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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: bodhisatta]
    #19169362 - 11/21/13 10:42 AM (10 years, 3 months ago)

OH man I feel stupid!
I didnt know you were supposed to put it in while hot , I thought It would just create a hole that wouldnt close up!

I always just put it right next to the spot ( like less than and inch) the cooled it and inoculated


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InvisibleSpitballJedi
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19169526 - 11/21/13 11:23 AM (10 years, 3 months ago)

To nobody in particular:

There are many things that people get away with. There are a number of factors that contribute to the success or fail of a grow.

When someone suggests how to do something, that suggestion is for best chance of success. That does not necessarily mean some other way won't work. It just means it's what you should do to increase your chance of success.

Sometimes it's hard to tell who's opinion to listen to. Some opinions are based on lack of experience and seem logical. That's why you should include searches of posts by Trusted Cultivators.

Until your sterilized media becomes 100% colonized, it is very susceptible to contamination. Sterile conditions need to be maintained.

If you wipe your needle with alcohol, it is no longer sterile. That doesn't mean you are guaranteed to fail, but it does increase you chances of fail.

Again, some people get away with this practice, but to say it's best is absolutely wrong.

Because this topic has been talked to death many times, you can easily find similar debates by using the search feature. In your searches, you may notice a pattern arise,..noobs think wiping after flaming is good practice and people with experience trying to correct them.

For best chances of success, flame sterilize your needle and inoculate in a SAB or flowhood. My opinion is not just being pulled right out of my ass. It's an opinion commonly shared by many TC's and experienced well respect members.


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: SpitballJedi]
    #19169562 - 11/21/13 11:31 AM (10 years, 3 months ago)

The TC's rock and there are others you will notice who post often and post things that many people agree with and work well , I myself am not even there , I like to spout ideas out and thoughts but always try and follow it with its my opinion or thoughts or based on my experience.


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OfflineTheBoomking
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19169859 - 11/21/13 12:32 PM (10 years, 3 months ago)

:popcorn:


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Invisibleelasticaltiger
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19169916 - 11/21/13 12:42 PM (10 years, 3 months ago)

Quote:

Kalypto said:
OH man I feel stupid!
I didnt know you were supposed to put it in while hot , I thought It would just create a hole that wouldnt close up!

I always just put it right next to the spot ( like less than and inch) the cooled it and inoculated




Don't feel stupid. I think everyone does stuff like that. I remember the first time I inoculated jars I blew on the needle to cool it down. :facepalm3:

I got lucky, no contamination.  But after I did more reading I worked up the sack to just go straight from the flame to the port.

There are definitely things in this hobby that are counter intuitive to what our gut and experience tells us.  Most of us have only high school lab experience which didn't involve injecting sterile media.  It's up to each of us to trust that the science should dictate the technique, not our guts.

:havesomescience:


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: elasticaltiger]
    #19169931 - 11/21/13 12:44 PM (10 years, 3 months ago)

Quote:

elasticaltiger said:
Quote:

Kalypto said:
OH man I feel stupid!
I didnt know you were supposed to put it in while hot , I thought It would just create a hole that wouldnt close up!

I always just put it right next to the spot ( like less than and inch) the cooled it and inoculated




Don't feel stupid. I think everyone does stuff like that. I remember the first time I inoculated jars I blew on the needle to cool it down. :facepalm3:

I got lucky, no contamination.  But after I did more reading I worked up the sack to just go straight from the flame to the port.

There are definitely things in this hobby that are counter intuitive to what our gut and experience tells us.  Most of us have only high school lab experience which didn't involve injecting sterile media.  It's up to each of us to trust that the science should dictate the technique, not our guts.

:havesomescience:





True that


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InvisibleSpitballJedi
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19170598 - 11/21/13 03:21 PM (10 years, 3 months ago)

Mistakes and lack of knowledge don't make you stupid. The inability to learn is what makes a person stupid. You're not stupid.


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OfflineKizzle
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Re: Inoculating safely without a SAB [Re: SpitballJedi]
    #19171485 - 11/21/13 05:50 PM (10 years, 3 months ago)

Quote:

OH man I feel stupid!
I didnt know you were supposed to put it in while hot , I thought It would just create a hole that wouldnt close up!

I always just put it right next to the spot ( like less than and inch) the cooled it and inoculated



If you're using a self-sealing port excessive heat can damage them. It can still be hot, just needs to cool enough so it's not red-hot. Heat tolerance may vary depending on what it's made of though.


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OfflinePussyFart
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Re: Inoculating safely without a SAB [Re: Kizzle]
    #19171490 - 11/21/13 05:51 PM (10 years, 3 months ago)

Cool the needle BEFORE penetrating the SHIP....exponge a drop or 2 until you hear a sizzle, then inject.

That split second or 2 is not going to matter.


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InvisiblebodhisattaMDiscordReddit
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Re: Inoculating safely without a SAB [Re: PussyFart]
    #19172093 - 11/21/13 07:46 PM (10 years, 3 months ago)

I had always gone red hot right through SHIP when I used needles. SHIP is made of silicone or RTV they both withstand the heat very well. Perhaps it's not the best way, but it works and SHIP's have a lifetime of needle pokes anyway, they're super easy to tear out and replace.


