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OfflineTerry M
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Identify the Zurich Mystery Culture -- pics!
    #19178093 - 11/23/13 08:56 AM (10 years, 2 months ago)

Win valuable prizes!

You see, I got this unmarked culture mailed from Zurich, Switzerland, and I don't know what edible it is or who sent it. I do a lot of culture trading, and this one must have escaped my records.

Here's what it looks like on agar after 8 days:

 

 

Any guesses?


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OfflineJeff
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19178840 - 11/23/13 12:47 PM (10 years, 2 months ago)

Beech was the first thing to pop into my head Terry. 
Probably totally off.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Jeff]
    #19179076 - 11/23/13 01:57 PM (10 years, 2 months ago)

Hard to say. Maybe C. comatus, or morchella sp.?
Curious to see what is turns out to be. :strokebeard:


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OfflineForresterM
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Versicolor]
    #19179138 - 11/23/13 02:12 PM (10 years, 2 months ago)

I'm going with pink oyster.


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Forrester]
    #19179167 - 11/23/13 02:18 PM (10 years, 2 months ago)

What about the rings? And the slightly powdery surface? They've got me confused.


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OfflineJeff
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19179277 - 11/23/13 02:54 PM (10 years, 2 months ago)

Have you thrown it under your scope yet?


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Jeff]
    #19179446 - 11/23/13 03:50 PM (10 years, 2 months ago)

Quote:

Jeff said:
Have you thrown it under your scope yet?




Nope. I don't think I'll see much from the bottom, and my binocular scope objective, even the lowest power, won't take me down low enough to focus the agar surface. When I try this, I crack about 5% of plates, even when I remind myself to turn the focus knob in only one direction!

I don't have a stereo microscope, which I'd really need to stay up high enough with the cover on (at lower power, of course). On my next next sterile lab day, I can make a spare plate which I can take the cover off of, thereby contaminating it, but allowing me to see the mycelium with my binocular microscope. Odds are, it will look like generic mycelium, but you never know! :shocked:


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InvisibleVersicolor
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19179649 - 11/23/13 04:50 PM (10 years, 2 months ago)

I have broken so many dishes with my microscope. It can get a clear image if I push it as close as it gets. So frustrating. :lol:


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InvisibleZen Peddler
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19179705 - 11/23/13 05:05 PM (10 years, 2 months ago)

Looks wispy like Lepista nuda but those rings I've seen in several oyster species when the strain is pretty old or sectoring weirdly.
I've got shaggymanes here right now and its quite different well the strain I have is anyway.
so my bet / guess is an old king oyster or pearl oyster


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OfflineForresterM
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19179840 - 11/23/13 05:44 PM (10 years, 2 months ago)

So what's the plan?  Spawn some grain to sawdust and see if you get any more clues or pins?


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Forrester]
    #19179893 - 11/23/13 05:58 PM (10 years, 2 months ago)

My guess is Flammulina velutipes. 

You should make this a contest terry- since dozens of competent mycologists have looked at this thread and no one seems to have a good idea. 

I'm looking forward to receiving my wedge when you fruit out a wild cloned velvet shank.


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Edited by laughingsol (11/23/13 06:01 PM)


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InvisibleVersicolor
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Re: Identify the Zurich Mystery Culture -- pics! [Re: laughingsol]
    #19180078 - 11/23/13 06:45 PM (10 years, 2 months ago)

Isn't Lepista nuda myc reddish?
I've never seen an oyster with that type of mycelium, but maybe it's some wild swiss strain that looks different.
I would be interested to see what the powdery texture looks like under a microscope. I've seen microsclerotia on morel myc, but I doubt that's what you have.


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Versicolor]
    #19180303 - 11/23/13 07:55 PM (10 years, 2 months ago)

Yes, all the Lepista nuda I've grown develop a pinkish hue to the mycelium.

I guess I'll make some grain jars and then to sawdust bags. And I'll make it a contest -- with a prize!


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InvisibleZen Peddler
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19180373 - 11/23/13 08:12 PM (10 years, 2 months ago)

According to Stamets Lepista nuda has white bluish mycelium. I'm currently working with 12 different isolates of Lepista nuda right now - mainly Australian strains isolated from multispore swipes of wild prints but some from Washington state and all of them are whitish grey tormentose and slightly aerial mycelia that turns a subtle blue purple when it covers the supplemented MEA.
none are even close to red or pink (unless you call dark lilac pink) and the only supposed red mycelia of any Lepista species I've seen (nuda or saeva) was a guy on this forum and undoubtedly he was growing and sectoring a mold colony :smile:


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19180381 - 11/23/13 08:14 PM (10 years, 2 months ago)

Lepista nuda is my interest species - I'm obsessed with it.

