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InvisiblebodhisattaMDiscordReddit
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Too slow for agar to grain?
    #19174671 - 11/22/13 12:03 PM (10 years, 3 months ago)


12 days ago


7 days ago


Today.

I had to roll it around a bit to get a good picture, but that ball of myc is all that wedge has done in 12 days.

The other 9 jars are also going this slow. The culture I used has been a proven good performer for me in the past.

Let them keep going? what's the opinion.


Edited by Trusted cuItivator (11/22/13 12:05 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19174736 - 11/22/13 12:31 PM (10 years, 3 months ago)

I'd start more jars buddy, doesn't sound good


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InvisiblebodhisattaMDiscordReddit
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Re: Too slow for agar to grain? [Re: cronicr]
    #19174951 - 11/22/13 01:53 PM (10 years, 3 months ago)

Yea I wonder what's going on.


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175005 - 11/22/13 02:09 PM (10 years, 3 months ago)

I would do agar-LC-grain


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InvisiblebodhisattaMDiscordReddit
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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175012 - 11/22/13 02:10 PM (10 years, 3 months ago)

Quote:

MUSH HEAD420 said:
I would do agar-LC-grain




so I can wait for the LC to colonize and add a vector for contamination? I'd rather do the waiting time in the grains.


Edited by Trusted cuItivator (11/22/13 02:11 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175055 - 11/22/13 02:27 PM (10 years, 3 months ago)

agar-LC-grain is faster and more efficient than agar-grain, just my opinion.


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175069 - 11/22/13 02:32 PM (10 years, 3 months ago)

:strokebeard:


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InvisiblebodhisattaMDiscordReddit
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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175074 - 11/22/13 02:33 PM (10 years, 3 months ago)

Quote:

MUSH HEAD420 said:
agar-LC-grain is faster and more efficient than agar-grain, just my opinion.



fair enough, but I'm never using needles again.

but are these going to slow to keep?


Edited by Trusted cuItivator (11/22/13 02:42 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175088 - 11/22/13 02:38 PM (10 years, 3 months ago)

Quote:

bodhisatta said:
Quote:

MUSH HEAD420 said:
agar-LC-grain is faster and more efficient than agar-grain, just my opinion.



fair enough.

but are these going to slow to keep?



I disagree.

Making an LC is just an unneeded step if you plan on inoculating grain jars in the end anyways.

You could knock up 10 grain jars with agar wedges and G2G them out....without the need for needles and other vectors for contams.

If you already have a culture on agar, you are taking a step backwards IMHO if you make an LC just to inoculate grains jars.

Cut a wedge and just inoculate a grain jar...there is no need to switch to a liquid medium only to go back to grains.....it's quite silly actually.


Edited by PussyFart (11/22/13 02:38 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Too slow for agar to grain? [Re: PussyFart]
    #19175094 - 11/22/13 02:39 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

bodhisatta said:
Quote:

MUSH HEAD420 said:
agar-LC-grain is faster and more efficient than agar-grain, just my opinion.



fair enough.

but are these going to slow to keep?



I disagree.

Making an LC is just an unneeded step if you plan on inoculating grain jars in the end anyways.

You could knock up 10 grain jars with agar wedges and G2G them out....without the need for needles and other vectors for contams.

If you already have a culture on agar, you are taking a step backwards IMHO if you make an LC just to inoculate grains jars.

Cut a wedge and just inoculate a grain jar...there is no need to switch to a liquid medium only to go back to grains.....it's quite silly actually.




I was just about to PM you to have you chime in. But anyway my real question is are these jars of mine going to slow from an agar start to bother keeping?

For the record I'm not doing LC anytime, but I agree that mush head has his opinions of LC and I have mine. I'm never going back to them


Edited by Trusted cuItivator (11/22/13 02:41 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175099 - 11/22/13 02:41 PM (10 years, 3 months ago)

I think they are not contamed, just the one single inoculation point is making it take longer.

