Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineg3n3h4x0r
Fungal Geneticist

Registered: 09/29/13
Posts: 73
Last seen: 8 years, 9 months
Tek Swap - Quick question
    #19179418 - 11/23/13 03:39 PM (10 years, 2 months ago)

I've been doing BRF tek lately and it has been great.  I've been thinking of swapping to a grain tek - maybe rye berries or something - and I came up with a thought I wanted to run by, not really sure if it's feasible:

I was thinking about cutting up my BRF cakes to get to the core of the cake, and inoculating the grain jars with that (similar protocol to a grain-to-grain transfer).  I'm just wondering peoples' thoughts.  I usually retire cakes when they stop producing like I'd want them to (clear of contaminants), and I would think the interior of the cake would be fairly clean.  I don't really have the experience to answer this, though.

I have spores and LC - I'm just sort of curious to how/if you think this would work.  Thanks!  :]


Extras: Filter Print Post Top
Offlinejoshfor20
boss
Male


Registered: 10/19/13
Posts: 24
Loc: on a mountain
Last seen: 9 years, 10 months
Re: Tek Swap - Quick question [Re: g3n3h4x0r]
    #19179445 - 11/23/13 03:50 PM (10 years, 2 months ago)

It might work. You might also try getting a sample from a stem. That works well. It also isolates one strain from your multi-spore inoculation.


--------------------
Live life!!


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: g3n3h4x0r]
    #19179493 - 11/23/13 04:07 PM (10 years, 2 months ago)

Chance of success is pretty low. You will have a difficult time getting a clean sample from a cake.

Even if you open your jar in a SAB, you still have the contam barrier to deal with.

Soaking will add more contaminants and fruiting exposes it even further more.

Plus,

Sterilizing doesn't kill everything. Eventually, whatever survives the sterilization and/or comes in contact with that cake will eventually germinate and grow.

Over time, as the myc weakens, your chance of seeing contams will increase.

Just because you can't see contams does not mean they are absent. We generally only refer to them as contams once they affect our grow.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Offlinenksfo5
traverse traveler


Registered: 12/19/12
Posts: 689
Last seen: 8 months, 14 days
Re: Tek Swap - Quick question [Re: joshfor20]
    #19179507 - 11/23/13 04:11 PM (10 years, 2 months ago)

i tried this about two weeks ago, the mycelium from the cake grabbed ahold of the rye grains really well and new growth was initiated about a day or day and a half after i placed them the chunks of mycelium into the rye jar. although the mycelium did well grabbing on to the rye i did not use a GB or a SAB and only sanitized as best as i could and attempted things this way. the first few days things were going great new growth and no contams. but about a week later i was unpleased to find that it was not working and that my rye grain was covered in a green fuzz.

i did a little bit of research on the forum here but not much into this subject matter. i decided against trying again after that as open air has never been a problem for me before.


Extras: Filter Print Post Top
Offlinejoshfor20
boss
Male


Registered: 10/19/13
Posts: 24
Loc: on a mountain
Last seen: 9 years, 10 months
Re: Tek Swap - Quick question [Re: nksfo5]
    #19179588 - 11/23/13 04:35 PM (10 years, 2 months ago)

A still air box really is necessary to get by at this level of mycology. Other than a flow hood of course.


--------------------
Live life!!


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Tek Swap - Quick question [Re: joshfor20]
    #19179623 - 11/23/13 04:45 PM (10 years, 2 months ago)

yea def go SAB. no excuse not to. cardboard and saran wrap if u have to!  My first sab was just my laundry tote cleaned out and turned upside down over the lip of the table.  now its a laundry tote with a lid and 2 holes.  not hard.  its not fun waiting 2 weeks with good growth then a contam hits.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Offlineg3n3h4x0r
Fungal Geneticist

Registered: 09/29/13
Posts: 73
Last seen: 8 years, 9 months
Re: Tek Swap - Quick question [Re: blindingleaf]
    #19183771 - 11/24/13 05:53 PM (10 years, 2 months ago)

Awesome.  I thought contams would be the biggest issue - I was mostly inquiring out of curiosity. 

