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Offlinepiffle8
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Registered: 11/04/13
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Last seen: 6 years, 4 months
Oyster-Cardboard-Clone Problem
    #19187006 - 11/25/13 01:22 PM (10 years, 2 months ago)

Have grown from fugi-perfecti kits so I know the bare basics of pasteurizing straw and keeping humidity good and inducing fruiting conditions, etc...But I haven't advanced to going from spore to agar and injecting jars of WBS and PCing and all that, my intermediate step is cloning (if that's the correct term) stems from the mushrooms from the kit grows.

The problem I keep getting is that the first jump from stem pieces to corrugated cardboard layered in a ZipLock back starts out great. Within a few days everything's white and fuzzy like crazy. Si I try to jump it up again to more cardboard by un-peeling the original layers and re-layering each piece between new cardboard.

Alternatively I've attempted to break up the white fuzzy cardboard and put in pasteurized straw and/or coffee grounds in a Zip-Lock or one gallon ice cream bucket or whatever, and everything just stalls. After a week or so I'll look, and there is no contams that I can see or smell, I believe the moisture, humidity, temperature, fresh air, etc is the exact same as when I started with just stems and cardboard, but the crazy growth from before stops.

I've tried searching online for answers as well as simply replicating the attempt with slight changes to a variable, etc...to no avail. The initial jump from oyster stem slices to cardboard rocks like a hurricane, then the attempt to continue to run the mycelium on larger amounts of cardboard/straw just stall out.

I know Stamets says "move it or lose it," is it possible that I am waiting too long to transfer it and it's just done? Out of ideas of what to do differently, and would love some advice.


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InvisibleMObeek
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Re: Oyster-Cardboard-Clone Problem [Re: piffle8]
    #19188930 - 11/25/13 08:32 PM (10 years, 2 months ago)

Since I'm a newbie, I don't know the answer to your question.  However, I'm sort of copying your method of jumping mycelium growth on straw.  I hope yours eventually continue to grow again.  Please keep us updated on your mycelium's progress.


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Offlinepiffle8
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Re: Oyster-Cardboard-Clone Problem [Re: MObeek]
    #19190698 - 11/26/13 07:25 AM (10 years, 2 months ago)

So you just put stem pieces on straw instead of cardboard? How does that work for you?


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OfflineRogerRabbitM
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Re: Oyster-Cardboard-Clone Problem [Re: piffle8]
    #19190940 - 11/26/13 08:59 AM (10 years, 2 months ago)

The cardboard thing really only works well when you're going to immediately inoculate an outdoor patch of sawdust/chips.  I suspect the cardboard is growing some bacteria which is inhibiting the mycelium, or perhaps it doesn't like the lime or other composition of the cardboard.

I'd recommend learning to do agar work.  It sounds like you're getting hooked on the hobby so it's time to get a flowhood, etc. :wink:
RR


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Offlinepiffle8
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Registered: 11/04/13
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Re: Oyster-Cardboard-Clone Problem [Re: RogerRabbit]
    #19193437 - 11/26/13 06:19 PM (10 years, 2 months ago)

Quote:

RogerRabbit said:


I'd recommend learning to do agar work.  It sounds like you're getting hooked on the hobby so it's time to get a flowhood, etc. :wink:
RR




Thanks, yeah, I'm absolutely getting hooked on it, and my kids are learning along with me. I tend to jump into things and ramp up too fast so I thought I'd take mushrooms slower and progress from kits and cloning all the way up to collecting spore prints and inoculating grain and all that stuff...


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Offlinethiotimoline
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Re: Oyster-Cardboard-Clone Problem [Re: piffle8]
    #19193645 - 11/26/13 07:00 PM (10 years, 2 months ago)

A still air box will be fine for your agar work until you're working on a much larger scale. The things you should actually buy at this point are
  • Pressure cooker
  • Agar (available at Asian groceries)
  • Malt extract (available at brewing supply shops) or some other nutrient source
  • Plastic bin for still air box
  • Petri dishes, short half-pint jars, or some other sterile or sterilizable container to pour your agar into


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Offlinepiffle8
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Re: Oyster-Cardboard-Clone Problem [Re: thiotimoline]
    #19194155 - 11/26/13 08:48 PM (10 years, 2 months ago)

Cool, thanks for the reply.......


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OfflineForresterM
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Re: Oyster-Cardboard-Clone Problem [Re: piffle8]
    #19194474 - 11/26/13 10:17 PM (10 years, 2 months ago)

What RR said!

But if agar work seems a little daunting, as it did for me at first, maybe just get a PC and buy an LC syringe from a vendor.  Make your own grain spawn, inoculate with LC, and you don't need a still air box, or to mess with agar. 

It's kind of a good "mid-way" step to going full out with agar and all that...


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinepiffle8
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Registered: 11/04/13
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Re: Oyster-Cardboard-Clone Problem [Re: Forrester]
    #19197304 - 11/27/13 04:45 PM (10 years, 2 months ago)

Absolutely, that's exactly what I was thinking.......Thanks again.......


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