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ghostshadow422
Stranger


Registered: 10/02/13
Posts: 162
Last seen: 8 years, 1 month
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Re: Inoculating safely without a SAB [Re: PussyFart]
#19167475 - 11/20/13 08:46 PM (10 years, 3 months ago) |
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cool, good to know, I was just worried the heat from the needle would kill the LC or mss, thanks bro
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Inoculating safely without a SAB [Re: PussyFart]
#19167478 - 11/20/13 08:47 PM (10 years, 3 months ago) |
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Quote:
Notahacker420 said:
Quote:
ghostshadow422 said: sorry for the jack but I always wondered this anyways, so it's ok to heat the shit out of the needle then just inject spores and/or LC and it won't affect the solution?
No, the spores will be fine.
This has beenis being discussed to death.
agreed
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Inoculating safely without a SAB [Re: PussyFart]
#19167804 - 11/20/13 10:02 PM (10 years, 3 months ago) |
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Quote:
SpitballJedi said: I think you'll find the baggie to be a bigger pain in the ass than a SAB. The baggie is going to suck air in when you handle it, thus suck contams right in. You would likely have better luck in open air.
If anything, I really think the bag would be pushing air out when handled and there would be no vaccuum to suck it back in. Even if it did suck in a tiny bit of air through the one hole with a needle stuck in that hole, you really think that's worse than open air? I was under the impression inoculating in open air was much riskier than in a small enclosed environment. 
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ghostshadow422 said: alcohol from a bottle soaked up into a cotton ball isn't alcohol pads
Good point.
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Notahacker420 said: At least the soot it sterile, the alcohol is not.
Alcohol does not kill mold spores....heat does.
You could be wiping mold spores all over your clean needle....think about it...it makes sense.
So with all this talk about how alcohol is so unsterile and can spread mold spores all over everything, what do all of you use to wipe your jars/injection ports/tables/glove boxes/etc etc with? Does no one here use fucking alcohol anymore? Lol I thought it was a common practice in this hobby and helped keep contams away, not brought them with it!
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I use mine for storage when it's not in use.....
You need to look at this hobby in a different way, with an open mind bro.
I knew that. I was thinking that it could be used for that purpose but, then I thought, well there's big ass holes in the side for shit to fall out and things to get in (mice, moisture, dirt). Idrk what I'd store in a tote like that honestly. Guess ya could tape the holes up or something but, that wasn't my point. I have plenty of totes for storage, I don't need another one taking up room.
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Just dont wipe your needles after flaming...that is just bad practice.
The first few drops of spores will cool the needle, then the rest will flow contam free.
Ok. If you all say that the needle will remain sterile just chillin in open air while it cools and there's no reason to cover it with anything then I won't cover it/wipe it with alcohol. Should I not wipe the top of my jars with alcohol or the outside of the injection site on the bag with alcohol either?? Now I am curious how many of you use alcohol at any point during inoculation/how many don't/and contam rates of jars using alcohol vs no alcohol at all. Maybe that should be a poll.. Anyway, thanks every one. Peace
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Inoculating safely without a SAB [Re: shroomdust]
#19167851 - 11/20/13 10:13 PM (10 years, 3 months ago) |
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It's got me thinking now.. if alcohol is just a "sanitizer" and could possibly carry mold spores, why would it be ok/recommended to wash your hands in it and wipe down jars and everything else in alcohol except for the needle? Doesn't make much sense. I have wiped my jars and needle every single time on probably 60+ jars and only had a handful of contams probably due to something else. Sometimes, not even flaming the needle and simply just wiping with iso. I'm sure I'm not the only one who's used alcohol religiously in this hobby and credited it to his/her success, right?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Inoculating safely without a SAB [Re: shroomdust]
#19167876 - 11/20/13 10:19 PM (10 years, 3 months ago) |
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Quote:
shroomdust said: It's got me thinking now.. if alcohol is just a "sanitizer" and could possibly carry mold spores, why would it be ok/recommended to wash your hands in it and wipe down jars and everything else in alcohol except for the needle? Doesn't make much sense.
Well nobody"s gonna sterilize there hands for one< and secondly when you use alcohol on your hands your killing contact contms not airburne contams< all the more reason to use a sab and not a baggie
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,409
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Re: Inoculating safely without a SAB [Re: shroomdust]
#19167940 - 11/20/13 10:38 PM (10 years, 3 months ago) |
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Quote:
shroomdust said: Alcohol may not kill airborne contams (though I doubt this is entirely true) but, it can PROTECT against them landing on your needle or whatever. Do contams live in alcohol or is alcohol considered sterile/resistant to contamination? The point is that the alcohol soaked paper towel offers a clean covering for the needle before you stick it in the jar and at the same time cools it down so you don't kill all your spores. It couldn't hurt..could only help. Not like it's a chore to do or expensive. It may not be needed but I don't think it's going backwards.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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*Sanitize means to reduce the number of contaminants to a safe or relatively safe level as may be judged by public health requirements. Disinfect means elimination of all recognized pathogenic microorganism but not necessarily all microbial forms. Sterilize means the destruction of all microbial life by use of chemical or physical procedures.
we can wipe the outside of things with alcohol because it doesn't need to be sterile, the minute you take your jar out of your sab(or even before) it's gonna be "contammed" on the outside, anything going in the jar(your needle) should be sterilized
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Inoculating safely without a SAB [Re: cronicr]
#19168038 - 11/20/13 11:10 PM (10 years, 3 months ago) |
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shroomdust
Psilovibin



