the wispyness of it and how fast it appeared, but even if it were cobweb or really any contam I'd typically expect it to start from a grain and transfer to the substrate, but it looks like it grew strait out of the poo which is why it's so odd. Jars looked and smelled good (I actually forgot to do a sniff test, but I could smell it in the air when spawning). The poo was pasturized in jars on the oven, kept at 150f +/- a few degrees for about 90 min, put a lid on jars (which were covered in foil already) and stored em inthe grow room until the next morning, which would be yesterday. I'll know one way or the other soon enough since cobweb grows so fast, but just wanted to get some thoughts, I could see this being normal and I just never noticed before. Since this little tub is so tiny and the walls are clear (and not lined with anything) I can get a better view than in a mono which is my normal fruiting method.


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OfflineSurreal
Tough puffin' wizard


Registered: 11/23/13
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19187035 - 11/25/13 01:30 PM (10 years, 2 months ago)

It sounds like cobweb from incomplete pasteurization. Sometimes hydrogen peroxide on the affected area will subdue it and you will still get fruits, but if its only been 24 hours it sounds like its history already.


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: Surreal]
    #19187046 - 11/25/13 01:33 PM (10 years, 2 months ago)

I would think it'd of had to come from the spawn if anything. I wouldn't expect even strait coffee to contaminate after only a day. I've left wet coffee grains in my pot before and even just sitting out like that and being unpasturized (though perhaps partially sterilized from the drip) you won't see growth until after a few days.


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InvisiblebodhisattaMDiscordReddit
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19187109 - 11/25/13 01:50 PM (10 years, 2 months ago)

You can put a whole turkey in a 350F oven for 5 hours and the inside will just barely be getting to 160. When you pasteurize a jar in the oven at 150 it's going to take a hell of a lot longer than 90M for it to come up to temperature. Besides that the core has to be 140-160F for a full hour. If your jars started out at room temp and you put them in the oven that was set at 150F it would take hours alone for it to even get to pasteurization temperatures. You need to use a water bath next time.


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: bodhisatta]
    #19187143 - 11/25/13 01:57 PM (10 years, 2 months ago)

My fault, I meant stove, not oven. I followed RR's tek. It was a small tub so I only used 2 jars. One jar I put a lid on, the other I put foil on and stuck a probe thermometer in it. I also stuck a thermocoupler in the pot of water. I didn't start the clock until the probe read 140f and the water was kept at 160f (highest point was probably no more than ~163), and after the probe reached 150f I even cut the heat back a bit and dropped the water to ~150f, so it definitely held the temp fine and there was no way it got too hot either since the water wouldn't allow it. I'd still be surprised if even non pasturized poo grew mold in 24hr unless it already had some visibly growing on it. That does raise the question though, if the poo did have mold growing on it (sometimes poo will have white fuzz on it after all) I don't imagine the mold would totally dissappear just from pasturizing it would it? Not saying it's still viable, but it still in the substrate, could I just be seeing some dead mold?


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InvisibleJosh.0
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19187277 - 11/25/13 02:24 PM (10 years, 2 months ago)

:shrug:Can we see?


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: Josh.0]
    #19187390 - 11/25/13 02:47 PM (10 years, 2 months ago)

I'll see if I can get a camera. It's very clear atm with the naked eye, hopefully I can get a good pic of it.

edit: If cobweb came from my spawn wouldn't you expect it to show up, especially after shaking it? I don't think I've ever had cobweb on a bulk substrate before (at least not without forgetting it and letting it sit way too long), but if it's ever showed up in a jar normally the whole things overtaken in a matter of days.


Edited by krypto2000 (11/25/13 02:50 PM)


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InvisibleJosh.0
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19187456 - 11/25/13 03:03 PM (10 years, 2 months ago)

Quote:

krypto2000 said:
if it's ever showed up in a jar normally the whole things overtaken in a matter of days.




unfortunately the same is true with a whole monotub :sad:
sending shroomy vibes your way!:shroomeryhead:


--------------------
:trippinbawelz:                              :trippinballs::mindblown::trippinballs:                      :awecid:

            :mushdance:                              :growshrooms:                                    :mushdance:


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: Josh.0]
    #19187550 - 11/25/13 03:27 PM (10 years, 2 months ago)

Yeah, that's why I'm saying I'll find out soon enough one way or another, but thanks for the thoughts : ). Just wondering what I did wrong if it is cobweb though, I would assume it'd have to of been hidden in the spawn, but cobweb seems hard to hide.

I did get a camera so should have some pics up soon, not sure if they'll turn out though. I also just noticed on another spot which is doing the same thing (there are quite a lot of spots like this) that this one is actually much denser and whiter which does look like mycilium, cobweb never gets thick enough to turn white and looks more gray anyway, I think due to sporulation. Either way this is a bit confusing to me, if it's myc then it is growing (as it appears on the macroscopic level anyway) faster on the poo than it is on the grains, the grains aren't even fuzzy yet. Generally I'd expect a bit of a delay while the myc moves from the grain to the poo, maybe it's just fast, or even just that I've never actually looked that closely before. Anyway should have some pics up within the hour.


