Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinel2obinn
Stranger
Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
Inoculation hole is too small, have a question about sterilization
    #19170434 - 11/21/13 02:46 PM (10 years, 3 months ago)

This is my first time growing and I realized after sterilizing in a pressure cooker that I made the inoculation holes too small. I underestimated the size of the syringe needle just enough that it wont go in.

What I did to compensate was grab a larger nail and hammer, sprayed both down with a disinfecting spray, and put the nail over a flame for about 20-30 seconds. After I flamed the nail, I wiped it down with alcohol and proceeded to hammer the nail in just enough so that it would widen the inoculation hole, put the foil over the lid and continued to repeat for every jar I had.

Once I did that, I began my inoculations. On a scale of 1 to holy shit you fucked up... how much did I fuck up, and what is the probability of contamination?


Extras: Filter Print Post Top
OfflineTheGreenBox
Male

Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
Re: Inoculation hole is too small, have a question about sterilization [Re: l2obinn]
    #19170504 - 11/21/13 03:03 PM (10 years, 3 months ago)

You didn't fuck up that bad haha... do you have a layer of dry vermiculite on top? If so, the contam probability is a lot less. It sounds like you've been very clean. Should be fine as long as you properly flame sterilized your needle as well and took other precautions!


--------------------
And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless.

- Tool


Extras: Filter Print Post Top
OfflineChakatron
JOOSA JOOSA
Male


Registered: 07/09/13
Posts: 1,646
Loc: Your Face Flag
Last seen: 4 months, 12 days
Re: Inoculation hole is too small, have a question about sterilization [Re: TheGreenBox]
    #19170551 - 11/21/13 03:13 PM (10 years, 3 months ago)

:goodluck:


--------------------


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Inoculation hole is too small, have a question about sterilization [Re: Chakatron]
    #19170732 - 11/21/13 03:39 PM (10 years, 3 months ago)

You're probably okay.

For future reference though...don't wipe your needle after flame sterilizing. Your chance of success would increase if you made the hole bigger while the nail was still hot. Once you flame sterilize something, it is no longer sterile if you wipe alcohol on it.

But, you are probably okay because of your contam barrier.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Offlinel2obinn
Stranger

Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
Re: Inoculation hole is too small, have a question about sterilization [Re: SpitballJedi]
    #19170846 - 11/21/13 03:58 PM (10 years, 3 months ago)

Yeah I had a layer of dry vermiculite on top as well, and for the alcohol wipes I saw that on one of the how to grow videos and it made sense to me to flame, and then use alcohol to cool the needle down as well as wipe off any dead contams on the needle. I was a microbiology student so sterilization comes naturally for me :smile:


Extras: Filter Print Post Top
Offline1down5up
Social Ninja
Male User Gallery

Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
Re: Inoculation hole is too small, have a question about sterilization [Re: l2obinn]
    #19170889 - 11/21/13 04:04 PM (10 years, 3 months ago)

I wiped it too...but was told not to....so on the next round, i just burned the fucker till red hot, and the first drop of solution sizzled and cooled it fast.

If you're not sure, you could always knock up some more...?

I'm a total newb myself, so i can't say for certain that you won't get any contams...but it seems like you took some precaution to prevent it, so good luck! 

after you made larger holes, you coulda  steamed'em again...


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


Extras: Filter Print Post Top
Offlinel2obinn
Stranger

Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
Re: Inoculation hole is too small, have a question about sterilization [Re: 1down5up]
    #19170935 - 11/21/13 04:13 PM (10 years, 3 months ago)

Yeah I could have just made larger holes and re-sterilize them but ain't nobody got time for that. Plus I was really excited because the syringes arrived in the mail the day I found out the holes were too small and I really really really really didn't want to start over.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* PF Tek - Tape On Holes Question fireworks_godS 10,172 5 04/20/02 09:59 AM
by fireworks_god
* Tape Over Inoculation Holes? plaastikbob 4,107 10 09/07/01 10:33 AM
by DeathCapChick
* Non-Hole-Lid sterilization SpaceCruiser 1,267 1 03/01/02 10:38 AM
by crispyburrito
* inoculation holes durban_poison 778 4 09/21/01 10:43 AM
by MAIA
* Covering the inoculation holes on lid? GreyMatter 1,425 2 03/28/02 03:57 PM
by angryshroom
* Question sterilization questions! phrozendata 898 3 09/17/01 08:21 PM
by oscill8
* inoculating substrate question/comment cnidarium 967 3 08/22/02 03:58 AM
by cnidarium
* HELP! MY HOLES ARE TOO SMALL! Zwieback0 1,201 14 08/29/02 02:33 PM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
787 topic views. 30 members, 190 guests and 60 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.007 seconds on 12 queries.