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OfflineUltron
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19182758 - 11/24/13 01:51 PM (10 years, 2 months ago)

im a noob and my sterile procedure usually consists of cleaning my sab thoroughly with soapy hot water leaving a lil on the floor of my sab i then wait 5 mins for everything to settle  then wiping everything down with 70% alcohol wipes before putting it into my sab wait another 5 mins and i get my spore syringe and clean it with 70% alcohol and wrap a alcohol wipe around the needle tip and before i inoculate i flame sterilize the needle and in between each jar if i have to put the needle down for any reason i wrap the tip in a fresh alcohol wipe but i try to do it as fast as possible to reduce contam possibility's


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OfflineUltron
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Re: Inoculating safely without a SAB [Re: Ultron]
    #19182763 - 11/24/13 01:53 PM (10 years, 2 months ago)

i do all this in a draft free room that's been cleaned before hand. If i were to take it one step further i probably would build me a tape room


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InvisibleSpitballJedi
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Re: Inoculating safely without a SAB [Re: usnXe6K]
    #19182769 - 11/24/13 01:55 PM (10 years, 2 months ago)

A quick tip from My SAB
Quote:

SpitballJedi said:
Don't get too caught up in exact measurements. You mostly want to go by feel. Every SAB will be slightly different based on your body size.

I simply put the tote in to the position I would mostly be using it in. I sat it on my table and sat in my chair. This is where I'll be doing my sterile work.

Then, I put my arms straight down to my side and then just bent at the elbows so my hands were straight out in front of me. I eyeballed were my middle finger lined up with the tote and made that my holes' center. This is the best way to figure out what the most natural place for the holes should go.

The 2 armholes are shoulder width apart. Some people don't realize how important it is to make the center of the holes about shoulder width apart. This gives it a nice comfortable feel.





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InvisibleusnXe6K

Registered: 12/12/10
Posts: 381
Re: Inoculating safely without a SAB [Re: Ultron]
    #19182773 - 11/24/13 01:56 PM (10 years, 2 months ago)

To inoculate jars? That's amazing. I do it in the kitchen open air. No problems. I've done it at least 10 times and 0 contams. I clean my house regularly, maybe that's why.


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Offlineblueconfusion
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Re: Inoculating safely without a SAB [Re: usnXe6K]
    #19182822 - 11/24/13 02:11 PM (10 years, 2 months ago)

I built a GB when I first started and I hated it so I did open air inoculations for a while and I didn't have any problems at first, but then contams like crazy.  So I dug my box back out to try it again now I love it and will never look back.  It's just a matter of getting comfortable and understanding that success depends on what you put into your projects.


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Offlinekrikkrew
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Re: Inoculating safely without a SAB [Re: blueconfusion]
    #19183027 - 11/24/13 02:58 PM (10 years, 2 months ago)

You can never be too careful.


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Invisibleelasticaltiger
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Re: Inoculating safely without a SAB [Re: usnXe6K]
    #19183490 - 11/24/13 04:43 PM (10 years, 2 months ago)

Quote:

usnXe6K said:
Like I said before. It probably depends on the dirtiness of your inoculation location.




:whateveryousayfreak:

Right because everyone who gets contamination lives in a filthy home with animals running around and shitting everywhere.

Sterile technique obviously has nothing to do with it.


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OfflinePussyFart
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Re: Inoculating safely without a SAB [Re: elasticaltiger]
    #19183502 - 11/24/13 04:45 PM (10 years, 2 months ago)

Obviously....duh!


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