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l2obinn
Stranger
Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
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Inoculation hole is too small, have a question about sterilization
#19170434 - 11/21/13 02:46 PM (10 years, 3 months ago) |
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This is my first time growing and I realized after sterilizing in a pressure cooker that I made the inoculation holes too small. I underestimated the size of the syringe needle just enough that it wont go in.
What I did to compensate was grab a larger nail and hammer, sprayed both down with a disinfecting spray, and put the nail over a flame for about 20-30 seconds. After I flamed the nail, I wiped it down with alcohol and proceeded to hammer the nail in just enough so that it would widen the inoculation hole, put the foil over the lid and continued to repeat for every jar I had.
Once I did that, I began my inoculations. On a scale of 1 to holy shit you fucked up... how much did I fuck up, and what is the probability of contamination?
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TheGreenBox


Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
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Re: Inoculation hole is too small, have a question about sterilization [Re: l2obinn]
#19170504 - 11/21/13 03:03 PM (10 years, 3 months ago) |
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You didn't fuck up that bad haha... do you have a layer of dry vermiculite on top? If so, the contam probability is a lot less. It sounds like you've been very clean. Should be fine as long as you properly flame sterilized your needle as well and took other precautions!
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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Chakatron
JOOSA JOOSA



Registered: 07/09/13
Posts: 1,646
Loc: Your Face
Last seen: 4 months, 12 days
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Re: Inoculation hole is too small, have a question about sterilization [Re: TheGreenBox]
#19170551 - 11/21/13 03:13 PM (10 years, 3 months ago) |
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Inoculation hole is too small, have a question about sterilization [Re: Chakatron]
#19170732 - 11/21/13 03:39 PM (10 years, 3 months ago) |
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You're probably okay.
For future reference though...don't wipe your needle after flame sterilizing. Your chance of success would increase if you made the hole bigger while the nail was still hot. Once you flame sterilize something, it is no longer sterile if you wipe alcohol on it.
But, you are probably okay because of your contam barrier.
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l2obinn
Stranger

Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
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Re: Inoculation hole is too small, have a question about sterilization [Re: SpitballJedi]
#19170846 - 11/21/13 03:58 PM (10 years, 3 months ago) |
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Yeah I had a layer of dry vermiculite on top as well, and for the alcohol wipes I saw that on one of the how to grow videos and it made sense to me to flame, and then use alcohol to cool the needle down as well as wipe off any dead contams on the needle. I was a microbiology student so sterilization comes naturally for me
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1down5up
Social Ninja


Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
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Re: Inoculation hole is too small, have a question about sterilization [Re: l2obinn]
#19170889 - 11/21/13 04:04 PM (10 years, 3 months ago) |
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I wiped it too...but was told not to....so on the next round, i just burned the fucker till red hot, and the first drop of solution sizzled and cooled it fast.
If you're not sure, you could always knock up some more...?
I'm a total newb myself, so i can't say for certain that you won't get any contams...but it seems like you took some precaution to prevent it, so good luck!
after you made larger holes, you coulda steamed'em again...
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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l2obinn
Stranger

Registered: 11/21/13
Posts: 3
Last seen: 10 years, 2 months
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Re: Inoculation hole is too small, have a question about sterilization [Re: 1down5up]
#19170935 - 11/21/13 04:13 PM (10 years, 3 months ago) |
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Yeah I could have just made larger holes and re-sterilize them but ain't nobody got time for that. Plus I was really excited because the syringes arrived in the mail the day I found out the holes were too small and I really really really really didn't want to start over.
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