But back to your mystery culture - I'm going with pearl or king oyster. Seems a boring guess but those rings are familiar


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19180388 - 11/23/13 08:16 PM (10 years, 2 months ago)

I've never grown or seen a velvet shank or their mutated white cultivated counterparts so that seems a good guess from above


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InvisibleFoxFire
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Re: Identify the Zurich Mystery Culture -- pics! [Re: laughingsol]
    #19180644 - 11/23/13 09:46 PM (10 years, 2 months ago)

Oooh fun!  I'm guessing Enoki as well.
:havesomescience:

-FF


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OfflineForresterM
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19180669 - 11/23/13 09:59 PM (10 years, 2 months ago)

Quote:

bluemeanie said:
Lepista nuda is my interest species - I'm obsessed with it.

But back to your mystery culture - I'm going with pearl or king oyster. Seems a boring guess but those rings are familiar




Haha, not to get too OT but isn't clitocybe nuda the current name?

I'm never one to argue taxonomy, but... I like to poke sometimes :wink:


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Forrester]
    #19180731 - 11/23/13 10:27 PM (10 years, 2 months ago)

Yes Clitocybe nuda is correct - I prefer Lepista myself - its hard to tell average people you're growing a clitocybe.
Fantastic flavored mushrooms. The Aussie strains run faster than the rest.


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OfflineAmanita virosa
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19180744 - 11/23/13 10:33 PM (10 years, 2 months ago)

I am going with Shaggy mane. I have a blewet that is quite blue colored mycelium.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Amanita virosa]
    #19180850 - 11/23/13 11:22 PM (10 years, 2 months ago)

Could well be. I've only ever had one strain of that and it was pretty weird


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InvisibleVersicolor
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19181581 - 11/24/13 06:51 AM (10 years, 2 months ago)

Quote:

bluemeanie said:
I prefer Lepista myself - its hard to tell average people you're growing a clitocybe.




:lmafo: Same here. I just like to pretend it's still Lepista.


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OfflineForresterM
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19181590 - 11/24/13 06:55 AM (10 years, 2 months ago)

Quote:

bluemeanie said:
Yes Clitocybe nuda is correct - I prefer Lepista myself - its hard to tell average people you're growing a clitocybe.





:lol: so true


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Versicolor]
    #19181705 - 11/24/13 08:10 AM (10 years, 2 months ago)

Quote:

Versicolor said:
Quote:

bluemeanie said:
I prefer Lepista myself - its hard to tell average people you're growing a clitocybe.




:lmafo: Same here. I just like to pretend it's still Lepista.




:laugh:
I'm usually a stickler for using the correct, up-to-date Latin name, but so many people still know this as Lepista. However, I maintain a master list of all my cultures, and I've got this listed as "Lepista (Clitocybe) nuda."


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OfflineAmanita virosa
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19181777 - 11/24/13 08:50 AM (10 years, 2 months ago)

Quote:

Terry M said:
Quote:

Versicolor said:
Quote:

bluemeanie said:
I prefer Lepista myself - its hard to tell average people you're growing a clitocybe.




:lmafo: Same here. I just like to pretend it's still Lepista.




:laugh:
I'm usually a stickler for using the correct, up-to-date Latin name, but so many people still know this as Lepista. However, I maintain a master list of all my cultures, and I've got this listed as "Lepista (Clitocybe) nuda."



Taxonomy is just a way for humans to feel better about themselves. It's all made up!  And more of an art than a science IMO. So use whatever name you like. Hell, I have be using P. pseudo-ulmarius for that "elm" oyster thingie and terry m himself coined that one.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Amanita virosa]
    #19185296 - 11/25/13 01:40 AM (10 years, 2 months ago)

I usually see rings from temp fluctuation if the ambient is too hot/cold for my incubator to control. Not sure they are a clue!


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Re: Identify the Zurich Mystery Culture -- pics! [Re: solarity]
    #19185419 - 11/25/13 03:35 AM (10 years, 2 months ago)

Ok I went through my petris and found the old oyster with the weird obvious rings. Also noticed similar rings in a king oyster market-stall clone and a mild one in one of ten shaggymane plates - but I've got photos of the hyphae aggregates that I don't see in the mystery plates.
got photos of slight brown and reddish discolouring in a plate of Agrocybe aegerita and three Lepista (Clitocybe!) nuda isolates with the typical snow white to bluish grey colouring. I'll have to upload off my phone so give me 24 hours :smile:


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OfflineSkysTheLimit
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19188132 - 11/25/13 05:59 PM (10 years, 2 months ago)

