Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
How can this syringe be saved?
    #19185572 - 11/25/13 06:23 AM (10 years, 2 months ago)

There is a spore syringe that was purchased that repeatedly produces what looks like a strange contam when using on agar. Within a day, the areas where the water has touched/rolled on the agar will turn a bright pinkish red almost resembling dilute blood, then it seems to gelatinize(is that a word?) and take on a beige color. After that it seems to stop progressing.
Every plate made with the syringe turns out this way no matter how sterile the technique making me think it's a problem with the syringe itself.
So, wanting to be a proper agartista, how would someone go about growing anything and then separating in this environment?
There are several plates new plates with this now and there are visible spores present. Whether or not the spores will germinate in the first place is the big question since they all are in this gook. What to do if they manage to grow is another.

Perhaps a hot agar bath? But do the spores need to germinate first?
Or maybe a antibiotic agar will need to be made?
Maybe there is a way to remove this pinking fluid before it gets a foothold atop the spores and agar?


--------------------


Extras: Filter Print Post Top
OfflineSamhainJ
I wanna rock out, in my dreams
Registered: 05/16/04
Posts: 1,002
Loc: USA Flag
Last seen: 8 years, 7 months
Re: How can this syringe be saved? [Re: NOS4A2]
    #19185595 - 11/25/13 06:35 AM (10 years, 2 months ago)

Wouldnt it just be worth it to toss the spike if its contamed aand make or buy a new one?


Extras: Filter Print Post Top
OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,504
Loc: USA Flag
Last seen: 1 hour, 2 minutes
Re: How can this syringe be saved? [Re: SamhainJ]
    #19185648 - 11/25/13 07:03 AM (10 years, 2 months ago)

I'm still a noob so this may not be the best way to go about it, but what I would do is inoculated a grain jar and hope that some decent mycelium forms out of it. Then, grain to agar and try to isolate from contamination. What type of mushroom is it btw?


Extras: Filter Print Post Top
InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: How can this syringe be saved? [Re: SupaThaRipper]
    #19186225 - 11/25/13 10:18 AM (10 years, 2 months ago)

You may have a good idea Supa. Maybe if the water were allowed to drain away from the spores they'd have a better chance.
I was told it was B+. The first time it was opened it got stuck into the nail bed of a finger accidentally, so I don't want to discredit this well known vendor for sending a bad sample. However, a different vendor will be chosen next time. The less that has to be ordered the safer, so if something can at least be gained from this syringe the better.

Not to mention as stated by more experienced cultivators before, a good cultivator would be able to transfer the contam away. I like the grain idea which might be tried but agar is made for this sort of situation.
Maybe if I can get a pic of the sample it will spark some ideas...


--------------------


Extras: Filter Print Post Top
InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: How can this syringe be saved? [Re: NOS4A2]
    #19186366 - 11/25/13 10:49 AM (10 years, 2 months ago)

Can a syringe be PC'd to a certain temp killing bacteria but sparing the spores?

Or perhaps the contents could be removed, treated, quickly dried out in a shot glass, then rehydrated with a peroxidated solution?


--------------------


Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
Re: How can this syringe be saved? [Re: NOS4A2]
    #19189306 - 11/25/13 10:03 PM (10 years, 2 months ago)

What you *can* do is sterilize a shot glass full of water, shake the syringe thoroughly and inject a single drop of spore solution into the glass.  Swirl to mix.  Then, with a loop or other inoculating device, remove a tiny quantity of that water and streak it in a zigzag pattern across your agar.

What you *should* do is call up the provider of the syringe and tell him that under a microscope it appears the syringe is contaminated with some sort of bacteria, and that it's unsuitable for microscopy.


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 39 minutes
Trusted Cultivator
Re: How can this syringe be saved? [Re: NOS4A2]
    #19189554 - 11/25/13 10:47 PM (10 years, 2 months ago)

You could try antibiotic agar. Don't know how much it would help though if it's that heavily contaminated. Be sure you're only a tiny drop to incoulate the agar. It's easy to over do it and no mycelium if there's too much moisture.


--------------------


Extras: Filter Print Post Top
InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: How can this syringe be saved? [Re: Kizzle]
    #19191142 - 11/26/13 10:00 AM (10 years, 2 months ago)

'What you *can* do is sterilize a shot glass full of water, shake the syringe thoroughly and inject a single drop of spore solution into the glass.  Swirl to mix.  Then, with a loop or other inoculating device, remove a tiny quantity of that water and streak it in a zigzag pattern across your agar.'

good one

'What you *should* do is call up the provider of the syringe and tell him that under a microscope it appears the syringe is contaminated with some sort of bacteria, and that it's unsuitable for microscopy.'

like I said after sticking it into a finger before even the first innoc with that syringe, it can't be honestly argued that it was contammed before that. Saving a buck just isn't that important in this case. Lesson learned.

'You could try antibiotic agar. Don't know how much it would help though if it's that heavily contaminated. Be sure you're only a tiny drop to incoulate the agar. It's easy to over do it and no mycelium if there's too much moisture.  '

I guess this is as good a time as any to learn to make antibiotic agar. And yes, scraping some spores onto agar is a lot cleaner process than dripping them from a syringe. I really don't think using a syringe for agar will happen in the future...

I was kinda hoping someone like FrankHorrigan with a considerable more experience woulda chimed in by now. Especially when he's chastising people for not working with contammed cultures and just tossing them.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* how do you tell if a syring is contaminated HarvestTheBrain 2,394 3 07/17/03 01:08 PM
by MAIA
* antibiotic spray? comario2 1,557 11 01/13/03 11:19 AM
by Mycosaurus
* Hoping for some help to save my GT's soon from pinhead mold scatmanrav 2,007 6 08/11/04 06:02 PM
by scatmanrav
* Can contaminated spore water be saved? mesq 2,741 5 03/15/02 01:24 PM
by rungi
* H202 and Antibiotics SeatleJ 875 5 01/02/06 08:51 PM
by RogerRabbit
* Amoxicillin(antibiotic) and Shrooms BathTearSun 9,440 4 03/22/05 01:40 PM
by BathTearSun
* Areosol antibacterial, or antibiotic added to casing layer. *DELETED* OOKLA 848 2 04/24/03 07:38 PM
by Seuss
* How can I save this sweet, sweet TENN POO..Help ... PICS! ebass 1,951 19 05/21/06 07:14 PM
by kristen

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
695 topic views. 38 members, 209 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 14 queries.