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Invisibleblindingleaf
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Re: Think I might have sterilized my sub [Re: blindingleaf]
    #19636518 - 03/01/14 07:41 PM (9 years, 10 months ago)

i've got enoki on sterilized coir now, but its not liking it as much as sawdust



coir on left, 2 sawdust to the right of it


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Offlineinvitro


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Re: Think I might have sterilized my sub [Re: FrankHorrigan]
    #19636568 - 03/01/14 07:51 PM (9 years, 10 months ago)

Quote:

FrankHorrigan said:
Why is there a question? It's coir. Coir works sterilized, bucketed, or pasteurized. There's no real difference with the prep for the coir so long as it colonizes, and all three methods will be fine if you have clean grain spawn.

RR, myself, cronicr, pasty, have all been saying this :shrug:





In this thread: http://www.shroomery.org/forums/showflat.php/Number/19619773#19619773
RR said "the bucket tek is bunk" referring to coir.  That was less than a week ago.


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InvisibleFrankHorrigan
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Re: Think I might have sterilized my sub [Re: invitro]
    #19636583 - 03/01/14 07:54 PM (9 years, 10 months ago)

You missed the context apparently. Bucket tek is a bunk method of pasteurizing, but it works for preparing coir.

Quote:

RogerRabbit said:
Bucket tek is bunk, but that's not the problem.  Your spawn was contaminated.  Coir will not contaminate before first flush unless you spawn grains with molds to it.  Fix what is broken first.
RR




Quote:

RogerRabbit said:
Perhaps with coir the best term is cooked.  Even when left untreated, mold spores rarely germinate on plain coir.  However, it's also piss-poor for mushroom mycelium as those who have used un-pasteurized coir for mushroom growing can attest.  I suspect it has something to do with making the cells easier for the mycelium to digest, much like the effect we humans get from cooking our food.

In that light, coir requires neither pasteurization nor sterilization, even though both seem to perform equally well, as does tossing it into a bucket with some boiling water.
RR




(bold added for emphasis :wink: )


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You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (03/01/14 08:00 PM)


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Offline1down5up
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Re: Think I might have sterilized my sub [Re: FrankHorrigan]
    #19638345 - 03/02/14 06:19 AM (9 years, 10 months ago)

This tub was spawned to sterilized CVG, and then cased with the same.  If there was anything wrong with it, i would've known by now. 



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~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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InvisibleSpitballJedi
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Re: Think I might have sterilized my sub [Re: invitro]
    #19638830 - 03/02/14 10:02 AM (9 years, 10 months ago)

Quote:

invitro said:
Quote:

FrankHorrigan said:
Why is there a question? It's coir. Coir works sterilized, bucketed, or pasteurized. There's no real difference with the prep for the coir so long as it colonizes, and all three methods will be fine if you have clean grain spawn.

RR, myself, cronicr, pasty, have all been saying this :shrug:





In this thread: http://www.shroomery.org/forums/showflat.php/Number/19619773#19619773
RR said "the bucket tek is bunk" referring to coir.  That was less than a week ago.




Yes, people do make seemingly contradictory statements. I have found the best way to clarify these episodes is through experimentation. I don't always have a good scientific explanation for why stuff happens, but I get the job done.

Pasteurization and sterilization of CVG definitely work and are my preferred methods.

I still have my doubts about the reliability of just throwing boiling water on CVG in a bucket. But, I digress:
Official Bucket Tek vs Pasteurization Thread


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InvisibleFrankHorrigan
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Re: Think I might have sterilized my sub [Re: SpitballJedi]
    #19638926 - 03/02/14 10:31 AM (9 years, 10 months ago)

If you can use sterile coir spawned in open air, bucket coir is going to be no problem.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisibleSpitballJedi
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Re: Think I might have sterilized my sub [Re: FrankHorrigan]
    #19638938 - 03/02/14 10:35 AM (9 years, 10 months ago)

Have you gone back to bucket tek?


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A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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InvisibleFrankHorrigan
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Re: Think I might have sterilized my sub [Re: SpitballJedi]
    #19639004 - 03/02/14 11:02 AM (9 years, 10 months ago)

I mean, I haven't "gone back" to it. But it works fine for coir and verm alone.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisiblePastywhyteMDiscord
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Re: Think I might have sterilized my sub [Re: FrankHorrigan]
    #19639126 - 03/02/14 11:47 AM (9 years, 10 months ago)

Some damn good discussion here,  can't wait to see your bucket results Frank :awesomenod:


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InvisibleFrankHorrigan
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Re: Think I might have sterilized my sub [Re: 1down5up]
    #19639132 - 03/02/14 11:49 AM (9 years, 10 months ago)

Quote:

1down5up said:
If there was anything wrong with it, i would've known by now.