Looks like tree oyster or pink oyster :smile:


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Re: Identify the Zurich Mystery Culture -- pics! [Re: SkysTheLimit]
    #19243624 - 12/08/13 03:58 AM (10 years, 1 month ago)

Here we go:

Weird rings in an unusual Pearl Oyster plate:


No rings and usual fluff and mycelial aggregates in the shaggymane/Corpinus comatus:


Lepista nuda/Clitocybe nuda - a very light lilac tinge but otherwise snowy grey white:


Some more weird rings in a store clone of the king oyster:


And the black poplar just cause i had it in the pic folder:


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OfflineAmanita virosa
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19243833 - 12/08/13 07:19 AM (10 years, 1 month ago)



Not really a wood ear btw!  Terry remembers this nightmare!


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Amanita virosa]
    #19253968 - 12/10/13 05:25 AM (10 years, 1 month ago)

Lentinus polychrous ?...just a feeling :cool: !


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: pilz-kultur]
    #19274606 - 12/14/13 12:39 PM (10 years, 1 month ago)

Well, I finally tracked down the source and species. I messed up with my record keeping. But I'll keep the contest going!

I'll provide a hint: it's a tropical. I know this because I killed it in the regular culture fridge. :foreheadslap:
Fortunately, my source will send me another culture.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19274785 - 12/14/13 01:33 PM (10 years, 1 month ago)

V.volvacea?


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Versicolor]
    #19274841 - 12/14/13 01:51 PM (10 years, 1 month ago)

My V. volva turned somewhat dark grey.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19277699 - 12/15/13 04:15 AM (10 years, 1 month ago)

Tropical? Have to be a paddy or Calocybe indica? Otherwise I'm stumped :smile: I've never seen either on agar so a total guess


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: pilz-kultur]
    #19304159 - 12/20/13 05:53 PM (10 years, 1 month ago)

Quote:

pilz-kultur said:
Lentinus polychrous ?...just a feeling :cool: !




You got it!

:awedancexmas: :awedancexmas: :awedancexmas: :awedancexmas: :awedancexmas:


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Invisiblepilz-kultur
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19306917 - 12/21/13 08:40 AM (10 years, 1 month ago)

Yessssss  :dancer:  :dancer: :dancer:

What did I win Terry ? :wink:

Greets
Walter


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: pilz-kultur]
    #19309639 - 12/21/13 09:39 PM (10 years, 1 month ago)

Quote:

pilz-kultur said:
Yessssss  :dancer:  :dancer: :dancer:

What did I win Terry ? :wink:

Greets
Walter




How about your choice of a couple of edibles from my list?


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OfflineForresterM
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19310693 - 12/22/13 06:10 AM (10 years, 1 month ago)

Geez Terry you could run a professional culture bank with a list like that :lol:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19316009 - 12/23/13 12:40 PM (10 years, 1 month ago)

Quote:

Terry M said:
Quote:

pilz-kultur said:
Yessssss  :dancer:  :dancer: :dancer:

What did I win Terry ? :wink:

Greets
Walter




How about your choice of a couple of edibles from my list?





What a great offer Terry - Thank you !
I :pm: you !


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Re: Identify the Zurich Mystery Culture -- pics! [Re: pilz-kultur]
    #19316301 - 12/23/13 01:56 PM (10 years, 1 month ago)

Quote:

pilz-kultur said:
Lentinus polychrous ?...just a feeling :cool: !




      :lol:


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Sporulator]
    #19316699 - 12/23/13 03:23 PM (10 years, 1 month ago)

Grüezi :cool: !


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Re: Identify the Zurich Mystery Culture -- pics! [Re: pilz-kultur]
    #19319478 - 12/24/13 02:05 AM (10 years, 1 month ago)

Terry - impressive list! Have you tried the orange peel fungi to eat? It grows wild in my yard most years.

would you be interested in adding Marasmius oreades and Lepista saeva to your list?


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OfflineTerry M
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Re: Identify the Zurich Mystery Culture -- pics! [Re: Zen Peddler]
    #19319626 - 12/24/13 03:43 AM (10 years, 1 month ago)

I'm really interested in the Lepista saeva. I got a spore print of this a couple of years ago, but have been unable to grow a clean culture. Interested in Marasmius oreades too, as I always seem to miss collecting these for cloning during the season here.


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Re: Identify the Zurich Mystery Culture -- pics! [Re: Terry M]
    #19324319 - 12/25/13 03:34 AM (10 years, 1 month ago)

I'm in the process of cleaning up and isolating both. When they are up to scratch id trade both for a chicken culture in a heart beat :smile:


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