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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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First Attempt (*picture update*)
#19165026 - 11/20/13 12:22 PM (10 years, 3 months ago) |
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Greetings! As you can see this is my very first post here in the shroomery, so expect newbish and a lot to fall over.
Well... about a year ago I got into the idea of mushroom cultivation and read about the teks and procedures since then, so I suppose I did my homework quite good. For understandable reasons I decided to go with the PF-Tek but I have to start from print (FSRE).
My equipment: -sterile packaged syringes (10ml) &needles -sterile water for intravenous injection (to make the syringes) -my DIY SAB <---will come to that later -verm and BRF -little pressure cooker -some jars and glasses -diverse alcohols for sanitizing -surgical knives (sterile packaged) I have the problem that my SAB has very large armholes (11cm) and I don't close them with my arms. I used a piece of a PVC pipe and the correct fittings to close while air is settling, but I feat contaminants coming through the holes and fucking up my syringes.
For making the syringe I will pc 3 shot glasses for 120 minutes, wrapped in tin foil of course. Let cool overnight. Next day, after showering, putting on fresh laundered clothes etc., I'll put them in the box together with 3 syringes+needles, 3 vials of the water, a sterile packaged surgical knife and a bottle of iso-alc. Then I'll spray the box and sealed armholes with 70% alc and close the box. After 20 mins or so I'll start the syringe making, sanitizing my arms+hands and putting dishwasher gloves on, which I'll also sanitize. After all 3 syringes are done I'll flame the needle of every syringe outside the box and put the thingy back on it.
I really fear my complete grow getting fucked up because my SAB is shit with that big holes... I need someone to tell me that it'll work out or if I miss something.
*Update* First of all thanks to all you experienced growers helping me out and the shroomery in general.  ALL my jars which are 8 total have a slightly woodlike/sweet smell which comes from the substrate, but since my freshly pced jars smell the same, just less intense, I dont worry about it. Today, after coming home from work I checked the jars I worry most about, the 4cc spore solution each ones. And see there:
 Contamination? I think it looks like myc and it's only under innoc points and at the bottom a bit, which comes from the 4cc innoc collecting at the bottom I suppose x.x. New picture 24 hrs later: (1/4 pint jar)
Edited by mongolian (11/29/13 11:53 AM)
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BillNyeScienceGuy
Activist


Registered: 11/19/13
Posts: 70
Last seen: 1 year, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19165847 - 11/20/13 02:56 PM (10 years, 3 months ago) |
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I am new as well, have been lurking the forums for months and done a lot of homework. I prepared and started just like you, your SAB sounds fine, you can use syran wrap to wrap around your box and then stick your arms in or use some tape to block those holes. I knocked up 3 jars and all 3 are almost about 75% as of today, just do what you said, take shower, clean clothes sterilize box etc. You will be fine as long as you take precaution and care about your work!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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you got this
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: cronicr]
#19168804 - 11/21/13 07:14 AM (10 years, 3 months ago) |
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Thanks for reminding me of the saran wrap, I just got it translated! Always thought that it would be something expensive for advanced growers:D. So that's great thanks.
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
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Re: First Attempt (questions) [Re: mongolian]
#19169195 - 11/21/13 09:52 AM (10 years, 3 months ago) |
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With the PF Tek, if anything is still in your SAB it will get caught by the dry verm layer in your jars. I would'nt sweat that.
Not knowing if your print is clean, well that's another. Make sure you only use the 1/4cc per hole when inoculating. The less fluid you use, the fewer chances of a contam being introduced.
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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TheGreenBox


Registered: 08/03/13
Posts: 276
Last seen: 4 years, 3 months
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I just used saran wrap too for my first SAB. You should be fine!
Just don't forget about flame sterilizing your needle, etc. It is very important.
Good luck!
-------------------- And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless. - Tool
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mongolian
Stranger

Registered: 10/04/13
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Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: TheGreenBox]
#19169258 - 11/21/13 10:18 AM (10 years, 3 months ago) |
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Would it be a problem if I just put the cap back on after syringe making and flame outside when all 3 are done? So that I don't have so much air moving around.
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OneiricOutsider
Drucid Leamer


Registered: 10/05/13
Posts: 136
Loc: In the autumn shade
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Re: First Attempt (questions) [Re: mongolian]
#19169423 - 11/21/13 10:55 AM (10 years, 3 months ago) |
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No that's fine, you can flame the needle whenever after the syringe itself has already been made. Just make sure to give it a good flame before sucking up the spores. And remember that when making the syringes, it's better to use a small amount of spores per syringe, less chance of contamination. I made syringes for the first time last week, and so far so good
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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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I don't think that I have to flame it before sucking up when every single tool I use comes sterile packaged from the pharmacy. Even the water:D. Correct me if I'm wrong...
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OneiricOutsider
Drucid Leamer


Registered: 10/05/13
Posts: 136
Loc: In the autumn shade
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Re: First Attempt (questions) [Re: mongolian]
#19169484 - 11/21/13 11:10 AM (10 years, 3 months ago) |
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Hmm I guess if it's pre-sterilized, then you're good to go haha. I was writing that from the perspective of re-using old inoculation needles.
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mongolian
Stranger

Registered: 10/04/13
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Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19169544 - 11/21/13 11:28 AM (10 years, 3 months ago) |
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Experiments starting tomorrow.
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mongolian
Stranger

Registered: 10/04/13
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Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19181309 - 11/24/13 02:48 AM (10 years, 3 months ago) |
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Got my syringes done without any trouble, will shoot some test jars in 10 hrs when they cooled down! (Made them totally clear, in 1 you can see some tiny clumps but that's all, hoping to avoid any contams)
Edited by mongolian (11/24/13 02:50 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19181313 - 11/24/13 02:53 AM (10 years, 3 months ago) |
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Rootin for ya!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 4 months
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Re: First Attempt (questions) [Re: cronicr]
#19181393 - 11/24/13 03:56 AM (10 years, 3 months ago) |
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-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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Just innoced 1 1/2 and 1/4 pint with about 4ccs each.... Any hope for the cakes? Also I have the problem that some solution drops are coming out of my syringe while trying to shoot the cakes.
Pc'ing another 3 glasses for tomorrow!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19182224 - 11/24/13 11:25 AM (10 years, 3 months ago) |
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Make sure your needle is tight
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: cronicr]
#19182230 - 11/24/13 11:26 AM (10 years, 3 months ago) |
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It is, I mean out of the needle, dripping on my jar and dry verm layer while sticking in D:.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19182251 - 11/24/13 11:30 AM (10 years, 3 months ago) |
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Is there lots of air in it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mongolian
Stranger

Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: cronicr]
#19182259 - 11/24/13 11:32 AM (10 years, 3 months ago) |
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thanks, that could be it! About 1.5 ccs of air.
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mongolian
Stranger

Registered: 10/04/13
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Last seen: 10 years, 1 month
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Re: First Attempt (questions) [Re: mongolian]
#19184816 - 11/24/13 10:39 PM (10 years, 2 months ago) |
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Innoc was way better, but I still have to practice dat syringe handling . The glasses got shot between 1.5 and 2cc's. One accidently got 2.5-3cc's.
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