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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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What to do with original clone plates
#19173171 - 11/22/13 12:06 AM (10 years, 2 months ago) |
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I tried my hand at cloning a couple weeks ago, and had good luck with no contamination isolating from the mature fruits. I picked the best looking mycelium for a second transfer and those cultures are doing great. I still have my clean original first innoc plates, so are they useful for anything? Do you just toss them once you see your subsequent transfers are doing well?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: What to do with original clone plates [Re: dr.alkaline]
#19173174 - 11/22/13 12:08 AM (10 years, 2 months ago) |
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Throw them to grains and go from there...see what happens
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wildernessjunkie
Reshitivest



Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: What to do with original clone plates [Re: dr.alkaline]
#19173179 - 11/22/13 12:09 AM (10 years, 2 months ago) |
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If they are clean, then you can grow with them. Toss some wedges in some grain jars.
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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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Okay, so at what point does the plate become unusable? can I still slice out sections of mycelium once it is fully colonized? I will not be able to put together a project for a the next month or two, so I threw those bad boys in the fridge to slow them down.
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wildernessjunkie
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Registered: 06/13/10
Posts: 8,118
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Re: What to do with original clone plates [Re: dr.alkaline]
#19173239 - 11/22/13 12:27 AM (10 years, 2 months ago) |
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It becomes unusable if it is contaminated or is used up.
Ill put a sliver from a slant on a plate. Wait for it to grow to the edge of the plate, and then cut the plate into sections depending on how many jars I have to inoculate.
As long as the plates are clean, you can use them to grow, until the plate is empty.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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I've not seen mycelium become weakened by a couple weeks on a fully colonized plate at room temp.
Although there is always the possibility of contams slipping in and landing on the mycelium, just waiting to get knocked on to the grains when you toss the wedge in. For this reason, do not use the agar around the outer 1/4" of the dish
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: What to do with original clone plates [Re: dr.alkaline]
#19177008 - 11/22/13 11:45 PM (10 years, 2 months ago) |
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Quote:
dr.alkaline said: I will not be able to put together a project for a the next month or two, so I threw those bad boys in the fridge to slow them down. 
I should mention that you need to take extra precautions in the fridge.
Wrap the dish very well. Put it in a ziplock bag. Put this inside an insulated lunchbox to prevent condensation due to temp swings as your fridge cycles on and off.
When you come back to it in a month or two, you should take transfers from the colonized plate before inoculating grains. Grow out the mycelium on a new plate and make sure it is clean and viable. Then knock up some jars
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