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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: lab sterilization [Re: PussyFart]
#19161309 - 11/19/13 07:02 PM (10 years, 3 months ago) |
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Your dead skin cells should never be able to fall in your work if your sterile technique is up to par. Your hands should never be over top of anything sterile for any reason.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: lab sterilization [Re: 36fuckin5]
#19161371 - 11/19/13 07:10 PM (10 years, 3 months ago) |
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Quote:
36fuckin5 said: Your dead skin cells should never be able to fall in your work if your sterile technique is up to par. Your hands should never be over top of anything sterile for any reason.
But still, the fact that my hands are always moving around in there.....bare skin just does not sit well with me.
6 months ago I felt the same way about it you do.....then frank made a good point about the shedding of cells and it makes sense.
Better safe than sorry regardless.....this is why I have a set of tyvek sleeves attached to my SAB and I wear a pair, with gloves.....lol...OCD redundancy at it's best.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Re: lab sterilization [Re: 36fuckin5]
#19161535 - 11/19/13 07:38 PM (10 years, 3 months ago) |
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Skin cells don't just shed and fall straight down. They enter the air currents and can go everywhere. Always wear surgical gloves and either tyvek sleeves or a freshly laundered long-sleeve shirt.
A decade or so ago when lots of folks decided it would be a good idea to shower and then work naked, contamination rates skyrocketed. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 1 month
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Re: lab sterilization [Re: PussyFart]
#19161721 - 11/19/13 08:13 PM (10 years, 3 months ago) |
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A friend who had worked in a sterile lab used a protocol calling for 2 layers of gloves. I just started doing this, and I love it. I've always been concerned about my nitrile surgical gloves slipping off an end of the Tyvek arm sleeve, exposing a patch of hairy wrist. With this technique, there are no worries. Here's the procedure.
1. Put on one set of gloves. 2. Put on arm sleeves, with the elastic sleeve ends overlapping the tops of the gloves. 3. Put on the second set of gloves, with the glove tops overlapping the ends of the elastic sleeves.
-------------------- Liberté, égalité, humidité.
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 11 months
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Quote:
RogerRabbit said:
A decade or so ago when lots of folks decided it would be a good idea to shower and then work naked, contamination rates skyrocketed. RR
hahaha that's hilarious!
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Kalypto
Psychonaut



Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
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Re: lab sterilization [Re: drake89]
#19169736 - 11/21/13 12:09 PM (10 years, 3 months ago) |
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Haha thats great
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monoculture
Mycaddict



Registered: 10/16/13
Posts: 246
Last seen: 9 years, 6 months
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Re: lab sterilization [Re: Kalypto]
#19174524 - 11/22/13 11:04 AM (10 years, 3 months ago) |
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I do like the square hole in that box though. That is much safer to uncap a syringe than the two round holes are. I found out the hard way, I already knew the right way to uncap a syringe needle is to put both wrists together and pull the cap off that way so there is no chance of stinging yourself with the needle. With the 2 round hole design, there is a very big chance of stinging yourself, because you cannot get the wrists together and when pulling the cap off, your wrists or arms will hit the outside of the round holes, triggering the reflex to put your hands back together.
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cArcace-x
YeAh!!


Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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hold it tight and push your finger together, it will open smoothly
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djoggy
Stranger
Registered: 11/21/13
Posts: 18
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Re: lab sterilization [Re: cArcace-x]
#19177896 - 11/23/13 07:24 AM (10 years, 3 months ago) |
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What dude, just go to fin wallmart and get like 20 cans of lysol. Spray it in the room until you are breathing lysol and then leave the room for like an hour. Then enter the room, spray some more, and cover yours arms and face with 70 % rubbing alcohol and don't breath on the area.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 4 hours
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Re: lab sterilization [Re: djoggy]
#19184720 - 11/24/13 10:07 PM (10 years, 2 months ago) |
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Quote:
so guys... don't spray alcohol everywhere if you are going to flame sterilize... or just wait a little bit until it dries up.
Yeah wait for it evaporate because alcohol vapor isn't flammable right? 
Quote:
In my opinion a tool sterilized outside will have to travel through the outside atmosphere however short as your hands push air inside as well which can contaminate your still air environment just the same as an ill aimed butane pencil torch inside a large GB that never opens and has been redundantly sterilized and let to settle.
You would think so but that doesn't seem the be the case. I can't even explain why I can only assume the air is still enough that any incoming particles settle quickly. Although from science based perspective I don't see how new particles pushed in could settle that quickly, they would probably still have a lot of momentum. I would think it would a couple minutes for them to settle even in still air. That's the problem, trying to figure out the exact behavior of thing that you can't even see. The box works though I guess that's what really matters.
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Terry M
Stranger in a Strange Land



Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
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Re: lab sterilization [Re: Kizzle]
#19185501 - 11/25/13 05:27 AM (10 years, 2 months ago) |
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I think that if you move your hands slowing and deliberately, a minimum of outside air gets into the SAB. Of course, slow and deliberate hand movement is proper sterile practice for any environment. Have always used an external alcohol burner with a SAB/GB and have never had a problems. Everything you need should be in the box before you start, of course, before the settling period.
-------------------- Liberté, égalité, humidité.
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NOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: lab sterilization [Re: Terry M]
#19185524 - 11/25/13 05:46 AM (10 years, 2 months ago) |
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I think that is where I make the most mistakes; trying to reach for something in my GB but my arms are forced to stay in one spot and limited by the length of the gloves. Did the whole self-stabbing thing with a syringe too. Man that sucks. Automatically contamed the whole syringe too. I don't think I ever had a problem uncapping with my own needles before. Definitely a learning experience there lol.
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leschampignons
Biochemistry + Mycology


Registered: 08/30/13
Posts: 1,583
Loc: NY/NJ/ME
Last seen: 28 days, 6 hours
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Re: lab sterilization [Re: Kizzle]
#19187763 - 11/25/13 04:28 PM (10 years, 2 months ago) |
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Quote:
Kizzle said:That's the problem, trying to figure out the exact behavior of thing that you can't even see. The box works though I guess that's what really matters.
Maybe you could see the currents if you lit it up with high powered lighting. Ever have the sun shine in through the window and illuminate all the dust in the air? And then you're like damn look at all this shit I breathe in all the time lol. This might be a worthwhile experiment to perform to see how still my SAB is...You could also see how long it takes to settle if you set it up right.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: lab sterilization [Re: Terry M]
#19191766 - 11/26/13 12:24 PM (10 years, 2 months ago) |
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Quote:
Terry M said: Of course, slow and deliberate hand movement is proper sterile practice for any environment.
Not true. You only ever want to be slow in a SAB. Any other time you want to move as quickly as you can while still being very precise.
If you want to see how air currents in your SAB work, clean it with soap & water then put a lit stick of incense in there. Watch how it flows.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 4 hours
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Quote:
leschampignons said:
Quote:
Kizzle said:That's the problem, trying to figure out the exact behavior of thing that you can't even see. The box works though I guess that's what really matters.
Maybe you could see the currents if you lit it up with high powered lighting. Ever have the sun shine in through the window and illuminate all the dust in the air? And then you're like damn look at all this shit I breathe in all the time lol. This might be a worthwhile experiment to perform to see how still my SAB is...You could also see how long it takes to settle if you set it up right.
That might give you a good approximation of things like bacteria that are usually clinging to larger particles. Some mold spores are very good at staying airborne, possibly to the point that they're effectively removed in the box during the spray down.
One thing I am certain of is once they do settle they don't become airborne again easily so stirring them up from minor air currents created from moving your arms around isn't really an issue. You can think of it like a tornado blowing over a hole. On the scale of mold spores even a smooth surface is rugged so those currents don't reach them easily.
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solumvita
Q.B.E.


Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 8 months, 12 days
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Re: lab sterilization [Re: Kizzle]
#19241188 - 12/07/13 02:24 PM (10 years, 2 months ago) |
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a flame inside the box is fine, a glove box or a still air box makes no difference essentially you are looking at restricting air currents and being able to work aseptically.
it is the technique that makes most of the difference, if you can keep contamination to less than 3% in a box you are in the 'accepted' range and less than 0.1% in a laminar flow hood.
all the rest is academic thereafter
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: lab sterilization [Re: solumvita]
#19241259 - 12/07/13 02:36 PM (10 years, 2 months ago) |
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Quote:
solumvita said: a flame inside the box is fine, a glove box or a still air box makes no difference essentially you are looking at restricting air currents and being able to work aseptically.
A flame inside a SAB or a GB will create heat currents whish will defeat the purpose of a "still air environment".
Also flaming inside the box is a terrible idea if you use anything flammable such as lysol or alcohol inside the box....
And a SAB where the gloves are attached to you rather than a GB where the gloves are attached to the box is actually better.
When the gloves are attached to the box, every time you move your arms around air will get pushed in and out of the box thru the imperfect seal on the lid....again defeating the whole purpose of having a "still air environment".
Having gloves attached to the box rather than your hands also makes getting your hands in and out of the gloves a pain and getting your gloved hands in and out of the box impossible, which is not good when flame sterilizing your tools outside the box.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Ultron
alchemist programmer




Registered: 08/04/13
Posts: 751
Loc: inner sanctum of the cosm...
Last seen: 3 months, 28 days
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Re: lab sterilization [Re: PussyFart]
#19241326 - 12/07/13 02:47 PM (10 years, 2 months ago) |
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 4 hours
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Re: lab sterilization [Re: Ultron]
#19241539 - 12/07/13 03:41 PM (10 years, 2 months ago) |
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Quote:
all the rest is academic thereafter
Perhaps. I'm not aware of anyone actually studying any differences in contamination rate between using a flame in one or not. But so are the reasons for placing the flame source in there in the first place and there are obvious complications in doing so.
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Ultron
alchemist programmer




Registered: 08/04/13
Posts: 751
Loc: inner sanctum of the cosm...
Last seen: 3 months, 28 days
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Re: lab sterilization [Re: Kizzle]
#19241549 - 12/07/13 03:45 PM (10 years, 2 months ago) |
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i wonder if there is a flame less solution for heating tools to red hot
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