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OfflineMaeon
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Registered: 11/19/13
Posts: 2
Last seen: 10 years, 2 months
Liquid Culture growth
    #19177799 - 11/23/13 05:57 AM (10 years, 2 months ago)

I have 9 liquid cultures, all prepared with 9 grams Karo (light with red label) and 250ml distilled water. They all have a .2 mu filter patch and are kept between 80 and 82 degrees.

It has been 2 weeks tomorrow and I've not seen any growth.

Suggestions?


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OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Re: Liquid Culture growth [Re: Maeon]
    #19177812 - 11/23/13 06:11 AM (10 years, 2 months ago)

Bin it.


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The ego is nothing other than the focus of conscious attention.


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Offlineblueconfusion
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Registered: 12/14/12
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Re: Liquid Culture growth [Re: Maeon]
    #19177820 - 11/23/13 06:15 AM (10 years, 2 months ago)

I'm sorry to say but LCs are not the way to go try agar or grain transfers LCs are known for being sketchy and there is really only one way to test them and that is a test jar of grain brf or agar.  You should especially not try LCs if you are doing a MS inoculation. I used to do LCs and I had some success with them but lost everything to a contamination after a few inoculations.


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Offlineblueconfusion
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Re: Liquid Culture growth [Re: blueconfusion]
    #19177823 - 11/23/13 06:18 AM (10 years, 2 months ago)



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