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mycomattie



Registered: 11/15/12
Posts: 1,323
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PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub *UPDATE 12/26 - I Fucked Up*
#19179385 - 11/23/13 03:28 PM (10 years, 3 months ago) |
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11/13/13
Earlier this month, I noticed I had a quart of PE on a shelf, fully colonized, since mid September. It was inoculated with an agar wedge containing a PE isolate.
As you can see in the pic - it's very dehydrated and bruised blue from me trying to beat it/break it up a bit for the grain to gran transfer.

I had 3 empty quart jars available; so I prepped them with Rye Grass Seed (soaked RGS/gypsum/diluted coffee/boiling water for ~ 5 hours) and PC'd at 15psi, for 90 minutes.
 
I'm just curious if I can get a good grow from what are essentially spare supplies and 1 old quart jar.
11/14/13
Just G2G'd - which sucked, since the mycelium had consolidated to such a point that it was breaking off in large chunks (which is why G2G is recommended at exactly 100% colonization). I used a disposable scalpel that I usually use for agar. My SAB which isn't fancy, but has been pretty reliable.
 
The jars after inoculated.

II'm hoping I can get a good grow from what are essentially spare supplies and 1 old quart jar. We shall see...
11/20/13 Ah the joys of home mycology - patience... This PE was originally from a MS syringe and it's overall colonization speed is SLOW - even when the rhizomorphic sectors were isolated.
  11/23/13
Shook them this morning. For whatever reason, shaking doesn't seem to work to well with these PE's - the mycelium consolidates and stays chunky, which makes it hard to break up and spread throughout the quart. The good thing about RGS - it creates thousands of inoculation points.
I'll update as I go
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Edited by mycomattie (12/26/13 11:54 AM)
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shaneh79
Stranger

Registered: 09/02/13
Posts: 11
Last seen: 9 years, 11 months
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19179940 - 11/23/13 06:09 PM (10 years, 3 months ago) |
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Cool how you can create so much from such small beginnings in musicology. I am working on my first isolation of Mexi cub right now I'm excited! Thanks for posting your grow!
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: shaneh79]
#19180001 - 11/23/13 06:28 PM (10 years, 3 months ago) |
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Quote:
shaneh79 said: Cool how you can create so much from such small beginnings in musicology. I am working on my first isolation of Mexi cub right now I'm excited! Thanks for posting your grow!
Definitely my friend - I'm new to agar/isolation myself, so best wishes and good vibes on your mycological endeavors.
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19187928 - 11/25/13 05:15 PM (10 years, 2 months ago) |
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11/25/13
Well, It's coming along, but that may be my last shake for this PE - it definitely seems like it may be slowing the colonization; not speeding it up
Latest pics...
 
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silverstem
Caps & Stems



Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 6 months
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19188090 - 11/25/13 05:51 PM (10 years, 2 months ago) |
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you have to allow time for your myc to recover after shaking... it will slow growth but growth speed will increase after recovery.
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: silverstem]
#19188193 - 11/25/13 06:11 PM (10 years, 2 months ago) |
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Quote:
silverstem said: you have to allow time for your myc to recover after shaking... it will slow growth but growth speed will increase after recovery.
Very true - it's just this PE is slower than anything I've experienced in the past............but, better than the "C" word; at least the mycelium looks healthy.
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silverstem
Caps & Stems



Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 6 months
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19188197 - 11/25/13 06:13 PM (10 years, 2 months ago) |
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yea it looks very healthy...
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: silverstem]
#19189370 - 11/25/13 10:14 PM (10 years, 2 months ago) |
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Quote:
silverstem said: yea it looks very healthy...
Thanks, hopefully it stays that way haha - I'll take slow colonization over contams though :\
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19204509 - 11/29/13 11:24 AM (10 years, 2 months ago) |
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11/29/13 Well, the jars are coming along - I'd say, as of today, they're ~ 70 - 80% colonized. Definitely testing my patience.

On a side note - I have several agar projects going on.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19204537 - 11/29/13 11:35 AM (10 years, 2 months ago) |
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shake shake shake?
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: spacechildo]
#19246809 - 12/08/13 07:37 PM (10 years, 2 months ago) |
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12/8/13
Spawned to bulk in a monotub. Actually yesterday, but I took pictures today.
 
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19247002 - 12/08/13 08:17 PM (10 years, 2 months ago) |
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Good stuff mattie I gonna watch this one
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: Pastywhyte]
#19249124 - 12/09/13 08:20 AM (10 years, 2 months ago) |
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Thank you man!
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bootster

Registered: 02/22/11 
Posts: 1,531
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19249590 - 12/09/13 11:09 AM (10 years, 2 months ago) |
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Good job.
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: bootster]
#19256689 - 12/10/13 06:01 PM (10 years, 2 months ago) |
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Thanks everyone.
11/18/13
The monotub is colonizing nicely (yup, I open the lid to check every few days, which I know is considered "bad practice").
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19256710 - 12/10/13 06:05 PM (10 years, 2 months ago) |
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I don't consider it bad practice, I do it all the time. Nice looking tub BTW.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: Pastywhyte]
#19256714 - 12/10/13 06:06 PM (10 years, 2 months ago) |
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everyday is bad practice, every few is good parenting
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: cronicr]
#19257374 - 12/10/13 08:00 PM (10 years, 2 months ago) |
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Cool - thanks guys!
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19319985 - 12/24/13 07:48 AM (10 years, 2 months ago) |
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12/24/13
Fruiting was initiated 10 days ago, and today I see the very first pin set. I'm curious to see the overall results, since this is my first time casing cubensis.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: PE Grow Log: Agar Isolate to Rye Berries -> G2G -> Monotub [Re: mycomattie]
#19320716 - 12/24/13 11:41 AM (10 years, 2 months ago) |
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Sweet!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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