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InvisibleSpitballJedi
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Re: Inoculating safely without a SAB [Re: bodhisatta]
    #19172423 - 11/21/13 08:10 PM (10 years, 3 months ago)

I put the red hot needle through the SHIP and it would only last a few runs before I had a hole you could drip water through.

It's funny to look back on the stuff I used to do to reduce contamination risk. Looking back, I can see how it was actually my technique that I needed to work on. A SHIP was never gonna save me. MS was always a roll of the dice.

Now I see other people going through the same learning process. Some things can't be taught, they have to be learned through experience.

The 2 best decisions I ever made was getting in to agar and proper pasteurization.

Some of you may think I'm an asshole sometimes, but if you ever only take my word on one thing, make it this:

If you want to be successful, do what successful people do, even if you don't understand why. Learn to recognize who the successful people are. Perfect technique and don't rely on chemicals. Respect the process.

Okay, that was 5 things. If you ever only take my word on 5 things, make it that stuff.


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A little civility goes a long way

The Noob Forum
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OfflineKizzle
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Re: Inoculating safely without a SAB [Re: bodhisatta]
    #19172724 - 11/21/13 09:22 PM (10 years, 3 months ago)

Quote:

bodhisatta said:
I had always gone red hot right through SHIP when I used needles. SHIP is made of silicone or RTV they both withstand the heat very well. Perhaps it's not the best way, but it works and SHIP's have a lifetime of needle pokes anyway, they're super easy to tear out and replace.



Me too, but it's because the needle had always cooled enough by the time it reached the jar (maybe 3-4 seconds). After hearing claims it could damage them I was able to intentionally burn one by flaming the needle closer to a lid to verify it can happen.


--------------------


Edited by Kizzle (11/21/13 09:27 PM)


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: PussyFart]
    #19181747 - 11/24/13 08:37 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Cool the needle BEFORE penetrating the SHIP....exponge a drop or 2 until you hear a sizzle, then inject.

That split second or 2 is not going to matter.



Awesome to know I have not been screwing my grows with this


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OfflineKizzle
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19182497 - 11/24/13 12:42 PM (10 years, 3 months ago)

You use an SAB anyway though don't you Kalypto? The time to reach into but should be enough for it to cool but I suppose it depends on exactly how hot you heated it to.


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: Kizzle]
    #19182662 - 11/24/13 01:27 PM (10 years, 3 months ago)

I heat red hot

And yes i still use a SAB unfortunately


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InvisibleusnXe6K

Registered: 12/12/10
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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19182670 - 11/24/13 01:30 PM (10 years, 3 months ago)

I always inoculate in open air and have never had any contams.

Maybe you live in some place dirty. I don't know.


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: usnXe6K]
    #19182679 - 11/24/13 01:33 PM (10 years, 3 months ago)

Quote:

usnXe6K said:
I always inoculate in open air and have never had any contams.

Maybe you live in some place dirty. I don't know.





Ugh Ive inoculated being super careful in a SAB taking like every precaution and still got them and you can do it in open air and be fine >.> *pout*


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OfflinePussyFart
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Re: Inoculating safely without a SAB [Re: usnXe6K]
    #19182681 - 11/24/13 01:33 PM (10 years, 3 months ago)

Quote:

Kalypto said:
And yes i still use a SAB unfortunately



I have no idea why that would be unfortunate....

All I use is my SAB and have no plans on building a flow hood.....ever.

All you need is a big ass comfortable SAB and good sterile technique and you can accomplish ANYTHING in this hobby.

Quote:

usnXe6K said:
I always inoculate in open air and have never had any contams.

Maybe you live in some place dirty. I don't know.



With cakes that have dry verm layers and grain jars that have SHIPs you could get away with open air inoculations.

Is it recommended? No.

Is it good practice? No.

With anything else in this hobby(like agar, G2G transfers, etc) it would be pretty stupid not to use a SAB.....


Edited by PussyFart (11/24/13 01:33 PM)


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OfflineKalypto
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Re: Inoculating safely without a SAB [Re: PussyFart]
    #19182689 - 11/24/13 01:35 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

Kalypto said:
And yes i still use a SAB unfortunately



I have no idea why that would be unfortunate....

All I use is my SAB and have no plans on building a flow hood.....ever.

All you need is a big ass comfortable SAB and good sterile technique and you can accomplish ANYTHING in this hobby.

Quote:

usnXe6K said:
I always inoculate in open air and have never had any contams.

Maybe you live in some place dirty. I don't know.



With cakes that have dry verm layers and grain jars that have SHIPs you could get away with open air inoculations.

Is it recommended? No.

Is it good practice? No.

With anything else in this hobby(like agar, G2G transfers, etc) it would be pretty stupid not to use a SAB.....




I would prefer and LFH and I cant get a SAB thats comfortable to work in , Ive made a couple with to no avail


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InvisibleusnXe6K

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Re: Inoculating safely without a SAB [Re: Kalypto]
    #19182713 - 11/24/13 01:42 PM (10 years, 3 months ago)

Like I said before. It probably depends on the dirtiness of your inoculation location.


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