Don't say that til you harvest your first flush and see the start of the second, trust me, I speak from experience.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offline1down5up
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Re: Think I might have sterilized my sub [Re: FrankHorrigan]
    #19640384 - 03/02/14 04:30 PM (9 years, 10 months ago)

good point frank...and i do trust you, everything i've read:thumbup:
first flush is over, will soak tomorrow or the next day.  I'll keep ya's posted.


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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Offlinetombosley8
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Re: Think I might have sterilized my sub [Re: 1down5up]
    #19659635 - 03/06/14 02:49 PM (9 years, 10 months ago)

so would my mycelium be just as contam resistant before and after 1st flush using "cooked" probably sterilized unless you think bucket tek will work with coir verm and gypsum(have had my brick not fully hydrated a few times leaving solid dry chunks in my sub) as pasteurized or should i leave something out(gypsum, maybe verm too) In other words do you know if the two(pasteurization and sterilizing) work exactly identical with these three ingredients coir/verm and gypsum

and does a pasteurized colonized substrate last longer than a sterilized in terms of flushes and contam


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Edited by tombosley8 (03/06/14 02:51 PM)


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Offline1down5up
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659756 - 03/06/14 03:27 PM (9 years, 10 months ago)

Excellent question...I don't know the answer to that one


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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InvisiblebodhisattaMDiscordReddit
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Re: Think I might have sterilized my sub [Re: 1down5up]
    #19659831 - 03/06/14 03:48 PM (9 years, 10 months ago)

beats me but after the first flush you're still fully colonized so it should be just as contam resistant aside from being older. The bigger issues to worry about are having a good fast culture that colonizes in the 10 day range, having your field capacity perfect, having lots of fresh air, and not destroying your substrate when you harvest.


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Offlinetombosley8
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659865 - 03/06/14 04:01 PM (9 years, 10 months ago)

Does any one use a large container with a steamer to sterilize/cook?


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Offlinetombosley8
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659871 - 03/06/14 04:03 PM (9 years, 10 months ago)

are you saying if not colonized in 10 days a non pasteurized is likely to contam or does this go for pasteurized sub as well?


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Invisibleblindingleaf
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659879 - 03/06/14 04:06 PM (9 years, 10 months ago)

i tried pasteurizing in a large 5 lb bucket in my PC  once.  i use them to grow in, and figured it would be perfect way to measure out amt of substrate i need.  its pp5 plastic meant for holding tuna and chicken salad in deli i work in.  even after 1 hour, the temp in center had climbed barely 9 degrees.  with glass and bags, it was taking 24-30 minutes to get up 20 degrees.  not sure why that happened, or if it would happen to u, but glass heats up and holds its heat better than plastic, and bags are so thin, even if u have a lot of substrate in them, the heat from the water is nearly touching them, for all intensive purposes.  so just be careful with large containers if they are plastic, make sure u got a thermometer in there.  have not tried these containers with PC pressure cycle tho.


--------------------
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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisibleblindingleaf
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659887 - 03/06/14 04:09 PM (9 years, 10 months ago)

Quote:

tombosley8 said:
are you saying if not colonized in 10 days a non pasteurized is likely to contam or does this go for pasteurized sub as well?




not necessarily if ur spawn is good.  goes for any substrate though that if its partly uncolonized it is susceptible to con tams .

if u are using non treated substrate, it will likely take longer than 10 days, and may not finish at all.  i tried it once with plain coir.  colonized 50% then stopped, but did not contam before i threw it out (i kept it closed with just GE holes)


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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Re: Think I might have sterilized my sub [Re: blindingleaf]
    #19659896 - 03/06/14 04:12 PM (9 years, 10 months ago)

I meant like a 30 gal resevoir with a wagner steamer hose hooked up to bottom and say a rack with bags of sub(obviously coir sub probobly around 5 qts each)spaced appropriately and let run for a few hours with a near seal on the lid to allow as little steam escape as possible.


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Invisibleblindingleaf
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Re: Think I might have sterilized my sub [Re: tombosley8]
    #19659920 - 03/06/14 04:21 PM (9 years, 10 months ago)

yea that would work b/c u wouldn't need to worry about temps getting over 160 with coir/verm/gyp.  i mean as long as its getting steamed for an extended period, and not just hot water thats steaming a little, it should work :shrug:


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MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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