Quote:

i tried this about two weeks ago, the mycelium from the cake grabbed ahold of the rye grains really well and new growth was initiated about a day or day and a half after i placed them the chunks of mycelium into the rye jar. although the mycelium did well grabbing on to the rye i did not use a GB or a SAB and only sanitized as best as i could and attempted things this way. the first few days things were going great new growth and no contams. but about a week later i was unpleased to find that it was not working and that my rye grain was covered in a green fuzz.




Thanks for the personal experience!

I'll avoid creating the scenario, then.  I've done plenty of work on various bacteria as well, and it's off putting that fungi are much more prone to failure due to contamination.  Sort of a disappointment how efficient molds are and we're practically setting up perfect conditions to grow the mold as well as any fungi of interest...  x_x

Thanks, everyone!


Extras: Filter Print Post Top
InvisibleSmeagol
Poke my 3rd eye
Male User Gallery

Registered: 04/14/13
Posts: 319
Re: Tek Swap - Quick question [Re: g3n3h4x0r]
    #19184202 - 11/24/13 07:28 PM (10 years, 2 months ago)

taking a chunk of myc from that cake or a chunk of stem (clone) will not isolate a strain if they were inoculated with a multi spore solution.  It takes transering from agar to agar a couple times until the mycelium grows in "sectors". a sample from a single sector is an isolate.  even cloning a single fruit from a ms won't isolate the genetics.


--------------------
The road to hell is paved with good intentions, but it is not paved with clarity.” -Terence

"If you're not peaking what the fuck are you doing?" Dude on facebook


Extras: Filter Print Post Top
Offlinejoshfor20
boss
Male


Registered: 10/19/13
Posts: 24
Loc: on a mountain
Last seen: 9 years, 10 months
Re: Tek Swap - Quick question [Re: Smeagol]
    #19187930 - 11/25/13 05:15 PM (10 years, 2 months ago)

Quote:

Smeagol said:
taking a chunk of myc from that cake or a chunk of stem (clone) will not isolate a strain if they were inoculated with a multi spore solution.  It takes transering from agar to agar a couple times until the mycelium grows in "sectors". a sample from a single sector is an isolate.  even cloning a single fruit from a ms won't isolate the genetics.


I am pretty sure cloning from a stem isolates one Dikaryotic strain without the need for agar.


--------------------
Live life!!


Extras: Filter Print Post Top
Offlinejoshfor20
boss
Male


Registered: 10/19/13
Posts: 24
Loc: on a mountain
Last seen: 9 years, 10 months
Re: Tek Swap - Quick question [Re: joshfor20]
    #19187974 - 11/25/13 05:25 PM (10 years, 2 months ago)

Then again i have been wrong. I Clone, I don't isolate


--------------------
Live life!!


Extras: Filter Print Post Top
Offlinesilverstem
Caps & Stems
Male


Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Tek Swap - Quick question [Re: joshfor20]
    #19188038 - 11/25/13 05:40 PM (10 years, 2 months ago)

Quote:

joshfor20 said:
Quote:

Smeagol said:
taking a chunk of myc from that cake or a chunk of stem (clone) will not isolate a strain if they were inoculated with a multi spore solution.  It takes transering from agar to agar a couple times until the mycelium grows in "sectors". a sample from a single sector is an isolate.  even cloning a single fruit from a ms won't isolate the genetics.


I am pretty sure cloning from a stem isolates one Dikaryotic strain without the need for agar.



cloning from a stem insures the mushroom you picked will be in the genetics of the myc that grows from it... you will need agar for this... isolating from a strain could mean that you take out partial genetics from that mushroom and results will very...


--------------------


Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: silverstem]
    #19188153 - 11/25/13 06:04 PM (10 years, 2 months ago)

I have cloned mushrooms that did not sector, but it is not a common occurrence.