Registered: 09/04/13
Posts: 114
Last seen: 9 years, 5 months
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Re: Inoculating safely without a SAB [Re: cronicr]
#19168104 - 11/20/13 11:38 PM (10 years, 3 months ago) |
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Quote:
cronicr said:Well nobody"s gonna sterilize there hands for one< and secondly when you use alcohol on your hands your killing contact contms not airburne contams< all the more reason to use a sab and not a baggie
You mean I shouldn't be flame sterilizing my hands in between each jar too?!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Inoculating safely without a SAB [Re: shroomdust]
#19168122 - 11/20/13 11:45 PM (10 years, 3 months ago) |
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no, then you won't have any hands to facepalm yourself lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: Inoculating safely without a SAB [Re: shroomdust]
#19168555 - 11/21/13 03:23 AM (10 years, 3 months ago) |
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Quote:
shroomdust said:
Ok. If you all say that the needle will remain sterile just chillin in open air while it cools and there's no reason to cover it with anything then I won't cover it/wipe it with alcohol.
The thing is you are not just leaving it lying around in open air to cool down for ages...heat to red hot and then quickly and gracefully innoc straight away - the first bit of solution will cool the needle sufficiently whilst it is in a sterile environment (your jar) 
Edit: Quote:
cronicr said: no, then you won't have any hands to facepalm yourself lol
^^ just to say - this actually made me do a little lol
Edited by Skinty (11/21/13 03:25 AM)
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,409
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Re: Inoculating safely without a SAB [Re: Skinty]
#19168578 - 11/21/13 03:41 AM (10 years, 3 months ago) |
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Quote:
Quote: shroomdust said:
Ok. If you all say that the needle will remain sterile just chillin in open air while it cools and there's no reason to cover it with anything then I won't cover it/wipe it with alcohol.
It should take barely a second for you to take the needle from the flame and stick it through the inoculation port. The one second it takes you to take the needle from the flame to port the needle will still be hot enough to kill any mold spores or air born contamination it may come into contact with.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: Inoculating safely without a SAB [Re: PussyFart]
#19168592 - 11/21/13 03:54 AM (10 years, 3 months ago) |
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Quote:
Notahacker420 said: Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Quote:
ghostshadow422 said: you were once a noob too, still an asshole though it seems lol

We were all noobs; being called a noob isn't an insult. I can't help what makes me laugh though. It's funny because I remember being the same way.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Kalypto
Psychonaut



Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
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Re: Inoculating safely without a SAB [Re: PirateSwazey]
#19169018 - 11/21/13 08:43 AM (10 years, 3 months ago) |
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Quote:
PirateSwazey said: i've used a piece of a sponge soaked in alcohol right over my innoc port and had success in open air...even so i would always have to recommend using a SAB or glovebox
Dont use a GB use a SAB especially for how cheap you can get a SAB you will have much more success in it
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sytar
Radiant



Registered: 09/01/13
Posts: 381
Last seen: 9 years, 6 months
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Re: Inoculating safely without a SAB [Re: Kalypto]
#19169103 - 11/21/13 09:16 AM (10 years, 3 months ago) |
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My microbiology classes made alcohol sound far, far more effective at killing microbes than you are all making it out to be. My microbiology professor said that microbes couldn't evolve to become alcohol resistant like they could with antibiotics because dousing them in alcohol is like dropping a nuclear bomb on them.
Not disagreeing that you should just push some solution out of the needle to cool it, but I definitely think the properties of alcohol are being understated.
-------------------- I post from my phone. Excuse the typos and autocorrects.
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Kalypto
Psychonaut



Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
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Re: Inoculating safely without a SAB [Re: sytar]
#19169117 - 11/21/13 09:22 AM (10 years, 3 months ago) |
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Quote:
sytar said: My microbiology classes made alcohol sound far, far more effective at killing microbes than you are all making it out to be. My microbiology professor said that microbes couldn't evolve to become alcohol resistant like they could with antibiotics because dousing them in alcohol is like dropping a nuclear bomb on them.
Not disagreeing that you should just push some solution out of the needle to cool it, but I definitely think the properties of alcohol are being understated.
Alcohol is a surface cleaner so whatever you clean with alcohol may be clean , but as soon as it evaporates off of it , the surface is no longer sterile since contams can land on it , not to mention alcohol does not kill everything .
Flames kill EVERYTHING and remain sterile until you cool it down which is often right before the inoculation
Now I may be wrong but to my knowledge this is why we use flame when possible and alcohol when flame is not an option
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 23 hours
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Re: Inoculating safely without a SAB [Re: sytar]
#19169164 - 11/21/13 09:41 AM (10 years, 3 months ago) |
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Quote:
sytar said: My microbiology classes made alcohol sound far, far more effective at killing microbes than you are all making it out to be. My microbiology professor said that microbes couldn't evolve to become alcohol resistant like they could with antibiotics because dousing them in alcohol is like dropping a nuclear bomb on them.
Not disagreeing that you should just push some solution out of the needle to cool it, but I definitely think the properties of alcohol are being understated.
For alcohol to have it's full effect the contaminant would have to be in direct contact with it for 10 minutes. There's plenty of time for things to get spread around. But the point of the link I posted is to show that there are indeed things whixh can survive alcohol completely.
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Kalypto
Psychonaut



Registered: 09/19/12
Posts: 2,089
Last seen: 2 years, 22 days
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Re: Inoculating safely without a SAB [Re: Kizzle]
#19169171 - 11/21/13 09:44 AM (10 years, 3 months ago) |
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The more you know !
Had no clue about the ten minute thing
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Flame needle = sterile
While it's hot put it into inoculation port
First drops of spores come out as steam the spores die from the heat and cool the needle
The next drop the spores live because the needle is cooled down since a needle is so small it has a very small thermal mass and will not hold heat very long
Or
Flame needle = sterile
Wipe with alcohol = sanitary but has the .01% crap on it now. Chances are you'll be fine but why bother.
It's not rocket science.
It's in the let's grow mushroom videos that you should have watched.
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