To save them, shake until the ball of mycelium is buried under a good amount of grain, you can put it next to the glass on the side of the jar so you can see it's progress. When it is around 10% colonized you can then give it a hard shake and then wait till 100% or shake once more at 60-80%.


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175109 - 11/22/13 02:43 PM (10 years, 3 months ago)

I have a feeling it could be temperature related too. It's been getting as low as 65F some nights in my house.


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175168 - 11/22/13 02:57 PM (10 years, 3 months ago)

Usually when doing agar you want as few genes as possible, meaning as few spores germinating as possible.

Pick out the best sector of the best dish and either put that into one master jar of grain or into a LC for use in nocking up many more jars in a weeks time.

Another rout could be to, pick out the best sector of the best dish and then make an agar dish with the sector, that way your working with a whole colonized agar plate of your isolate. This way you can knock up more master jars or enough for a couple monos.

All I'm trying to point out is that with agar-grain it can take a while before your master jar is done, in that time you could have made a LC and nocked up enough for your whole project.


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175174 - 11/22/13 02:58 PM (10 years, 3 months ago)

If you've G2G'd and/or transferred a lot already, it may just be at the end of it's life.

Due to genetics, some will die out sooner than others. They can only go through so many cell divisions.

I honestly don't think it's your temperature.


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175179 - 11/22/13 03:00 PM (10 years, 3 months ago)

The wedges I used are a few generations of transfers starting from clone tissue. It's not old enough to be senescence(I hope) yet it was a MS fruit to agar to a new agar plate to a new agar plate and that to the grain jar.

I actually used wedges from the plate previous to the plate used on these jars and it did just fine in my last run of grain jars. This is even more limited genetics than the last plate.

I've just never had jars go this slow from agar, ever.


Edited by Trusted cuItivator (11/22/13 03:03 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175185 - 11/22/13 03:02 PM (10 years, 3 months ago)

With a clone you can use the whole agar plate after the first transfer.

You do not need to transfer it a 3rd time.


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175189 - 11/22/13 03:04 PM (10 years, 3 months ago)

Quote:

MUSH HEAD420 said:
With a clone you can use the whole agar plate after the first transfer.

You do not need to transfer it a 3rd time.




I was trying to get an isolate.

Maybe they just need time since I used such small wedges. The wedges were at most the size of a dime.


Edited by Trusted cuItivator (11/22/13 03:05 PM)


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175216 - 11/22/13 03:12 PM (10 years, 3 months ago)

https://www.shroomery.org/forums/showflat.php/Number/17947820#17947820

Quote:

RogerRabbit said:
If a fruit has multiple strains active, removing one might just ruin the whole thing since they're working in concert.
RR




Since you transferred from an MS clone, more than once, then you may have reduced the genetics, thus causing a disruption in the growth.

I know RR is talking about fruiting and your issue is colonization, but they might be similar. I wished I could be more help.

Are your grains pretty dry?


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Re: Too slow for agar to grain? [Re: bodhisatta]
    #19175235 - 11/22/13 03:15 PM (10 years, 3 months ago)

That's another reason why
Quote:

bodhisatta said:
Quote:

MUSH HEAD420 said:
With a clone you can use the whole agar plate after the first transfer.

You do not need to transfer it a 3rd time.




I was trying to get an isolate.






Your isolate was the fruit you cloned, you transfer once if you are worried about contamination from the fruit sample.

I'm guessing you were worried about achieving rhyzo growth. Which in my opinion is pointless.


Edited by MUSH HEAD420 (11/22/13 03:20 PM)


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Re: Too slow for agar to grain? [Re: MUSH HEAD420]
    #19175270 - 11/22/13 03:25 PM (10 years, 3 months ago)

A fruit from a MS grow has multiple genetics.

A clone from a MS grow will not likely be an isolate.

An isolate made from a clone of a MS fruit can result in failure because the multiple genetics are no longer working in concert.


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