Extras: Filter Print Post Top
Offlinejoshfor20
boss
Male


Registered: 10/19/13
Posts: 24
Loc: on a mountain
Last seen: 9 years, 10 months
Re: Tek Swap - Quick question [Re: silverstem]
    #19188164 - 11/25/13 06:05 PM (10 years, 2 months ago)

Quote:

silverstem said:
Quote:

joshfor20 said:
Quote:

Smeagol said:
taking a chunk of myc from that cake or a chunk of stem (clone) will not isolate a strain if they were inoculated with a multi spore solution.  It takes transering from agar to agar a couple times until the mycelium grows in "sectors". a sample from a single sector is an isolate.  even cloning a single fruit from a ms won't isolate the genetics.


I am pretty sure cloning from a stem isolates one Dikaryotic strain without the need for agar.



cloning from a stem insures the mushroom you picked will be in the genetics of the myc that grows from it... you will need agar for this... isolating from a strain could mean that you take out partial genetics from that mushroom and results will very...


yep, after i posted that i knew i didn't sound right. Typically im just happy if i have all rhizomorphic growth.


--------------------
Live life!!


Extras: Filter Print Post Top
Offlinesilverstem
Caps & Stems
Male


Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Tek Swap - Quick question [Re: Pastywhyte]
    #19188172 - 11/25/13 06:06 PM (10 years, 2 months ago)

if you make a less nutritious agar your myc will become rhyzomorpic and will most likely sector.


--------------------


Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: silverstem]
    #19188183 - 11/25/13 06:08 PM (10 years, 2 months ago)

Quote:

silverstem said:
if you make a less nutritious agar your myc will become rhyzomorpic and will most likely sector.




I am aware of that. . . Not all fruits are comprised of multiple strains. It is rare but can happen.


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: Pastywhyte]
    #19188667 - 11/25/13 07:32 PM (10 years, 2 months ago)

Almost all, if not all, fruits of a MS grow will have multiple genetics. Sometimes it takes more than one transfer on agar of a MS fruit to have visible sectoring.

Rhyzomorphic mycelial growth does not indicate fruit characteristics.

You could take your best fruit from a MS grow and isolate the genetics on agar. Take the most rhyzomorphic of the isolates and still end up with a non-fruiter.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: SpitballJedi]
    #19188712 - 11/25/13 07:40 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Almost all, if not all, fruits of a MS grow will have multiple genetics. Sometimes it takes more than one transfer on agar of a MS fruit to have visible sectoring.




I know. I have probably taken 50+ clones in the last year alone. But I have had a clone that would not sector, no matter how many times I transferred or how nutritious that agar was. I never once said it was a common occurrence. Maybe its because it was a pin that formed invitro on a plate that was the 6th transfer and was starting to sector :shrug: It was rhizomorphic as hell. I never did try to fruit it, just transferred to a slant and forgot about it until a few weeks ago. I now have it colonizing a master, hoping it fruits as good as it looks
:ohsodevious:


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Tek Swap - Quick question [Re: Pastywhyte]
    #19188746 - 11/25/13 07:47 PM (10 years, 2 months ago)

My bad. I thought you were saying it's rare to have multiple genetics in a fruit. I've been drinking, so I'm a little slow.

Yeah, you may have got an isolate from that invetro pin. It happens.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* New to the boards, and a quick question to start ! Anonymous 1,376 4 03/11/02 01:18 AM
by 2shrewmen
* quick question SuppaFly 830 1 09/18/02 02:41 PM
by mickey_rourke
* Re: Quick Question
( 1 2 all )
Anonymous 3,693 22 06/14/00 03:46 AM
by hitterg
* Quick question about quart/pint jars liquidplaya 2,543 18 09/20/01 06:03 PM
by darkfly
* some quick questions about my treasure coasts alf 3,248 19 11/15/01 11:54 PM
by Anonymous
* Quick Question, quick answer please Close Call 560 1 10/06/02 10:18 AM
by rheidiohed121
* Quick Question LXTC 1,342 11 09/24/02 08:09 PM
by LXTC
* a few quick questions about humidity toadstool82 1,454 5 07/02/02 05:52 PM
by GreyMatter

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,078 topic views. 39 members, 220 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.036 seconds spending 0.012 seconds on 14 queries.