Edited by krypto2000 (11/25/13 03:28 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19187584 - 11/25/13 03:38 PM (10 years, 2 months ago)

Hard to say without the pics but I would definitely say it's your spawn then. Did you inoc your grain jars with MS syringe, LC, agar, or g2g?


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: bodhisatta]
    #19187663 - 11/25/13 03:57 PM (10 years, 2 months ago)

It was from a previously tested LC. I noced up 2 test jars (these might even be them?), waited a week or so to make sure I only saw healthy myc growth and then noced up some more. Lost a set of jars to bacteria, but I blame that on there being to much free starches in the grain due to it having a bunch of corn in it (different brand than I normally use). These are the first ones I've spawned though so hopefully there's not hidden cobweb in all of these :mad:.

Here's the two best pics I was able to take. They're clear enough that you can see something is growing on the poo, but not sure if they're clear enough to tell what that something is. I'll try to take some more as it grows if anyone is curious at that point.



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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19204241 - 11/29/13 10:00 AM (10 years, 2 months ago)

Well just to update it is def not cobweb, and it doesn't seem to be spreading or causing a problem. The myc's just about fully covered it up now, or maybe it was myc and it's just... yunno.. that explains it lol. Probably about 2 or 3 more days and it'll be fully colonized


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OfflineSurreal
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19231427 - 12/05/13 02:10 PM (10 years, 2 months ago)

Happy to hear that the tub is going well. Usually I put a layer of substrate on top of my grain spawn so if I see anything within 24 hours its not mycelium, because it takes longer than that to break the suface. Happy fruiting.


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Fuzzy looking hpoo 24 hours after spawning? [Re: Surreal]
    #19231583 - 12/05/13 02:49 PM (10 years, 2 months ago)

This spring I tossed out a whole 5 gallon bucket full of colonizing yellow oyster because I thought it was cobweb.  Im glad to hear that your grow is on track for success.


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: HypnotoadCroaked]
    #19232134 - 12/05/13 04:45 PM (10 years, 2 months ago)

Well the bin just showed some trich yesterday so all is not so great. It's a clear tub with no liner and I noticed a tiny spec of green pressed against the wall. Actually that was two days ago, yesterday I noticed a spec on the top. I tried to pluck it off with a knife, but I could no longer find it when I went to do it. Spotted it today and got it though. I've had success in the past doing that if it's really small and the sub is already consolidated. The one on the side doesn't seem to be growing at all.

Pretty sure this has nothing to do with that fuzz though, it definitely wasn't trich and seemed to grow into the mush, which is strange because that is some super fast mycilium if that's actually what it was. No pins yet, but can't be more than a few days away, it's starting to knot up already. I'd give this a 50/50 chance of getting a flush out of it after which I'll toss it regardless. Not too worried about it though, I've got 2 more regular monos colonizing and enough jars nearly done to make a few more.


Edited by krypto2000 (12/05/13 04:46 PM)


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InvisibleHypnotoadCroaked
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: krypto2000]
    #19232232 - 12/05/13 05:09 PM (10 years, 2 months ago)

Quote:

krypto2000 said:
Well the bin just showed some trich yesterday



Ohgoddamn.  I hate to hear that.  I hate that shit.

As odd as it sounds I still have yet to see cobweb.  I have been cultivating differing species of mushrooms for almost 2 years now, and as far as I can tell, nothing has cobwebbed.  However I have seen my fair share of the green.  I have only had one incident where I extracted the infection and continued along....it also happened to be my first cube grow lol.  Its like the higher power wanted me to succeed once so that I could get the taste, and want more.

Every time I got the tric, I did what you seem to plan.  Run it out till the end of the flush if possible, burn it with fire, and bury it in an unmarked grave next to all the cat skeletons.


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Offlinekrypto2000
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: HypnotoadCroaked]
    #19232899 - 12/05/13 05:24 PM (10 years, 2 months ago)

Yeah, cobweb is pretty rare ime. The only time I've seen it were by leaving an unsterilized grain jar somewhere for too long or once when I left a spent bin sitting for *way* too long.


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Offlinemastercultivator
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Re: Fuzzy looking hpoo 24 hours after spawning? [Re: Surreal]
    #19232967 - 12/05/13 05:36 PM (10 years, 2 months ago)

This is my response.  The first response was the best.

you are shooting for 170 for pasturization.  once the center hits 170 there is usually a thermal runaway to 190 and it holds at 170 on the cool down for at least 1 hour so you are good.  The good bacteria take hold and everything is good. H2O2 mix spray helps a lot too with any contam issues that might come up.  10ml of 3% store bought per liter of water is a good spray concentration to use.

Ya live and learn. 

Quote:

Surreal said:
It sounds like cobweb from incomplete pasteurization. Sometimes hydrogen peroxide on the affected area will subdue it and you will still get fruits, but if its only been 24 hours it sounds like its history already.




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