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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineRevolted627
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Half my jars showing growth, Half are not.
    #19178290 - 11/23/13 10:10 AM (10 years, 3 months ago)

Hello everyone this is my first post!

Ok so I recently started the PF Tek. I made my  20 BRF jars to the standard, and PC'ed 5 at a time for 60 minutes each at 15 PSI. I let them all cool for plenty of time during inoculation. I used a small disinfected room with a glove box, rubber gloves, face mask, and rubbing alcohol to inject my jars. I originally was going to flame sterilize my needle with my butane touch, but it crapped out on me. So for the first 10 jars (call them Jars A) I used a regular BIC lighter to heat the syringe for the first jar, but I did not like the black stuff it was leaving on the needle, so from here on out I just rubbed needle with alcohol and wiped dry. So when it came to the second 10 Jars (Jars B) I just wiped in alcohol and dried needle before each jar. After this I put Jars in a TiT. Jars A were on the bottom, Jars B on the top, separated by a little rack. During the first week I checked the temperature several times. One of these time the temp reached 82.2 on the top half of the tote. No telling how hot it was on the bottom half were jars A were located. I checked the jars almost a week later and all of Jars B have signs of healthy growth. All of Jars A have zero signs of growth. I since moved them out of the TiT and they are just in a regular tote with room temp.

So I figure there are only a few options here... either 1. I had one good syringe and one bad syringe. 2. They heat on the bottom half of the TiT halted or killed the mycelium. 3. Something I did during the injections killed the mycelium.

Any advice would be appreciated


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OfflineCasual Cultivator
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Registered: 06/25/12
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Re: Half my jars showing growth, Half are not. [Re: Revolted627]
    #19178346 - 11/23/13 10:24 AM (10 years, 3 months ago)

I flame sterilize after each jar... dont wipe with alcohol, also the black stuff is ok, it is all sterile. And as for your jars i doubt you killed your mycellium but i would recommend leaving on a shelf opposed to anything else. Only time will tell :\


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OfflineRevolted627
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Re: Half my jars showing growth, Half are not. [Re: Casual Cultivator]
    #19178383 - 11/23/13 10:31 AM (10 years, 3 months ago)

I understand that I should have flame sterilized between jars but it is too late for that. I did however order a nice alcohol lamp for the future.


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Offlinetinkerous
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Re: Half my jars showing growth, Half are not. [Re: Revolted627]
    #19178456 - 11/23/13 10:49 AM (10 years, 3 months ago)

I made an alcohol burner from a mason jar, but ended up preferring the torch instead.


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OfflineRevolted627
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Re: Half my jars showing growth, Half are not. [Re: tinkerous]
    #19178978 - 11/23/13 01:24 PM (10 years, 3 months ago)

Quote:

tinkerous said:
I made an alcohol burner from a mason jar, but ended up preferring the torch instead.




Yeah I have a nice torch too. The turboflame arc. It normally runs great but it decided to mess with me when I was elbows deep in my glove box :frown:


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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


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