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jpack666



Registered: 10/01/13
Posts: 484
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Strain Isolation for Cakes
#19180320 - 11/23/13 07:59 PM (10 years, 2 months ago) |
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A revival of Johnny Springfield's 2005 thread: How do you guys quickly go from isolate to PF cakes for testing? I'll PM him the link if we get to a different conclusion although he hasn't been seen for over a year... Reason for revival: new information and a secondary question. new members please always use search engine before posting ;-) -----------------
Hi Everyone!
To revive Johnny Springfield's thread, I'll start by setting his context.
He was looking for methods of quickly going from an agar strain isolate to a small test grow, using Cakes.
Final conclusion?
Quote:
RogerRabbit said: The fastest method of testing isolates is to make brf cakes without the verm layer, using a tyvek filter instead. Place a wedge of each strain in each brf cake. Don't waste time making LC(ever) or going to grains if the species you're testing will grow on cakes. RR
This method is a possibility, but is there another way to do it? and if yes, which is better, easier, safer? this is my post revival...
NEW INFORMATION:
I read somewhere that what you could do is simply pull up some warm sterile water in a sterile seringe, then pour that water over your agar to make it turn to liquid.
I think then they mix the surface agar/myc with the tip of the seringe, and then you pull up that liquid mixture in the seringe, which makes an LC in the seringe, that you should then immediatly use to inoculate your jars.
What do you think about this method?
SECONDARY QUESTION:
I was wondering if any of you prefered sticking to cakes and used strain isolation for them. I would think this could be possible for any reason, esthetic, potency, yield, flushes, etc.
I'm kind of interested in making cakes from strain of isolation and was wondering what you thought of this, if it's worth it, if a lot of people do it, etc.
Thanks
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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silverstem
Caps & Stems



Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 5 months
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Re: Strain Isolation for Cakes [Re: jpack666]
#19180390 - 11/23/13 08:17 PM (10 years, 2 months ago) |
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i like RRs method only because it doesn't invite any other ingredients... warm water could contain contams.. thats another sterile thing you have to do...
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Strain Isolation for Cakes [Re: silverstem]
#19180909 - 11/23/13 11:44 PM (10 years, 2 months ago) |
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Yep just as rr described is the only route id think of taking from agar to cake
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Strain Isolation for Cakes [Re: cronicr]
#19180935 - 11/23/13 11:54 PM (10 years, 2 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/9637393#9637393
This reply from RR in a thread called The Truth about Cakes gave me lots of insight on lots of things I needed to clarify.
He's showing again in this thread how cultivated and honest he is.
I'm probably, for my first step, going to start to try and perfect this technique from now on:
agar strain isolation testing on cakes and staying with cakes
Thanks RR (he might not know I just said that)
But seriously, Shroomery + Search Engine + RR's Replies = all you need. I look forward to buying his DVD. Is it his DVD? Not sure!?
Anyways... I think this reply of RR I linked you too is really worth reading if you have time.
Editted in: BTW, there seems to be A LOT of other EXCELLENT teachers around here. Some I've read and others not yet, so I can't start mentioning you all. But as an example, this specific post section from Violet has been one of the most instructive and fun reads I've ever had the chance of finding: http://www.shroomery.org/forums/showflat.php/Number/19035260#19035260
Cheers
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
Edited by jpack666 (11/24/13 12:07 AM)
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tinkerous
get-high ninja



Registered: 04/26/13
Posts: 61
Loc: PNW
Last seen: 8 years, 21 days
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Re: Strain Isolation for Cakes [Re: jpack666]
#19180965 - 11/24/13 12:05 AM (10 years, 2 months ago) |
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yes
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Strain Isolation for Cakes [Re: tinkerous]
#19182036 - 11/24/13 10:34 AM (10 years, 2 months ago) |
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I do exactly as RR describes for my PF-cake-size (~8oz) straight-grain cakes, which are now all I grow by. Of course there's no verm barrier to exclude. There's nothing better than a couple agar wedges to a container, a couple seconds of shake and settling, and Done.
However for PF cakes specifically, and as a good SAB alternative for beginners and learners, I suggest doing what your OP's question was asked of. Water suspension (some call 'slurry') I very much dislike doing it from petri dishes; it still requires clean work in SAB or sterile airflow and surface-wiping with a needle... might as well go straight agar wedge. However- From and To sealed containers with injection options it is an incredible way to use syringes to make whatever transfers with entirely enclosed sterile tools. Works better and is less tedious with with larger syringes and needles, with a small torch lighter.
Flame needle on asterilized syringe, suck up water from a container/jar that has been sterilized with a filtered and ported lid. Flame needle, inject water into colonized no-contam agar container or assuredly no-contam grain jar, hold the top of jar and attached needle firmly with one hand, and shake gently then increasingly harder to break up and loose surface mycelium only as much is necessary, will vary. Suck mycelium water back into syringe. Flame needle and inoculate as you like!
This allows liquid inoc of PF cakes which will run down all 4 sides and colonize the whole cake very quickly. This is the fastest way to colonize PF cakes, especially the regular half-pints, Period.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
Edited by Violet (11/24/13 01:29 PM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Strain Isolation for Cakes [Re: jpack666]
#19182236 - 11/24/13 11:28 AM (10 years, 2 months ago) |
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My opinion:
Do it like RR describes. It's the easiest way. Making syringes adds more steps which increases the possibility of contaminating your work.
even if LC would colonize your cake faster than agar, it's not gonna be so much faster to make it worth the extra effort and risk.
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