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Offlinemotts
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Registered: 02/26/03
Posts: 31
Last seen: 10 years, 1 month
Re-Using Jars
    #19163972 - 11/20/13 08:34 AM (10 years, 3 months ago)

Question: I injected some jars with spores. After 3 weeks there was no sign of growth. I followed the PF Tek method 100%. The spores were inactive. I have received new spores. Would it be ok to re inject the jars that are essentially 100% sterilized with the spores that are inactive already in there? Or should I put the jars back into the steamer for 90 minutes and inject them, or just throw away and start fresh?

I didn't think using jars with dead spores was an issue, but wanted some clarity. Thanks.


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Offlinedodgem
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Re: Re-Using Jars [Re: motts]
    #19163994 - 11/20/13 08:44 AM (10 years, 3 months ago)

Throw them away and start fresh.  The spores likely not inactive, but more likely either your sterilization process was off or the spores syringes were contaminated.  There may be some sort of contamination that is tough to see with the naked eye so you would just be doomed for failure again.


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OfflineMAIA
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Re: Re-Using Jars [Re: motts]
    #19167881 - 11/20/13 10:20 PM (10 years, 3 months ago)

I'm with dodgem, just restart the whole thing. You can re-use the jars in a new grow. Dump the substrate, double clean the jars, prepare new substrate, sterilize and repeat inoculation.


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