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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
Posts: 380
Last seen: 7 years, 5 months
AGAR w/H202
    #19183480 - 11/24/13 04:41 PM (10 years, 4 months ago)

So I had some agar in the PC, which was the last of my agar media that i had and my flask fell down in the PC and spilled everywhere.

Now I know H202 doesn't belong in mycology besides to kill cobweb and disinfect, so don't lecture me I know.

The question is I have a full jar of good media solution I never used because I put hydrogen peroxide in it, I went down to the fridge downstairs and saw that I still had it. It looks very clean and I can see the bubbles frozen in the agar so I figure its sterile. If I were to PC that mason jar could I use that agar, I figure h202 is not very stable and there should be virtually no peroxide left when it's done PCing.

The second option I have is this old bag of agar seaweed I found, it's not powdered, its in like a crystalline form. I have no idea how old it is, but I'm sure I could just grind it up or even dissolve it in water.

I've looked on the forum and have found no answers, please no BS posts.

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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: AGAR w/H202 [Re: nn-IlliniSpiralDMT]
    #19183660 - 11/24/13 05:25 PM (10 years, 4 months ago)

spores or tissue?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
Posts: 380
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Re: AGAR w/H202 [Re: cronicr]
    #19184052 - 11/24/13 06:56 PM (10 years, 4 months ago)

Quote:

cronicr said:
spores or tissue?




I'd be transferring some live tissue from various MS agar plates that I have. I was going to dedicate the whole day to transfers, I got my alcohol lamp and scalpel too:thumbup:

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OfflinePsilicon
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Registered: 08/26/12
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Re: AGAR w/H202 [Re: nn-IlliniSpiralDMT]
    #19184525 - 11/24/13 09:06 PM (10 years, 4 months ago)

Yeah, for transfers I think you'll be just fine.  Spores don't contain catalase, but all macrofungal mycelium does.

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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
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Re: AGAR w/H202 [Re: Psilicon]
    #19184537 - 11/24/13 09:09 PM (10 years, 4 months ago)

Quote:

van der griegen said:
Yeah, for transfers I think you'll be just fine.  Spores don't contain catalase, but all macrofungal mycelium does.




Alright sweet.Thanks:super:

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